Abstract
We have identified two new basic domain helix-loop-helix (bHLH) genes in Drosophila melanogaster, DroSCL and DroNHLH. DroSCL was identified because of its homology to the mammalian hematopoietic transcription factor SCL. DroNHLH was similarly identified by homology to NHLH1 and NHLH2, two bHLH genes expressed in the developing mammalian nervous system. A partial DroSCL complementary DNA clone was obtained from an early pupal (5.5-7.5-day) Drosophila library. DroSCL is 73% identical to SCL within the 55-amino acid region of the bHLH domain. A DroNHLH complementary DNA clone was obtained from an early instar (I and II) Drosophila library. Its coding region consists of 162 amino acids and encodes a predicted protein of 18,312 daltons. DroNHLH is 87% identical to NHLH1 and NHLH2 within the bHLH domain. DroSCL and DroNHLH are located on the X chromosome. A 1.7-kilobase DroSCL transcript and a 1.5-kilobase DroNHLH transcript were detected by Northern analysis of total Drosophila RNA. Examination of Drosophila embryos by tissue in situ hybridization reveals restricted expression of both genes in a subset of cells in the developing central nervous system.
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Basic Helix-Loop-Helix Transcription Factors
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Central Nervous System / metabolism
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Chromosome Mapping
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DNA, Complementary
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DNA-Binding Proteins / chemistry
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DNA-Binding Proteins / genetics*
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Drosophila Proteins*
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Drosophila melanogaster / cytology
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Drosophila melanogaster / embryology
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Drosophila melanogaster / genetics*
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Gene Expression Regulation / physiology
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Genes, Insect / genetics*
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Genomic Library
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Helix-Loop-Helix Motifs / genetics
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Humans
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In Situ Hybridization
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Mice
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Molecular Sequence Data
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Nerve Tissue Proteins / chemistry
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Nerve Tissue Proteins / genetics*
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Proto-Oncogene Proteins*
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Sequence Analysis, DNA
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Sequence Homology, Nucleic Acid
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T-Cell Acute Lymphocytic Leukemia Protein 1
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Transcription Factors / chemistry
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Transcription Factors / genetics*
Substances
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Basic Helix-Loop-Helix Transcription Factors
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DNA, Complementary
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DNA-Binding Proteins
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Drosophila Proteins
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HLH3B protein, Drosophila
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HLH4C protein, Drosophila
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Nerve Tissue Proteins
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Proto-Oncogene Proteins
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T-Cell Acute Lymphocytic Leukemia Protein 1
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Tal1 protein, mouse
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Transcription Factors
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TAL1 protein, human
Associated data
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GENBANK/L04924
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GENBANK/L20770
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GENBANK/L20771
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GENBANK/Z25423
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GENBANK/Z25424
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GENBANK/Z25425
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GENBANK/Z25426
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GENBANK/Z25427
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GENBANK/Z25428
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GENBANK/Z25429
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GENBANK/Z25430