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Modelling of fluid-phase endocytosis kinetics in the amoebae of the cellular slime mouldDictyostelium discoideum. A multicompartmental approach

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Abstract

Fluid-phase endocytosis (pinocytosis) kinetics were studied inDictyostelium discoideum amoebae from the axenic strain Ax-2 that exhibits high rates of fluid-phase endocytosis when cultured in liquid nutrient media. Fluorescein-labelled dextran (FITC-dextran) was used as a marker in continuous uptake- and in pulse-chase exocytosis experiments. In the latter case, efflux of the marker was monitored on cells loaded for short periods of time and resuspended in marker-free medium. A multicompartmental model was developed which describes satisfactorily fluid-phase endocytosis kinetics. In particular, it accounts correctly for the extended latency period before exocytosis in pulse-chase experiments and it suggests the existence of some sorts of maturation stages in the pathway.

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Authors and Affiliations

  1. Laboratoire de Biologie Cellulaire, URA 1130 CNRS, CEA, Département de Biologie Moléculaire et Structurale, Centre d'Etudes Nucléaires, 17, rue des Martyrs, 38054, Grenoble Cedex 9, France

    Laurence Aubry, Gérard Klein & Michel Satre

  2. Laboratoire de Techniques d'Imagerie, de Modélisation et de Cognition (TIMC), URA 1618 CNRS, Département de Mathématiques, Faculté de Médecine, 38700, La Tronche, France

    Jean-Louis Martiel

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  1. Laurence Aubry
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  2. Gérard Klein
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  3. Jean-Louis Martiel
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  4. Michel Satre
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Aubry, L., Klein, G., Martiel, JL. et al. Modelling of fluid-phase endocytosis kinetics in the amoebae of the cellular slime mouldDictyostelium discoideum. A multicompartmental approach. Acta Biotheor 43, 319–333 (1995). https://doi.org/10.1007/BF00713556

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