(Peter Espenshade/Johns Hopkins University School of Medicine Cat# 4845, RRID:AB_2713965)Copy Citation Copied
URL: http://antibodyregistry.org/AB_2713965
Proper Citation: (Peter Espenshade/Johns Hopkins University School of Medicine Cat# 4845, RRID:AB_2713965)
Target Antigen: Sre1
Host Organism: rabbit
Clonality: polyclonal
Comments: "Polyclonal antibody against amino acids 1-260 of Sre1 was generated by immunizing rabbits with bacterially expressed antigen using a standard protocol (Harlow and Lane, 1999). Antigen containing an Nterminal polyhistidine tag and a tobacco etch virus (TEV) protease cleavage sequence was purified from E. coli using Ni-NTA agarose (Qiagen) and then cleaved to remove the histidine tag using TEV protease (Invitrogen) according to manufacturer’s instructions. Sre1-specific antibodies were isolated from rabbit serum by affinity chromatography using NHS-Sepharose resin (Pierce) conjugated to the polyhistidine tagged Sre1 antigen (Harlow and Lane, 1999)." - PMID:15797383
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