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| Status |
Public on Sep 10, 2018 |
| Title |
Combined RNA-seq and RAT-seq mapping of long noncoding RNAs in pluripotent reprogramming |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
RNA transcriptome sequencing (RNA-Seq) and RNA reverse transcription-associated trap sequencing (RAT-Seq) were usded to map long noncoding RNA (lncRNAs) in reprogramming of fibroblasts into pluripotency
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| Overall design |
Fibroblasts (FIB) and induced pluripotent stem cells (iPSCs) were collected in the process of reprogramming. RNA-Seq was used to identify lncRNAs that are differentially-expressed in reprogramming. RAT-Seq was utilized to generate the database to map the regulatory element network of lncRNA candidate Osclr8 (NONMMUT043505, Platr10).
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| Contributor(s) |
Du Z, Wen X, Li W, Cui J, Hu J |
| Citation(s) |
30457566, 31903125, 32055844, 32398955, 34412677 |
| Submission date |
Jul 03, 2018 |
| Last update date |
Aug 24, 2021 |
| Contact name |
Jifan Hu |
| E-mail(s) |
jifan@stanford.edu
|
| Phone |
6508523175
|
| Organization name |
Stanford University Medical School
|
| Department |
Palo Alto Veterans Institute for Research
|
| Lab |
Bldg 100, Room E4-200
|
| Street address |
3801 Miranda Ave, Bldg 100, Room E4-200
|
| City |
Palo Alto |
| State/province |
CA |
| ZIP/Postal code |
94304 |
| Country |
USA |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA479482 |
| SRA |
SRP151932 |
