Abstract
Drosophila mei-9 is essential for several DNA repair and recombination pathways, including nucleotide excision repair (NER), interstrand crosslink repair, and meiotic recombination. To better understand the role of MEI-9 in these processes, we characterized 10 unique mutant alleles of mei-9. These include a P-element insertion that disrupts repair functions but not the meiotic function; three nonsense mutations, one of which has nearly wild-type levels of protein; three missense mutations, one of which disrupts the meiotic function but not repair functions; two small in-frame deletions; and one frameshift.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Alleles
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Amino Acid Motifs
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Amino Acid Sequence
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Animals
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Blotting, Western
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Codon, Nonsense
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DNA Damage
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DNA Mutational Analysis*
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DNA Repair*
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Drosophila Proteins / genetics*
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Drosophila Proteins / physiology*
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Drosophila melanogaster / genetics*
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Frameshift Mutation
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Gene Deletion
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Meiosis
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Models, Genetic
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Molecular Sequence Data
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Mutation
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Mutation, Missense
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Nuclear Proteins / genetics*
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Nuclear Proteins / physiology*
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Recombination, Genetic*
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Sequence Homology, Amino Acid
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Two-Hybrid System Techniques
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Ultraviolet Rays
Substances
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Codon, Nonsense
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Drosophila Proteins
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MEI-9 protein, Drosophila
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Nuclear Proteins