Abstract
This chapter introduces a new method of maintaining human embryonic stem cells (hESCs) in the undifferentiated state through treatment with a GSK-3 inhibitor, BIO, under a feeder-free condition. Additionally, methods are introduced that determine multidifferentiation potential of hESCs by differentiating into a specific type or random heterogenous cell populations in vitro. These approaches will become a fundamental platform to identify molecular networks regulating cell fate determination in hESCs and mouse embryonic stem cells.
MeSH terms
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Animals
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Cell Differentiation / drug effects
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Cell Division / drug effects
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Coculture Techniques / methods*
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Enzyme Inhibitors / pharmacology*
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Genes, Reporter
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Glycogen Synthase Kinase 3 / antagonists & inhibitors*
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Humans
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Immunohistochemistry
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Indoles / pharmacology*
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Luciferases / genetics
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Mice
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Neurons / cytology
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Oximes / pharmacology*
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Pluripotent Stem Cells / cytology*
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Pluripotent Stem Cells / drug effects
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Pluripotent Stem Cells / enzymology
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Signal Transduction / drug effects
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Stromal Cells / cytology
Substances
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6-bromoindirubin-3'-oxime
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Enzyme Inhibitors
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Indoles
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Oximes
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Luciferases
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Glycogen Synthase Kinase 3