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| Status |
Public on Feb 26, 2021 |
| Title |
Genome-wide analysis of the CARD9/Dectin-1 transcriptional program in neutrophils and macrophages by RNA-seq |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Purpose: To characterize Card9-dependent and Card9-independent Dectin-1 mediated gene expression changes in neutrophils and to compare response to Dectin-1 stimulation in neutrophils and macrophages.
Method: BM neutrophils from WT and Card9-/- mice were isolated by MACS column purification and treated with or without the Dectin-1 agonist, curdlan for 3 hrs. WT BM-derived macrophages were also treated with or without curdlan for 3hrs before sample collection.
Results: We identified both Card9-dependent and Card9-independent gene expression changes with Dectin-1 stimulation in neutrophils. We also identified cell-type specific differences in the response to Dectin-1 stimulation in neutrophils and macrophages.
Conclusions: Dectin-1 stimulation induces significant gene expression changes regulated by either canonical Card9-dependent or non-canonical Card9-independent mechanisms.
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| Overall design |
WT and Card9-deficient neutrophils +/- curdlan (n=3/group); WT macrophages +/- curdlan (n=2/group)
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| |
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| Contributor(s) |
Deerhake ME, Shinohara ML |
| Citation(s) |
33581044 |
| Submission date |
Apr 17, 2020 |
| Last update date |
Feb 27, 2021 |
| Contact name |
Mari L Shinohara |
| E-mail(s) |
ms294@duke.edu
|
| Phone |
9196136977
|
| Organization name |
Duke University School of Medicine
|
| Department |
Immunology
|
| Lab |
Shinohara
|
| Street address |
DUMC-3010
|
| City |
Durham |
| State/province |
NC |
| ZIP/Postal code |
27710 |
| Country |
USA |
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| Platforms (1) |
| GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
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| Samples (16)
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| Relations |
| BioProject |
PRJNA625958 |
| SRA |
SRP256919 |
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