Abstract
The D. melanogaster homolog of mammalian CDK5 has been cloned and its chromosomal location determined. The gene for Cdk5 consists of 4 exons separated by 3 short introns ranging in size from 61-160 bp. Northern blot analysis revealed a single mRNA of approximately 1.6 kb that is expressed at highest levels in the adult fly. The putative amino acid sequence for Drosophila Cdk5 predicts a protein with a mass of approximately 32 kDa that is 77% identical to its mammalian counter-parts. Drosophila Cdk5 gene is located in polytene chromosomal region 52BC of the right arm of chromosome 2. This study provides the framework for a molecular genetic analysis of CDK5 function.
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Blotting, Northern
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Blotting, Southern
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Cattle
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Chromosome Mapping
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Cloning, Molecular
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Cyclin-Dependent Kinase 5
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Cyclin-Dependent Kinases*
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DNA Primers
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Drosophila Proteins
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Drosophila melanogaster / metabolism*
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Gene Amplification
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Gene Library
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Humans
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In Situ Hybridization
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Introns
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Mammals
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Molecular Sequence Data
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Polymerase Chain Reaction
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Protein Serine-Threonine Kinases / biosynthesis*
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Protein Serine-Threonine Kinases / genetics*
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RNA, Messenger / analysis
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RNA, Messenger / biosynthesis
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Rats
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Restriction Mapping
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Salivary Glands / enzymology
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Sequence Homology, Amino Acid
Substances
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DNA Primers
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Drosophila Proteins
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RNA, Messenger
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Cyclin-Dependent Kinase 5
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Protein Serine-Threonine Kinases
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CDK5 protein, human
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Cdk5 protein, Drosophila
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Cdk5 protein, rat
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Cyclin-Dependent Kinases