The G protein coupled to the thromboxane A2 receptor in human platelets is a member of the novel Gq family

J Biol Chem. 1991 May 15;266(14):9309-13.

Abstract

The thromboxane A2 (TXA2) receptor in human platelets is coupled to a pertussis toxin-insensitive G protein whose identity has remained unknown. Candidates for this role include the atypical G protein known as Gz and members of a recently discovered G protein family known as Gq. Because of the proven utility of antibodies directed against the C terminus of G protein alpha subunits as functional probes, we prepared an antibody against a synthetic decapeptide corresponding to the C-terminal sequence shared by alpha 11 and alpha q, two members of the new family. This antibody (QL) does not recognize known alpha subunits but selectively binds to a 42-kDa protein in a variety of tissues, including human platelet membranes. QL and two other C-terminal antibodies, QN and AS, known to recognize alpha z and alpha i2, respectively, were tested for their ability to block agonist-stimulated GTPase activity in human platelet membranes. Pretreatment of platelet membranes with AS has previously been shown to interfere with alpha 2 adrenergic receptor-mediated inhibition of adenylylcyclase. As expected, only AS antibody produced inhibition of alpha 2 receptor-stimulated GTPase. Pretreatment of membranes with QL, but not QN or AS, caused marked inhibition of TXA2 receptor-stimulated GTPase. This identifies the G protein coupled to human platelet TXA2 receptors as a member of the novel Gq family.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Blood Platelets / metabolism*
  • Blotting, Western
  • Brain / metabolism
  • Cattle
  • Cell Membrane / metabolism
  • GTP Phosphohydrolases / metabolism
  • GTP-Binding Proteins / classification
  • GTP-Binding Proteins / immunology
  • GTP-Binding Proteins / metabolism*
  • Humans
  • In Vitro Techniques
  • Molecular Sequence Data
  • Prostaglandin Endoperoxides, Synthetic / pharmacology
  • Receptors, Adrenergic, alpha / physiology
  • Receptors, Prostaglandin / metabolism*
  • Receptors, Thromboxane
  • Signal Transduction
  • Thromboxane A2 / metabolism

Substances

  • Prostaglandin Endoperoxides, Synthetic
  • Receptors, Adrenergic, alpha
  • Receptors, Prostaglandin
  • Receptors, Thromboxane
  • Thromboxane A2
  • GTP Phosphohydrolases
  • GTP-Binding Proteins