PXD017223

PXD017223 is an original dataset announced via ProteomeXchange.

Title	Global proteomics proteasome mutants
Description	Temperature sensitive(TS)missense mutants have been foundational in the characterization of the function of essential genes due to their pronounced cellular phenotypes that are likely associated with protein functional disruption. However, an unbiased approach for the analysis of the biochemical and biophysical changes in missense mutants within the context of their functional proteomes is lacking. We applied mass spectrometry (MS) based thermal proteome profiling (TPP)to investigate the proteome-wide effects of missense mutations in a specific application of TPP that we refer to as mutant Thermal Proteome Profiling (mTPP). This study characterized the global changes in mRNA abundance, protein abundance, and protein thermal stability as a result of missense mutants within two subunits of the yeast ubiquitin-proteasome system. Global protein abundance measurements and RNA sequencing data resulted in a large number of possible candidates that could be causing the phenotypic changes observed in the mutant strains. The additional information gained from mTPP along with complementary proteomic and transcriptomic experiments allows for multiomic intersection analysis that may reveal interesting regulatory categories to pursue in follow-up mechanistic experiments.
HostingRepository	PRIDE
AnnounceDate	2020-11-03
AnnouncementXML	Submission_2020-11-02_23:45:16.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD017223
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Sarah Peck Justice
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2020-01-21 01:35:01	ID requested
⏵ 1	2020-11-02 23:45:16	announced

Peck Justice SA, Barron MP, Qi GD, Wijeratne HRS, Victorino JF, Simpson ER, Vilseck JZ, Wijeratne AB, Mosley AL, Mutant thermal proteome profiling for characterization of missense protein variants and their associated phenotypes within the proteome. J Biol Chem, 295(48):16219-16238(2020) [pubmed]

submitter keyword: Yeast, proteasome, TMT, TPP

Amber L Mosley
contact affiliation	Department of Biochemistry and Molecular Biology Indiana University School of Medicine Indianapolis, IN
contact email	almosley@iu.edu
lab head
Sarah Peck Justice
contact affiliation	Indiana University School of Medicine
contact email	sapeck@iu.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD017223
     1. Label: PRIDE project
     2. Name: Global proteomics proteasome mutants