PXD017717

PXD017717 is an original dataset announced via ProteomeXchange.

Title	Protein S-glutathionylation in HeLa cells identified by nanoLC-LTQ-Orbitrap XL MS/MS
Description	Cysteine modifications have been widely detected among different protein in different species. Here, we examined protein glutathionylation in HeLa cells under both untreated and diamide-treated conditions. A strategy using resin-assisted enrichment of glutathionylated proteins or peptides after biotin-switch is commonly used for detection of glutathionylation and other reversible cysteine modifications, and was also applied in this study. Using these strategies we identified a large number of glutathionylated protein with modification sites under both untreated and diamide-treated conditions.
HostingRepository	PRIDE
AnnounceDate	2020-04-29
AnnouncementXML	Submission_2020-04-28_22:06:17.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Hong Zhang
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	EDT-maleimide-PEO-biotin
Instrument	LTQ Orbitrap XL

Revision	Datetime	Status	ChangeLog Entry
0	2020-02-25 06:32:20	ID requested
⏵ 1	2020-04-28 22:06:18	announced

Yang J, Zhang H, Gong W, Liu Z, Wu H, Hu W, Chen X, Wang L, Wu S, Chen C, Perrett S, -helical lid. J Biol Chem, 295(24):8302-8324(2020) [pubmed]

submitter keyword: S-glutathionylation, nanoLC-LTQ-Orbitrap XL MS/MS, biotin switch

Sarah Perrett
contact affiliation	Institute of Biophysics, Chinese Academy of Sciences
contact email	sperrett@ibp.ac.cn
lab head
Hong Zhang
contact affiliation	Institute of Biophysics， Chinese Academy of Sciences
contact email	zhangh@moon.ibp.ac.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/04/PXD017717

PRIDE project URI

• PRIDE
  1. PXD017717
     1. Label: PRIDE project
     2. Name: Protein S-glutathionylation in HeLa cells identified by nanoLC-LTQ-Orbitrap XL MS/MS