Abstract
The roles of phosphoinositide 3-kinase (PI3K) and phospholipase C (PLC) in chemoattractant-elicited responses were studied in mice lacking these key enzymes. PI3Kgamma was required for chemoattractant-induced production of phosphatidylinositol 3,4,5-trisphosphate [PtdIns (3,4,5)P3] and has an important role in chemoattractant-induced superoxide production and chemotaxis in mouse neutrophils and in production of T cell-independent antigen-specific antibodies composed of the immunoglobulin lambda light chain (TI-IglambdaL). The study of the mice lacking PLC-beta2 and -beta3 revealed that the PLC pathways have an important role in chemoattractant-mediated production of superoxide and regulation of protein kinases, but not chemotaxis. The PLC pathways also appear to inhibit the chemotactic activity induced by certain chemoattractants and to suppress TI-IglambdaL production.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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B-Lymphocytes / immunology
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Chemokine CCL4
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Chemotactic Factors / pharmacology*
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Chemotaxis, Leukocyte / physiology*
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Immunoglobulin lambda-Chains / biosynthesis
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Isoenzymes / metabolism*
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Macrophage Inflammatory Proteins / pharmacology
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Mice
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N-Formylmethionine Leucyl-Phenylalanine / pharmacology
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Neutrophil Infiltration
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Neutrophils / metabolism
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Neutrophils / physiology*
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Peritonitis / immunology
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Phosphatidylinositol 3-Kinases / metabolism*
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Phosphatidylinositol Phosphates / metabolism
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Phospholipase C beta
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Phosphorylation
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Signal Transduction*
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Skin Ulcer / pathology
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Superoxides / metabolism
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Type C Phospholipases / metabolism*
Substances
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Chemokine CCL4
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Chemotactic Factors
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Immunoglobulin lambda-Chains
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Isoenzymes
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Macrophage Inflammatory Proteins
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Phosphatidylinositol Phosphates
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phosphatidylinositol 3,4,5-triphosphate
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Superoxides
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N-Formylmethionine Leucyl-Phenylalanine
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Phosphatidylinositol 3-Kinases
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Type C Phospholipases
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Phospholipase C beta
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Plcb2 protein, mouse
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Plcb3 protein, mouse