Cloning and characterization of the Drosophila melanogaster CDK5 homolog

FEBS Lett. 1994 Dec 19;356(2-3):317-21. doi: 10.1016/0014-5793(94)01298-9.

Abstract

The D. melanogaster homolog of mammalian CDK5 has been cloned and its chromosomal location determined. The gene for Cdk5 consists of 4 exons separated by 3 short introns ranging in size from 61-160 bp. Northern blot analysis revealed a single mRNA of approximately 1.6 kb that is expressed at highest levels in the adult fly. The putative amino acid sequence for Drosophila Cdk5 predicts a protein with a mass of approximately 32 kDa that is 77% identical to its mammalian counter-parts. Drosophila Cdk5 gene is located in polytene chromosomal region 52BC of the right arm of chromosome 2. This study provides the framework for a molecular genetic analysis of CDK5 function.

Publication types

  • Comparative Study

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Blotting, Northern
  • Blotting, Southern
  • Cattle
  • Chromosome Mapping
  • Cloning, Molecular
  • Cyclin-Dependent Kinase 5
  • Cyclin-Dependent Kinases*
  • DNA Primers
  • Drosophila Proteins
  • Drosophila melanogaster / metabolism*
  • Gene Amplification
  • Gene Library
  • Humans
  • In Situ Hybridization
  • Introns
  • Mammals
  • Molecular Sequence Data
  • Polymerase Chain Reaction
  • Protein Serine-Threonine Kinases / biosynthesis*
  • Protein Serine-Threonine Kinases / genetics*
  • RNA, Messenger / analysis
  • RNA, Messenger / biosynthesis
  • Rats
  • Restriction Mapping
  • Salivary Glands / enzymology
  • Sequence Homology, Amino Acid

Substances

  • DNA Primers
  • Drosophila Proteins
  • RNA, Messenger
  • Cyclin-Dependent Kinase 5
  • Protein Serine-Threonine Kinases
  • CDK5 protein, human
  • Cdk5 protein, Drosophila
  • Cdk5 protein, rat
  • Cyclin-Dependent Kinases

Associated data

  • GENBANK/U21552