Abstract
A human homologue of the bacterial DNAJ heatshock protein, HDJ-2, was isolated from a human umbilical vein endothelial cDNA library using a monoclonal antibody which reacts specifically to human endothelial cells and monocytes. This cDNA clone consists of 1469 nucleotides with an open reading frame of 1191 nucleotides. HDJ-2 shares significant homology with Escherichia coli heat shock protein DNAJ, as well as the yeast homologues Sec63, YDJ1, SCJ1 and SIS1. This homology suggests HDJ-2 may be involved in protein folding and/or transport.
Publication types
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Comparative Study
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Bacterial Proteins / chemistry
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Bacterial Proteins / genetics*
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Base Sequence
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Carrier Proteins*
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Cloning, Molecular
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Consensus Sequence
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Endothelium, Vascular / chemistry*
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Escherichia coli / genetics*
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Escherichia coli Proteins
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HSP40 Heat-Shock Proteins
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Heat-Shock Proteins / chemistry
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Heat-Shock Proteins / genetics*
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Heat-Shock Proteins / isolation & purification
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Humans
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Molecular Sequence Data
Substances
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Bacterial Proteins
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Carrier Proteins
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DNAJA1 protein, human
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DnaJ protein, E coli
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Escherichia coli Proteins
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HSP40 Heat-Shock Proteins
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Heat-Shock Proteins
Associated data
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GENBANK/L08069
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GENBANK/M89789
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GENBANK/S59910
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GENBANK/U00659
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GENBANK/U00663
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GENBANK/U00664
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GENBANK/U00665
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GENBANK/U00666
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GENBANK/U00667
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GENBANK/U00668