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| Status |
Public on Jun 30, 2014 |
| Title |
Genome-wide profiling of altered gene expression in the neocortex of Alzheimer's disease (exon level) |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
We investigated genome-wide gene alterations in the temporal cortex of a well-characterized cohort of Alzheimer’s disease (AD) patients using Affymetrix exon arrays.
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| Overall design |
The AD patients and controls are subjects originally recruited into OPTIMA (Oxford Project to Investigate Memory and Ageing). Subjects underwent annual clinical and neuropsychological assessments, including the Cambridge Cognitive Examination (CAMCOG). Among subjects who died with autopsy consent given by next of kin, 1 cm3 blocks of grey matter from BA22 were dissected from fresh frozen brains of AD subjects. Tissues from elderly controls, followed longitudinally in life and with CAMCOG scores >80 at the last assessment before death, were collected. Affymetrix exon array was carried out in 8 control and 8 AD samples. The samples processed in each hybridization run were matched for AD vs. control, sex and postmortem interval (PMI) as far as possible.
This submission includes the exon-level analysis.
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| Contributor(s) |
Tan MG, Lai MK |
| Citation |
Mitchell K.P. Lai, Margaret M. Esiri, Michelle G.K. Tan. Genome-wide profiling of alternative splicing in Alzheimer's disease. Genomics Data 2 (2014) 290-292. DOI: http://dx.doi.org/10.1016/j.gdata.2014.09.002
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| Submission date |
Apr 13, 2012 |
| Last update date |
Sep 30, 2014 |
| Contact name |
Michelle GK Tan |
| E-mail(s) |
michelle.tan.g.k@sgh.com.sg
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| Organization name |
Singapore General Hospital
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| Department |
Clinical Translational Research
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| Street address |
20 College Road
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| City |
Singapore |
| ZIP/Postal code |
169856 |
| Country |
Singapore |
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| Platforms (1) |
| GPL5188 |
[HuEx-1_0-st] Affymetrix Human Exon 1.0 ST Array [probe set (exon) version] |
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| Samples (16)
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| Relations |
| BioProject |
PRJNA159155 |
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