Abstract
Major histocompatibility complex (MHC) class I molecules present peptides derived from nuclear and cytosolic proteins to CD8+ T cells. These peptides are translocated into the lumen of the endoplasmic reticulum (ER) to associate with class I molecules. Two MHC-encoded putative transporter proteins, TAP1 and TAP2, are required for efficient assembly of class I molecules and presentation of endogenous peptides. Expression of TAP1 and TAP2 in a mutant cell line resulted in the delivery of an 11-amino acid oligomer model peptide to the ER. Peptide translocation depended on the sequence of the peptide, was adenosine triphosphate (ATP)-dependent, required ATP hydrolysis, and was inhibited in a concentration-dependent manner.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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ATP Binding Cassette Transporter, Subfamily B, Member 2
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ATP Binding Cassette Transporter, Subfamily B, Member 3
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ATP-Binding Cassette Transporters*
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Adenosine Triphosphate / metabolism*
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Amino Acid Sequence
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Animals
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Biological Transport
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Carrier Proteins / metabolism*
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Cell Line
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Cell Membrane Permeability
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Endoplasmic Reticulum / metabolism
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Glycosylation
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Histocompatibility Antigens Class II / metabolism*
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Molecular Sequence Data
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Oligopeptides / metabolism*
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Rats
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T-Lymphocytes, Cytotoxic / metabolism*
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Transfection
Substances
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ATP Binding Cassette Transporter, Subfamily B, Member 2
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ATP Binding Cassette Transporter, Subfamily B, Member 3
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ATP-Binding Cassette Transporters
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Carrier Proteins
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Histocompatibility Antigens Class II
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Oligopeptides
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TAP1 protein, human
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Tap1 protein, rat
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Tap2 protein, rat
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TAP2 protein, human
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Adenosine Triphosphate