Abstract
Transcriptional activation of the human interleukin-2 (IL-2) gene, like induction of the IL-2 receptor alpha (IL-2R alpha) gene and the type 1 human immunodeficiency virus (HIV-1), is shown to be modulated by a kappa B-like enhancer element. Mutation of a kappa B core sequence identified in the IL-2 promoter (-206 to -195) partially inhibits both mitogen- and HTLV-I Tax-mediated activation of this transcription unit and blocks the specific binding of two inducible cellular factors. These kappa B-specific proteins (80 to 90 and 50 to 55 kilodaltons) similarly interact with the functional kappa B enhancer present in the IL-2R alpha promoter. These data suggest that these kappa B-specific proteins have a role in the coordinate regulation of this growth factor-growth factor receptor gene system that controls T cell proliferation.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Base Sequence
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Cell Line
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Cloning, Molecular
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DNA / metabolism
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DNA-Binding Proteins / metabolism*
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Enhancer Elements, Genetic*
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Gene Expression Regulation*
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Genes, Viral
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HIV-1 / genetics
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HTLV-I Antigens / pharmacology
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Humans
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Immunoglobulin kappa-Chains / genetics*
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Interleukin-2 / genetics*
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Molecular Weight
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Mutation
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Phytohemagglutinins / pharmacology
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Plasmids
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Promoter Regions, Genetic
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RNA, Messenger / biosynthesis
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T-Lymphocytes / metabolism
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Tetradecanoylphorbol Acetate / pharmacology
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Trans-Activators
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Transcription Factors / pharmacology
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Transcription, Genetic
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Transfection
Substances
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DNA-Binding Proteins
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HTLV-I Antigens
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Immunoglobulin kappa-Chains
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Interleukin-2
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Phytohemagglutinins
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RNA, Messenger
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Trans-Activators
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Transcription Factors
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DNA
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Tetradecanoylphorbol Acetate