PXD021676

PXD021676 is an original dataset announced via ProteomeXchange.

Title	T7 DNA Polymerase with fluorescent unnatural amino acid
Description	A T7 DNA polymerase variant with the fluorescent unnatural amino acid (7-hydroxycoumarin-4-yl) ethylglycine (7-HCou) incorporated at position 514 was expressed in E. coli and purified. The protein was then characterized by mass spec, pre-steady state kinetic experiments, and fluorescence measurements.
HostingRepository	PRIDE
AnnounceDate	2020-10-19
AnnouncementXML	Submission_2020-10-19_00:57:50.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD021676
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Tyler Dangerfield
SpeciesList	scientific name: Escherichia virus T7; NCBI TaxID: 1985738;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2020-09-24 22:43:59	ID requested
⏵ 1	2020-10-19 00:57:50	announced

Dangerfield TL, Johnson KA, Optimized incorporation of an unnatural fluorescent amino acid affords measurement of conformational dynamics governing high-fidelity DNA replication. J Biol Chem, 295(50):17265-17280(2020) [pubmed]

submitter keyword: T7, DNA polymerase, Unnatural amino acid, 7-hydroxycoumarin-4-yl ethylglycine

Kenneth A. Johnson
contact affiliation	Molecular Biosciences, University of Texas at Austin, USA
contact email	kajohnson@mail.utexas.edu
lab head
Tyler Dangerfield
contact affiliation	University of Texas at Austin
contact email	tylerdangerfield@utexas.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/10/PXD021676

PRIDE project URI

• PRIDE
  1. PXD021676
     1. Label: PRIDE project
     2. Name: T7 DNA Polymerase with fluorescent unnatural amino acid