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Record Information

Version

5.0

Status

Detected and Quantified

Creation Date

2006-08-12 19:53:05 UTC

Update Date

2020-05-07 18:05:00 UTC

HMDB ID

HMDB0003378

Secondary Accession Numbers

HMDB0032439
HMDB03378
HMDB32439

Metabolite Identification

Common Name

Nitric oxide

Description

The biologically active molecule nitric oxide (NO) is a simple, membrane-permeable gas with unique chemistry. It is formed by the conversion of L-arginine to L-citrulline, with the release of NO. The enzymatic oxidation of L-arginine to L-citrulline takes place in the presence of oxygen and NADPH using flavin adenine dinucleotide (FAD), flavin mononucleotide (FMN), heme, thiol, and tetrahydrobiopterin as cofactors. The enzyme responsible for the generation of NO is nitric oxide synthase (E.C. 1.7.99.7; NOS). Three NOS isoforms have been described and shown to be encoded on three distinct genes: neuronal NOS (nNOS, NOS type I), inducible NOS (NOS type II), and endothelial NOS (eNOS, NOS type III). Two of them are constitutively expressed and dependent on the presence of calcium ions and calmodulin to function (nNOS and eNOS), while iNOS is considered non-constitutive and calcium-independent. However, experience has shown that constitutive expression of nNOS and eNOS is not as rigid as previously thought (i.e. either present or absent), but can be dynamically controlled during development and in response to injury. Functionally, NO may act as a hormone, neurotransmitter, paracrine messenger, mediator, cytoprotective molecule, and cytotoxic molecule. NO has multiple cellular molecular targets. It influences the activity of transcription factors, modulates upstream signaling cascades, mRNA stability and translation, and processes the primary gene products. In the brain, many processes are linked to NO. NO activates its receptor, soluble guanylate cyclase by binding to it. The stimulation of this enzyme leads to increased synthesis of the second messenger, cGMP, which in turn activates cGMP-dependent kinases in target cells. NO exerts a strong influence on glutamatergic neurotransmission by directly interacting with the N-methyl-D-aspartate (NMDA) receptor. Neuronal NOS is connected to NMDA receptors (see below) and sharply increases NO production following activation of this receptor. Thus, the level of endogenously produced NO around NMDA synapses reflects the activity of glutamate-mediated neurotransmission. However, there is recent evidence showing that non-NMDA glutamate receptors (i.e. AMPA and type I metabotropic receptors) also contribute to NO generation. Besides its influence on glutamate, NO is known to have effects on the storage, uptake and/or release of most other neurotransmitters in the CNS (acetylcholine, dopamine, noradrenaline, GABA, taurine, and glycine) as well as of certain neuropeptides. Finally, since NO is a highly diffusible molecule, it may reach extrasynaptic receptors at target cell membranes that are some distance away from the place of NO synthesis. NO is thus capable of mediating both synaptic and nonsynaptic communication processes. NO is a potent vasodilator (a major endogenous regulator of vascular tone), and an important endothelium-dependent relaxing factor. NO is synthesized by NO synthases (NOS) and NOS are inhibited by asymmetrical dimethylarginine (ADMA). ADMA is metabolized by dimethylarginine dimethylaminohydrolase (DDAH) and excreted in the kidneys. Lower ADMA levels in pregnant women compared to non-pregnant controls suggest that ADMA has a role in vascular dilatation and blood pressure changes. Several studies show an increase in ADMA levels in pregnancies complicated with preeclampsia. Elevated ADMA levels in preeclampsia are seen before clinical symptoms have developed; these findings suggest that ADMA has a role in the pathogenesis of preeclampsia. In some pulmonary hypertensive states such as ARDS, the production of endogenous NO may be impaired. Nitric oxide inhalation selectively dilates the pulmonary circulation. Significant systemic vasodilation does not occur because NO is inactivated by rapidly binding to hemoglobin. In an injured lung with pulmonary hypertension, inhaled NO produces local vasodilation of well-ventilated lung units and may "steal" blood flow away from unventilated regions. This reduces intrapulmonary shunting and may improve systemic arterial oxygenation. Nitric oxide is a chemical mediator fundamental in the maintenance of adequate tissue perfusion and effective cardiovascular function. The use of nitrates is well established as pharmacological agents but it is only recently that it has been recognized that they act as a source of nitric oxide (PMID: 16966108 , 8752507 , 17181668 , 16005189 ). Nitric oxide is used as a food additive (EAFUS: Everything Added to Food in the United States).

Structure

MOL 3D MOL SDF 3D SDF PDB 3D PDB SMILES InChI

View in JSmol View Stereo Labels

Synonyms

Value	Source
(NO)(.)	ChEBI
[NO]	ChEBI
EDRF	ChEBI
Endothelium-derived relaxing factor	ChEBI
Mononitrogen monoxide	ChEBI
Monoxido de nitrogeno	ChEBI
Monoxyde d'azote	ChEBI
Nitrogen monooxide	ChEBI
Nitrogen monoxide	ChEBI
Nitrosyl	ChEBI
NO	ChEBI
(.)NO	ChEBI
NO(.)	ChEBI
Oxido de nitrogeno(II)	ChEBI
Oxido nitrico	ChEBI
Oxyde azotique	ChEBI
Oxyde nitrique	ChEBI
Stickstoff(II)-oxid	ChEBI
Stickstoffmonoxid	ChEBI
INOmax	Kegg
Nitrogen oxide	HMDB
Nitrogen protoxide	HMDB
Nitrosyl hydride	HMDB
Nitrosyl radical	HMDB
Nitroxide radical	HMDB
Nitroxyl	HMDB
Endogenous nitrate vasodilator	MeSH, HMDB
Monoxide, nitrogen	MeSH, HMDB
Nitric oxide, endothelium derived	MeSH, HMDB
Nitric oxide, endothelium-derived	MeSH, HMDB
Monoxide, mononitrogen	MeSH, HMDB
Vasodilator, endogenous nitrate	MeSH, HMDB
Endothelium-derived nitric oxide	MeSH, HMDB
Nitrate vasodilator, endogenous	MeSH, HMDB
Oxide, nitric	MeSH, HMDB

Chemical Formula

Average Molecular Weight

30.0061

Monoisotopic Molecular Weight

29.997988627

IUPAC Name

nitroso

Traditional Name

nitric oxide

CAS Registry Number

10102-43-9

SMILES

[N]=O

InChI Identifier

InChI=1S/NO/c1-2

InChI Key

MWUXSHHQAYIFBG-UHFFFAOYSA-N

Chemical Taxonomy

Description

Belongs to the class of inorganic compounds known as other non-metal oxides. These are inorganic compounds containing an oxygen atom of an oxidation state of -2, in which the heaviest atom bonded to the oxygen belongs to the class of 'other non-metals'.

Kingdom

Inorganic compounds

Super Class

Homogeneous non-metal compounds

Class

Other non-metal organides

Sub Class

Other non-metal oxides

Direct Parent

Other non-metal oxides

Alternative Parents

Inorganic oxides

Substituents

Other non-metal oxide
Inorganic oxide

Molecular Framework

Not Available

External Descriptors

reactive nitrogen species (CHEBI:16480 )
reactive oxygen species (CHEBI:16480 )
nitrogen oxide (CHEBI:16480 )
inorganic radical (CHEBI:16480 )

Ontology

Not Available

Exogenous (HMDB: HMDB0032439)

Endogenous

Endogenous (HMDB: HMDB0032439)

Process

Not Available

Role

Not Available

Physical Properties

State

Liquid

Experimental Molecular Properties

Property	Value	Reference
Melting Point	-163.6 °C	Not Available
Boiling Point	Not Available	Not Available
Water Solubility	Not Available	Not Available
LogP	Not Available	Not Available

Experimental Chromatographic Properties

Not Available

Predicted Molecular Properties

Property	Value	Source
logP	-0.35	ChemAxon
pKa (Strongest Basic)	-2.9	ChemAxon
Physiological Charge	0	ChemAxon
Hydrogen Acceptor Count	2	ChemAxon
Hydrogen Donor Count	0	ChemAxon
Polar Surface Area	34.14 Å²	ChemAxon
Rotatable Bond Count	0	ChemAxon
Refractivity	2.89 m³·mol⁻¹	ChemAxon
Polarizability	1.69 Å³	ChemAxon
Number of Rings	0	ChemAxon
Bioavailability	Yes	ChemAxon
Rule of Five	Yes	ChemAxon
Ghose Filter	No	ChemAxon
Veber's Rule	Yes	ChemAxon
MDDR-like Rule	No	ChemAxon

Predicted Chromatographic Properties

Predicted Collision Cross Sections

Predictor	Adduct Type	CCS Value (Å²)	Reference
DeepCCS	[M+H]+	110.435	30932474
DeepCCS	[M-H]-	108.681	30932474
DeepCCS	[M-2H]-	143.856	30932474
DeepCCS	[M+Na]+	117.187	30932474

Predicted Kovats Retention Indices

Underivatized

Metabolite	SMILES	Kovats RI Value	Column Type	Reference
Nitric oxide	[N]=O	713.9	Standard polar	33892256
Nitric oxide	[N]=O	279.7	Standard non polar	33892256
Nitric oxide	[N]=O	345.9	Semi standard non polar	33892256

Spectra

GC-MS Spectra

Spectrum Type	Description	Splash Key	Deposition Date	Source	View
Predicted GC-MS	Predicted GC-MS Spectrum - Nitric oxide GC-MS (Non-derivatized) - 70eV, Positive	splash10-001i-9000000000-d182bfca83d886821e6d	2016-09-22	Wishart Lab	View Spectrum
Predicted GC-MS	Predicted GC-MS Spectrum - Nitric oxide GC-MS (Non-derivatized) - 70eV, Positive	Not Available	2021-10-12	Wishart Lab	View Spectrum

MS/MS Spectra

Spectrum Type	Description	Splash Key	Deposition Date	Source	View
Predicted LC-MS/MS	Predicted LC-MS/MS Spectrum - Nitric oxide 10V, Positive-QTOF	splash10-001i-9000000000-8a2dd9da1fc114d013b3	2015-09-15	Wishart Lab	View Spectrum
Predicted LC-MS/MS	Predicted LC-MS/MS Spectrum - Nitric oxide 20V, Positive-QTOF	splash10-001i-9000000000-8a2dd9da1fc114d013b3	2015-09-15	Wishart Lab	View Spectrum
Predicted LC-MS/MS	Predicted LC-MS/MS Spectrum - Nitric oxide 40V, Positive-QTOF	splash10-001i-9000000000-8a2dd9da1fc114d013b3	2015-09-15	Wishart Lab	View Spectrum
Predicted LC-MS/MS	Predicted LC-MS/MS Spectrum - Nitric oxide 10V, Negative-QTOF	splash10-004i-9000000000-e998110984a05853a05d	2015-09-15	Wishart Lab	View Spectrum
Predicted LC-MS/MS	Predicted LC-MS/MS Spectrum - Nitric oxide 20V, Negative-QTOF	splash10-004i-9000000000-e998110984a05853a05d	2015-09-15	Wishart Lab	View Spectrum
Predicted LC-MS/MS	Predicted LC-MS/MS Spectrum - Nitric oxide 40V, Negative-QTOF	splash10-004i-9000000000-e998110984a05853a05d	2015-09-15	Wishart Lab	View Spectrum
Predicted LC-MS/MS	Predicted LC-MS/MS Spectrum - Nitric oxide 10V, Positive-QTOF	splash10-001i-9000000000-4faa0e3c79683567bab0	2021-09-23	Wishart Lab	View Spectrum
Predicted LC-MS/MS	Predicted LC-MS/MS Spectrum - Nitric oxide 20V, Positive-QTOF	splash10-001i-9000000000-4faa0e3c79683567bab0	2021-09-23	Wishart Lab	View Spectrum
Predicted LC-MS/MS	Predicted LC-MS/MS Spectrum - Nitric oxide 40V, Positive-QTOF	splash10-001i-9000000000-4faa0e3c79683567bab0	2021-09-23	Wishart Lab	View Spectrum

Biological Properties

Cellular Locations

Cytoplasm
Extracellular

Biospecimen Locations

Blood
Cerebrospinal Fluid (CSF)

Tissue Locations

Adipose Tissue
Adrenal Gland
Adrenal Medulla
Bladder
Brain
Epidermis
Fibroblasts
Intestine
Kidney
Lung
Neuron
Pancreas
Placenta
Platelet
Prostate
Skeletal Muscle
Spleen
Testis
Thyroid Gland

Pathways

Name	SMPDB/PathBank	KEGG
Arginine and proline metabolism	Not Available
Prolidase Deficiency (PD)		Not Available
Arginine: Glycine Amidinotransferase Deficiency (AGAT Deficiency)		Not Available
Hyperprolinemia Type II		Not Available
Hyperprolinemia Type I		Not Available

Normal Concentrations

Biospecimen	Status	Value	Age	Sex	Condition	Reference	Details
Blood	Detected and Quantified	0.000012 +/- 0.000006 uM	Adult (>18 years old)	Both	Normal	17267398	details
Cerebrospinal Fluid (CSF)	Detected and Quantified	1.05 +/- 0.01 uM	Adult (>18 years old)	Male	Normal	18597230	details
Cerebrospinal Fluid (CSF)	Detected and Quantified	8.66 +/- 1.07 uM	Newborn (0-30 days old)	Not Specified	Normal	12585720	details

Abnormal Concentrations

Biospecimen	Status	Value	Age	Sex	Condition	Reference	Details
Cerebrospinal Fluid (CSF)	Detected and Quantified	8.60 +/- 0.49 uM	Newborn (0-30 days old)	Not Specified	Hypoxic-ischemic encephalopathy	12585720	details
Cerebrospinal Fluid (CSF)	Detected and Quantified	0.99 +/- 0.01 uM	Adult (>18 years old)	Male	Subarachnoid hemorrhage	18597230	details

Associated Disorders and Diseases

Disease References

Subarachnoid hemorrhage
Durmaz R, Ozkara E, Kanbak G, Arslan OC, Dokumacioglu A, Kartkaya K, Atasoy MA: Nitric oxide level and adenosine deaminase activity in cerebrospinal fluid of patients with subarachnoid hemorrhage. Turk Neurosurg. 2008 Apr;18(2):157-64. [PubMed:18597230 ]
Hypoxic-ischemic encephalopathy
Gucuyener K, Ergenekon E, Demiryurek T, Erbas D, Ozturk G, Koc E, Atalay Y: Cerebrospinal fluid levels of nitric oxide and nitrotyrosine in neonates with mild hypoxic-ischemic encephalopathy. J Child Neurol. 2002 Nov;17(11):815-8. [PubMed:12585720 ]

Associated OMIM IDs

105800 (Subarachnoid hemorrhage)

External Links

DrugBank ID

DB00435

Phenol Explorer Compound ID

Not Available

FooDB ID

Not Available

KNApSAcK ID

Not Available

Chemspider ID

127983

KEGG Compound ID

C00533

BioCyc ID

NITRIC-OXIDE

BiGG ID

Not Available

Wikipedia Link

Nitric oxide

METLIN ID

Not Available

PubChem Compound

145068

PDB ID

Not Available

ChEBI ID

16480

Food Biomarker Ontology

Not Available

VMH ID

Not Available

MarkerDB ID

Not Available

Good Scents ID

Not Available

References

Synthesis Reference

Morabito, Paul; Heicklen, Julian. Disproportionation to combination ratios of alkoxy radicals with nitric oxide. Journal of Physical Chemistry (1985), 89(13), 2914-16.

Material Safety Data Sheet (MSDS)

Not Available

General References

Kaynar H, Meral M, Turhan H, Keles M, Celik G, Akcay F: Glutathione peroxidase, glutathione-S-transferase, catalase, xanthine oxidase, Cu-Zn superoxide dismutase activities, total glutathione, nitric oxide, and malondialdehyde levels in erythrocytes of patients with small cell and non-small cell lung cancer. Cancer Lett. 2005 Sep 28;227(2):133-9. Epub 2005 Jan 8. [PubMed:16112416 ]
Noris M, Todeschini M, Cassis P, Pasta F, Cappellini A, Bonazzola S, Macconi D, Maucci R, Porrati F, Benigni A, Picciolo C, Remuzzi G: L-arginine depletion in preeclampsia orients nitric oxide synthase toward oxidant species. Hypertension. 2004 Mar;43(3):614-22. Epub 2004 Jan 26. [PubMed:14744923 ]
Moon A: Influence of nitric oxide signalling pathways on pre-contracted human detrusor smooth muscle in vitro. BJU Int. 2002 Jun;89(9):942-9. [PubMed:12010245 ]
Muerkoster S, Wegehenkel K, Arlt A, Witt M, Sipos B, Kruse ML, Sebens T, Kloppel G, Kalthoff H, Folsch UR, Schafer H: Tumor stroma interactions induce chemoresistance in pancreatic ductal carcinoma cells involving increased secretion and paracrine effects of nitric oxide and interleukin-1beta. Cancer Res. 2004 Feb 15;64(4):1331-7. [PubMed:14973050 ]
Huledal G, Jonzon B, Malmenas M, Hedman A, Andersson LI, Odlind B, Brater DC: Renal effects of the cyclooxygenase-inhibiting nitric oxide donator AZD3582 compared with rofecoxib and naproxen during normal and low sodium intake. Clin Pharmacol Ther. 2005 May;77(5):437-50. [PubMed:15900289 ]
Brown DA, Canning MT, Nay SL, Pena AV, Yarosh DB: Bicyclic monoterpene diols stimulate release of nitric oxide from skin cells, increase microcirculation, and elevate skin temperature. Nitric Oxide. 2006 Aug;15(1):70-6. Epub 2006 Apr 19. [PubMed:16626981 ]
Santoro G, Romeo C, Impellizzeri P, Ientile R, Cutroneo G, Trimarchi F, Pedale S, Turiaco N, Gentile C: Nitric oxide synthase patterns in normal and varicocele testis in adolescents. BJU Int. 2001 Dec;88(9):967-73. [PubMed:11851622 ]
Browning DD, McShane MP, Marty C, Ye RD: Nitric oxide activation of p38 mitogen-activated protein kinase in 293T fibroblasts requires cGMP-dependent protein kinase. J Biol Chem. 2000 Jan 28;275(4):2811-6. [PubMed:10644746 ]
Heym C, Colombo-Benckmann M, Mayer B: Immunohistochemical demonstration of the synthesis enzyme for nitric oxide and of comediators in neurons and chromaffin cells of the human adrenal medulla. Ann Anat. 1994 Jan;176(1):11-6. [PubMed:7508210 ]
Neri S, Signorelli S, Pulvirenti D, Mauceri B, Cilio D, Bordonaro F, Abate G, Interlandi D, Misseri M, Ignaccolo L, Savastano M, Azzolina R, Grillo C, Messina A, Serra A, Tsami A: Oxidative stress, nitric oxide, endothelial dysfunction and tinnitus. Free Radic Res. 2006 Jun;40(6):615-8. [PubMed:16753839 ]
Atiya A, Cohen G, Ignarro L, Brunicardi FC: Nitric oxide regulates insulin secretion in the isolated perfused human pancreas via a cholinergic mechanism. Surgery. 1996 Aug;120(2):322-7. [PubMed:8751600 ]
Kim N, Vardi Y, Padma-Nathan H, Daley J, Goldstein I, Saenz de Tejada I: Oxygen tension regulates the nitric oxide pathway. Physiological role in penile erection. J Clin Invest. 1993 Feb;91(2):437-42. [PubMed:7679408 ]
Nichols K, Staines W, Krantis A: Neural sites of the human colon colocalize nitric oxide synthase-related NADPH diaphorase activity and neuropeptide Y. Gastroenterology. 1994 Oct;107(4):968-75. [PubMed:7523222 ]
Reitz A, Knapp PA, Muntener M, Schurch B: Oral nitric oxide donors: a new pharmacological approach to detrusor-sphincter dyssynergia in spinal cord injured patients? Eur Urol. 2004 Apr;45(4):516-20. [PubMed:15041118 ]
Huguenin S, Vacherot F, Fleury-Feith J, Riffaud JP, Chopin DK, Bolla M, Jaurand MC: Evaluation of the antitumoral potential of different nitric oxide-donating non-steroidal anti-inflammatory drugs (NO-NSAIDs) on human urological tumor cell lines. Cancer Lett. 2005 Feb 10;218(2):163-70. [PubMed:15670893 ]
Stack WA, Filipowicz B, Hawkey CJ: Nitric oxide donating compounds stimulate human colonic ion transport in vitro. Gut. 1996 Jul;39(1):93-9. [PubMed:8881817 ]
Khanna A, Cowled PA, Fitridge RA: Nitric oxide and skeletal muscle reperfusion injury: current controversies (research review). J Surg Res. 2005 Sep;128(1):98-107. [PubMed:15961106 ]
Calka J: The role of nitric oxide in the hypothalamic control of LHRH and oxytocin release, sexual behavior and aging of the LHRH and oxytocin neurons. Folia Histochem Cytobiol. 2006;44(1):3-12. [PubMed:16584085 ]
Kublickiene KR, Cockell AP, Nisell H, Poston L: Role of nitric oxide in the regulation of vascular tone in pressurized and perfused resistance myometrial arteries from term pregnant women. Am J Obstet Gynecol. 1997 Nov;177(5):1263-9. [PubMed:9396927 ]
Ormerod AD, Copeland P, Hay I, Husain A, Ewen SW: The inflammatory and cytotoxic effects of a nitric oxide releasing cream on normal skin. J Invest Dermatol. 1999 Sep;113(3):392-7. [PubMed:10469339 ]
Slaghekke F, Dekker G, Jeffries B: Endogenous inhibitors of nitric oxide and preeclampsia: a review. J Matern Fetal Neonatal Med. 2006 Aug;19(8):447-52. [PubMed:16966108 ]
Hurford WE: Current status of nitric oxide inhalation. Nihon Kyobu Shikkan Gakkai Zasshi. 1995 Dec;33 Suppl:199-204. [PubMed:8752507 ]
Stephens C, Fawcett TN: Nitric oxide and nursing: a review. J Clin Nurs. 2007 Jan;16(1):67-76. [PubMed:17181668 ]
Bernstein HG, Bogerts B, Keilhoff G: The many faces of nitric oxide in schizophrenia. A review. Schizophr Res. 2005 Oct 1;78(1):69-86. [PubMed:16005189 ]
(). EAFUS: Everything Added to Food in the United States.. .

Enzymes

Enzyme Details

1. Nitric oxide synthase, inducible

General function:: Involved in oxidoreductase activity
Specific function:: Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2.
Gene Name:: NOS2
Uniprot ID:: P35228
Molecular weight:: 131116.3

Reactions

L-Arginine + NADPH + Oxygen → Citrulline + Nitric oxide + NADP + Water	details
NADPH + N(omega)-Hydroxyarginine + Oxygen + Hydrogen Ion → NADP + Nitric oxide + Citrulline + Water	details
L-Arginine + Oxygen + NADPH + Hydrogen Ion → Nitric oxide + Citrulline + NADP + Water	details

Enzyme Details

2. Nitric oxide synthase, brain

General function:: Involved in oxidoreductase activity
Specific function:: Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter. Probably has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such SRR.
Gene Name:: NOS1
Uniprot ID:: P29475
Molecular weight:: 160969.095

Reactions

L-Arginine + NADPH + Oxygen → Citrulline + Nitric oxide + NADP + Water	details
NADPH + N(omega)-Hydroxyarginine + Oxygen + Hydrogen Ion → NADP + Nitric oxide + Citrulline + Water	details
L-Arginine + Oxygen + NADPH + Hydrogen Ion → Nitric oxide + Citrulline + NADP + Water	details

Enzyme Details

3. Nitric oxide synthase, endothelial

General function:: Involved in oxidoreductase activity
Specific function:: Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets. Isoform eNOS13C: Lacks eNOS activity, dominant-negative form that may down-regulate eNOS activity by forming heterodimers with isoform 1.
Gene Name:: NOS3
Uniprot ID:: P29474
Molecular weight:: 133273.59

Reactions

L-Arginine + NADPH + Oxygen → Citrulline + Nitric oxide + NADP + Water	details
NADPH + N(omega)-Hydroxyarginine + Oxygen + Hydrogen Ion → NADP + Nitric oxide + Citrulline + Water	details
L-Arginine + Oxygen + NADPH + Hydrogen Ion → Nitric oxide + Citrulline + NADP + Water	details

Enzyme Details

4. Hemoglobin subunit beta

General function:: Involved in iron ion binding
Specific function:: LVV-hemorphin-7 potentiates the activity of bradykinin, causing a decrease in blood pressure
Gene Name:: HBB
Uniprot ID:: P68871
Molecular weight:: 15998.3

Enzyme Details

5. Endothelin-1

General function:: Involved in endothelin A receptor binding
Specific function:: Endothelins are endothelium-derived vasoconstrictor peptides
Gene Name:: EDN1
Uniprot ID:: P05305
Molecular weight:: 24424.9

Enzyme Details

6. NOS1 mutant

General function:: Involved in oxidoreductase activity
Specific function:: Not Available
Gene Name:: NOS1
Uniprot ID:: B3VK56
Molecular weight:: 160969.1

Enzyme Details

7. Dynein light chain 1, cytoplasmic

General function:: Involved in microtubule-based process
Specific function:: Regulates apoptotic activities of BCL2L11 by sequestering it to microtubules. Upon apoptotic stimuli the BCL2L11-DYNLL1 complex dissociates from cytoplasmic dynein and translocates to mitochondria and sequesters BCL2 thus neutralizing its antiapoptotic activity
Gene Name:: DYNLL1
Uniprot ID:: P63167
Molecular weight:: 10365.8

Showing metabocard for Nitric oxide (HMDB0003378)

MOL for HMDB0003378 (Nitric oxide)

3D MOL for HMDB0003378 (Nitric oxide)

3D SDF for HMDB0003378 (Nitric oxide)

3D-SDF for HMDB0003378 (Nitric oxide)

PDB for HMDB0003378 (Nitric oxide)

3D PDB for HMDB0003378 (Nitric oxide)

SMILES for HMDB0003378 (Nitric oxide)

INCHI for HMDB0003378 (Nitric oxide)

Structure for HMDB0003378 (Nitric oxide)

3D Structure for HMDB0003378 (Nitric oxide)

Predicted Collision Cross Sections

Predicted Kovats Retention Indices

Underivatized

GC-MS Spectra

MS/MS Spectra

Enzymes

Reactions

Reactions

Reactions