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PXD018745

PXD018745 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleA novel transcriptional signalling pathway mediated by the trafficking protein Ambra1 via scaffolding Atf2 complexes
DescriptionPreviously, we reported that Ambra1 is a core component of a cytoplasmic trafficking network, acting as a spatial rheostat to control active Src and FAK levels in addition to its critical roles in autophagy during neurogenesis. Here we identify a novel nuclear scaffolding function for Ambra1 that controls gene expression. Ambra1 binds to nuclear pore proteins, to other adaptor proteins like FAK and Akap8 in the nucleus, as well as to chromatin modifiers and transcriptional regulators such as Brg1, Cdk9 and the cAMP-regulated transcription factor Atf2. Ambra1 contributes to their association with chromatin and we identified genes whose transcription is regulated by Ambra1 complexes, likely via histone modifications and phospho-Atf2-dependent transcription. Therefore, Ambra1 scaffolds protein complexes at chromatin, regulating transcriptional signalling in the nucleus; in particular, it recruits chromatin modifiers and transcriptional regulators to control expression of genes such as Angpt1, Tgfb2, Tgfb3, Itga8 and Itgb7 that likely contribute to the role of Ambra1 in cancer cell invasion.
HostingRepositoryPRIDE
AnnounceDate2021-09-09
AnnouncementXMLSubmission_2021-09-08_19:05:53.700.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterAlex von kriegsheim
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-04-22 23:50:58ID requested
12021-09-08 19:05:54announced
Publication List
Schoenherr C, Byron A, Griffith B, Loftus A, Wills JC, Munro AF, von Kriegsheim A, Frame MC, The autophagy protein Ambra1 regulates gene expression by supporting novel transcriptional complexes. J Biol Chem, 295(34):12045-12057(2020) [pubmed]
Keyword List
submitter keyword: Interaction proteomics, signalling,
Contact List
Alex von kriegsheim
contact affiliationUniversity of Edinburgh
contact emailalex.vonrkriegheim@igmm.ed.ac.uk
lab head
Alex von kriegsheim
contact affiliationUniversity of Edinburgh
contact emailalex.vonkriegsheim@ucd.ie
dataset submitter
Full Dataset Link List
Dataset FTP location
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