Abstract
The Pto gene in tomato confers resistance to races of Pseudomonas syringae pv. tomato that carry the avirulence gene avrPto. A yeast artificial chromosome clone that spans the Pto region was identified and used to probe a leaf complementary DNA (cDNA) library. A cDNA clone was isolated that represents a gene family, at least six members of which genetically cosegregate with Pto. When susceptible tomato plants were transformed with a cDNA from this family, they were resistant to the pathogen. Analysis of the amino acid sequence revealed similarity to serine-threonine protein kinases, suggesting a role for Pto in a signal transduction pathway.
Publication types
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Amino Acid Sequence
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Chromosomes, Artificial, Yeast
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Cloning, Molecular*
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DNA, Complementary / genetics
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Genes, Plant*
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Molecular Sequence Data
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Multigene Family*
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Plant Diseases / genetics*
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Plant Proteins*
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Polymorphism, Restriction Fragment Length
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Protein Serine-Threonine Kinases / chemistry
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Protein Serine-Threonine Kinases / genetics*
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Protein Serine-Threonine Kinases / metabolism
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Pseudomonas / pathogenicity
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Signal Transduction
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Vegetables / enzymology
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Vegetables / genetics*
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Vegetables / microbiology
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Virulence
Substances
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DNA, Complementary
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Plant Proteins
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Pto protein, Lycopersicon
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Protein Serine-Threonine Kinases