Abstract
A transhydrogenase that catalyzes the oxoacid-dependent oxidation of specific hydroxyacids has been found in rat kidney, liver, and brain. The hydroxyacids that have been found to be substrates for this enzyme are gamma-hydroxybutyrate, D-alpha-hydroxyglutarate, and L-beta-hydroxybutyrate. The oxoacids that are the best substrates for this enzyme are alpha-ketoglutarate and succinic semialdehyde; alpha-ketoadipate and oxalacetate are also substrates. This enzyme is located in the mitochondrial fraction of the cell and is not dependent on added NAD+ or NADP+.
MeSH terms
-
3-Hydroxybutyric Acid
-
Animals
-
Chromatography, Paper
-
Gas Chromatography-Mass Spectrometry
-
Glutarates / metabolism
-
Hydrogen-Ion Concentration
-
Hydroxybutyrates / metabolism
-
Kidney / enzymology*
-
Kidney / ultrastructure
-
Kinetics
-
Mitochondria / enzymology
-
NAD / metabolism
-
NADP / metabolism
-
Rats
-
Sodium Oxybate / metabolism
-
Time Factors
-
gamma-Aminobutyric Acid / analogs & derivatives
-
gamma-Aminobutyric Acid / metabolism
Substances
-
Glutarates
-
Hydroxybutyrates
-
NAD
-
alpha-hydroxyglutarate
-
NADP
-
gamma-Aminobutyric Acid
-
Sodium Oxybate
-
succinic semialdehyde
-
3-Hydroxybutyric Acid