Abstract
A genomic clone encoding human fast-twitch skeletal muscle calsequestrin was isolated, and the amino acid sequence of the protein and the exon-intron boundaries of the gene were deduced from its sequence. A comparison with the rabbit gene showed that the sequence Glu-Asp-Asp-Asp-Asp near the COOH terminus of the rabbit sequence is lacking in the human gene. The calsequestrin gene was assigned to human chromosome 1 through the use of a human-mouse somatic cell hybrid mapping panel.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Calsequestrin / analysis
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Calsequestrin / genetics*
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Chromosome Mapping
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Chromosomes, Human, Pair 1 / ultrastructure*
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DNA / analysis
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DNA / genetics
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Genes / genetics*
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Humans
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Hybrid Cells / analysis
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Hybrid Cells / cytology
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Hybrid Cells / ultrastructure
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Molecular Sequence Data
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Muscle Proteins / genetics*
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Muscles / analysis*
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Muscles / physiology
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Rodentia
Substances
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Calsequestrin
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Muscle Proteins
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DNA