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Positive bloodstream nucleic acid pathogen test

MedGen UID:
1053506
•Concept ID:
CN376477
•
Finding
HPO: HP:0430067

Definition

Positive (abnormal) result of a test such as polymerase chain reaction that is targeted against a nucleic acid sequence specific for a pathogen. [from HPO]

Professional guidelines

PubMed

Antibiotic treatment algorithm development based on a microarray nucleic acid assay for rapid bacterial identification and resistance determination from positive blood cultures.
Rödel J, Karrasch M, Edel B, Stoll S, Bohnert J, Löffler B, Saupe A, Pfister W
Diagn Microbiol Infect Dis 2016 Mar;84(3):252-7. Epub 2015 Nov 14 doi: 10.1016/j.diagmicrobio.2015.10.021. PMID: 26712265

Recent clinical studies

Etiology

Comprehensive evaluation of plasma microbial cell-free DNA sequencing for predicting bloodstream and local infections in clinical practice: a multicenter retrospective study.
Pang F, Xu W, Zhao H, Chen S, Tian Y, Fu J, You Z, Song P, Xian Q, Zhao Q, Wang C, Jia X
Front Cell Infect Microbiol 2023;13:1256099. Epub 2024 Jan 4 doi: 10.3389/fcimb.2023.1256099. PMID: 38362158Free PMC Article
Performance of PCR-REBA assay for screening and identifying pathogens directly in whole blood of patients with suspected sepsis.
Wang HY, Kim J, Kim S, Park SD, Kim HY, Choi HK, Uh Y, Lee H
J Appl Microbiol 2015 Nov;119(5):1433-42. Epub 2015 Oct 7 doi: 10.1111/jam.12941. PMID: 26299262
Association of Altered Serum MicroRNAs with Perihematomal Edema after Acute Intracerebral Hemorrhage.
Zhu Y, Wang JL, He ZY, Jin F, Tang L
PLoS One 2015;10(7):e0133783. Epub 2015 Jul 24 doi: 10.1371/journal.pone.0133783. PMID: 26207814Free PMC Article
SeptiFast for diagnosis of sepsis in severely ill patients from a Brazilian hospital.
Sitnik R, Marra AR, Petroni RC, Ramos OP, Martino MD, Pasternak J, Santos OF, Mangueira CL, Pinho JR
Einstein (Sao Paulo) 2014 Apr;12(2):191-6. doi: 10.1590/s1679-45082014ao2932. PMID: 25003925Free PMC Article
Anti-NR2 glutamate receptor antibodies and cognitive function in systemic lupus erythematosus.
Hanly JG, Robichaud J, Fisk JD
J Rheumatol 2006 Aug;33(8):1553-8. PMID: 16881112

Diagnosis

Clinical evaluation of cell-direct polymerase chain reaction-based nucleic acid lateral flow immunoassay for rapid detection of bacterial pathogens in clinically suspected sepsis: A multi-center study in Japan.
Shibata W, Uchida K, Ozawa D, Imai H, Endo S, Watanabe Y, Kosai K, Kawamoto Y, Uehara H, Sugimoto N, Namikawa H, Mizobata Y, Keiji K, Yano H, Kaku M, Seki M, Yanagihara K, Kakeya H
J Microbiol Methods 2022 Oct;201:106566. Epub 2022 Sep 8 doi: 10.1016/j.mimet.2022.106566. PMID: 36087825
A Closer Look at the Laboratory Impact of Utilizing ePlex Blood Culture Identification Panels: a Workflow Analysis Using Rapid Molecular Detection for Positive Blood Cultures.
Tansarli GS, Chapin KC
Microbiol Spectr 2022 Oct 26;10(5):e0179622. Epub 2022 Sep 7 doi: 10.1128/spectrum.01796-22. PMID: 36069598Free PMC Article
Direct Detection of Pathogens in Bloodstream During Sepsis: Are We There Yet?
Samuel L
J Appl Lab Med 2019 Jan;3(4):631-642. Epub 2018 Nov 30 doi: 10.1373/jalm.2018.028274. PMID: 31639731
Identification of bacterial and fungal pathogens from positive blood culture bottles: a microarray-based approach.
Raich T, Powell S
Methods Mol Biol 2015;1237:73-90. doi: 10.1007/978-1-4939-1776-1_8. PMID: 25319781
Verigene® gram-positive blood culture nucleic acid test.
Scott LJ
Mol Diagn Ther 2013 Apr;17(2):117-22. doi: 10.1007/s40291-013-0021-z. PMID: 23345071

Therapy

Bloodstream infection by Rhodococcus corynebacterioides in a pediatric patient diagnosed with high-risk retinoblastoma.
Méndez-Cruz AR, Félix-Bermúdez GE, Aguilar-Escobar DV, Vega-Vega L, Morales-Estrada AI, Contreras-Rodríguez A
Rev Argent Microbiol 2023 Jan-Mar;55(1):68-72. Epub 2022 Aug 18 doi: 10.1016/j.ram.2022.06.001. PMID: 35988003
First human case of catheter-related blood stream infection caused by Staphylococcus schleiferi subspecies coagulans: a case report and literature review.
Kobayashi T, Ikeda M, Ohama Y, Murono K, Ikeuchi K, Kitaura S, Okamoto K, Okugawa S, Ishihara S, Moriya K
Ann Clin Microbiol Antimicrob 2021 Sep 25;20(1):68. doi: 10.1186/s12941-021-00474-3. PMID: 34563226Free PMC Article
Direct Detection of Pathogens in Bloodstream During Sepsis: Are We There Yet?
Samuel L
J Appl Lab Med 2019 Jan;3(4):631-642. Epub 2018 Nov 30 doi: 10.1373/jalm.2018.028274. PMID: 31639731
Antibiotic treatment algorithm development based on a microarray nucleic acid assay for rapid bacterial identification and resistance determination from positive blood cultures.
Rödel J, Karrasch M, Edel B, Stoll S, Bohnert J, Löffler B, Saupe A, Pfister W
Diagn Microbiol Infect Dis 2016 Mar;84(3):252-7. Epub 2015 Nov 14 doi: 10.1016/j.diagmicrobio.2015.10.021. PMID: 26712265
A multicenter trial to compare blood culture with polymerase chain reaction in severe human sepsis.
Bloos F, Hinder F, Becker K, Sachse S, Mekontso Dessap A, Straube E, Cattoir V, Brun-Buisson C, Reinhart K, Peters G, Bauer M
Intensive Care Med 2010 Feb;36(2):241-7. Epub 2009 Nov 19 doi: 10.1007/s00134-009-1705-z. PMID: 19924398

Prognosis

Comprehensive evaluation of plasma microbial cell-free DNA sequencing for predicting bloodstream and local infections in clinical practice: a multicenter retrospective study.
Pang F, Xu W, Zhao H, Chen S, Tian Y, Fu J, You Z, Song P, Xian Q, Zhao Q, Wang C, Jia X
Front Cell Infect Microbiol 2023;13:1256099. Epub 2024 Jan 4 doi: 10.3389/fcimb.2023.1256099. PMID: 38362158Free PMC Article
Evaluation of a Commercial Multiplex PCR Assay for Detection of Pathogen DNA in Blood from Patients with Suspected Sepsis.
Ziegler I, Fagerström A, Strålin K, Mölling P
PLoS One 2016;11(12):e0167883. Epub 2016 Dec 20 doi: 10.1371/journal.pone.0167883. PMID: 27997618Free PMC Article
Performance of PCR-REBA assay for screening and identifying pathogens directly in whole blood of patients with suspected sepsis.
Wang HY, Kim J, Kim S, Park SD, Kim HY, Choi HK, Uh Y, Lee H
J Appl Microbiol 2015 Nov;119(5):1433-42. Epub 2015 Oct 7 doi: 10.1111/jam.12941. PMID: 26299262
Identification of the most common pathogenic bacteria in patients with suspected sepsis by multiplex PCR.
Arabestani MR, Fazzeli H, Nasr Esfahani B
J Infect Dev Ctries 2014 Apr 15;8(4):461-8. doi: 10.3855/jidc.3856. PMID: 24727512
Verigene® gram-positive blood culture nucleic acid test.
Scott LJ
Mol Diagn Ther 2013 Apr;17(2):117-22. doi: 10.1007/s40291-013-0021-z. PMID: 23345071

Clinical prediction guides

Comprehensive evaluation of plasma microbial cell-free DNA sequencing for predicting bloodstream and local infections in clinical practice: a multicenter retrospective study.
Pang F, Xu W, Zhao H, Chen S, Tian Y, Fu J, You Z, Song P, Xian Q, Zhao Q, Wang C, Jia X
Front Cell Infect Microbiol 2023;13:1256099. Epub 2024 Jan 4 doi: 10.3389/fcimb.2023.1256099. PMID: 38362158Free PMC Article
Bloodstream and catheter-related infections due to different clones of multidrug-resistant and biofilm producer Corynebacterium striatum.
Ramos JN, Souza C, Faria YV, da Silva EC, Veras JFC, Baio PVP, Seabra SH, de Oliveira Moreira L, Hirata Júnior R, Mattos-Guaraldi AL, Vieira VV
BMC Infect Dis 2019 Jul 29;19(1):672. doi: 10.1186/s12879-019-4294-7. PMID: 31357945Free PMC Article
Identification of the most common pathogenic bacteria in patients with suspected sepsis by multiplex PCR.
Arabestani MR, Fazzeli H, Nasr Esfahani B
J Infect Dev Ctries 2014 Apr 15;8(4):461-8. doi: 10.3855/jidc.3856. PMID: 24727512
Verigene® gram-positive blood culture nucleic acid test.
Scott LJ
Mol Diagn Ther 2013 Apr;17(2):117-22. doi: 10.1007/s40291-013-0021-z. PMID: 23345071
A multicenter trial to compare blood culture with polymerase chain reaction in severe human sepsis.
Bloos F, Hinder F, Becker K, Sachse S, Mekontso Dessap A, Straube E, Cattoir V, Brun-Buisson C, Reinhart K, Peters G, Bauer M
Intensive Care Med 2010 Feb;36(2):241-7. Epub 2009 Nov 19 doi: 10.1007/s00134-009-1705-z. PMID: 19924398

Recent systematic reviews

Multiplex PCR system for rapid detection of pathogens in patients with presumed sepsis - a systemic review and meta-analysis.
Chang SS, Hsieh WH, Liu TS, Lee SH, Wang CH, Chou HC, Yeo YH, Tseng CP, Lee CC
PLoS One 2013;8(5):e62323. Epub 2013 May 29 doi: 10.1371/journal.pone.0062323. PMID: 23734173Free PMC Article

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