CAT5 / YOR125C Overview


Standard Name
CAT5 1
Systematic Name
YOR125C
SGD ID
SGD:S000005651
Aliases
COQ7 4
Feature Type
ORF , Verified
Description
Protein required for ubiquinone (Coenzyme Q) biosynthesis; localizes to the matrix face of the mitochondrial inner membrane in a large complex with ubiquinone biosynthetic enzymes; required for gluconeogenic gene activation 3 4 5 6
Name Description
CATabolite repression 2
Comparative Info
Sequence Details

Sequence

The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.


Summary
CAT5 is located on the right arm of chroosome XV between UBP2 ubiquitin-specific protease and IAH1 isoamyl acetate-hydrolyzing esterase; coding sequence is 702 nucleotides long with 4 SNPs, 1 of which leads to an amino acid polymorphism in the ferritin-like domain
Protein Details

Protein

Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.


Summary
Cat5p is 233 amino acids long, phosphorylated on 4 residues, Ile/Met polymorphism at amino acid 91 within ferritin-like domain, protein abundance increases under nitrogen limitation
Length (a.a.)
233
Mol. Weight (Da)
26061.0
Isoelectric Point
8.14
Median Abundance (molecules/cell)
2298 +/- 1164
Half-life (hr)
8.1

Alleles

Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results. Click "YeastMine" to view all alleles in YeastMine.


View all CAT5 alleles in SGD search | YeastMine

Gene Ontology Details

Gene Ontology

GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.


Summary
Mitochondrial inner membrane protein predicted to have monoxygenase activity; involved in ubiquinone biosynthetic process

View computational annotations

Molecular Function

Manually Curated

Biological Process

Manually Curated

Cellular Component

Manually Curated

Complex

Macromolecular complex annotations are imported from the Complex Portal. These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background.


Pathways


Phenotype Details

Phenotype

Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.


Summary
Non-essential gene; null mutant is incapable of respiratory growth on non-fermentable carbon sources; null mutant is also sensitive to oxidative stress and linoleic acid; in systematic studies null mutation results in reduced competitive fitness
Disease Details

Disease

Disease Annotations consist of three mandatory components: a gene product, a term from the Disease Ontology (DO) controlled vocabulary and an evidence code. SGD provides manually curated DO Annotations derived from the literature. Click "Disease Details" to view all Disease information and evidence for this locus as well as diseases it shares with other genes.


Summary
Yeast CAT5 is homologous to human COQ7 and has been used to study mutations found in patients with primary coenzyme Q10 deficiency 8
Interaction Details

Interaction

Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.


Summary
Cat5p interacts physically with proteins involved in translation regulation and RNA catabolism; CAT5 interacts genetically with genes involved in transcription

491 total interactions for 424 unique genes

Physical Interactions

  • Affinity Capture-MS: 41
  • Affinity Capture-RNA: 8
  • Affinity Capture-Western: 5
  • Biochemical Activity: 2
  • Co-fractionation: 2
  • Co-purification: 2
  • PCA: 1
  • Protein-RNA: 1
  • Reconstituted Complex: 1

Genetic Interactions

  • Dosage Rescue: 2
  • Negative Genetic: 370
  • Phenotypic Enhancement: 2
  • Phenotypic Suppression: 2
  • Positive Genetic: 42
  • Synthetic Growth Defect: 9
  • Synthetic Rescue: 1
Regulation Details

Regulation

The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.


Summary
Transcription of CAT5 is regulated by Ixr1p, upregulated by Hcm1p, and regulated by Sfp1p in reponse to stress; promoter bound by Rgr1p in response to heat
Regulators
4
Targets
0
Expression Details

Expression

Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.


Literature Details

Literature

All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.


Primary
37
Additional
38
Reviews
25

Resources