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| Status |
Public on Apr 13, 2022 |
| Title |
RNA-seq analysis of the effect of loss of Tp53, NF1 or CBS in telomerase-reverse transcriptase (TERT)-immortalized human BJ fibroblasts (BJ-TERT) overexpressing myristoylated AKT1 |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Overexpression myristoylated AKT1 in human BJ-TERT fibroblasts induces senescence (AIS). We depleted CBS, Tp53 or NF1 gene by shRNA in AIS cells and performed RNA-seq analysis to investigate the regulatory effect of these genes in AIS maintenance.
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| Overall design |
BJ-TERT cells were transduced with pBabe-puro empty vector (EV) or pBabe-puro-myrAKT1 to induce senescence. On day 6 post-transduction myrAKT1 cells were transduced with either pGIPZ-shRNA against CBS, Tp53 and NF1, or the control vector pGIPZ-NTC, pBabe-EV cells transduced with pGIPZ-shRNA non-targeting control (NTC). Total RNA was extracted on day 14 post-transduction for RNA-seq. There are five samples including pBabe-NTC, AKT1-NTC, AKT-shTP53, AKT-shNF1, AKT-shCBS with three replicates.
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| Contributor(s) |
Zhu H, Kang J, Chan KT, Pearson RB |
| Citation(s) |
35758651 |
| Submission date |
Apr 08, 2022 |
| Last update date |
Jul 07, 2022 |
| Contact name |
Jian Kang |
| E-mail(s) |
jkang@svi.edu.au
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| Organization name |
St Vincent's institute of medical research
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| Department |
research division
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| Lab |
DNA damage and cancer therapy lab
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| Street address |
9 Princes street, Fitzroy
|
| City |
Melbourne |
| State/province |
Victoria |
| ZIP/Postal code |
3065 |
| Country |
Australia |
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| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
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| Samples (15)
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| Relations |
| BioProject |
PRJNA824798 |
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