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Chek2 checkpoint kinase 2 [ Rattus norvegicus (Norway rat) ]

Gene ID: 114212, updated on 11-Apr-2024

Summary

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Official Symbol
Chek2provided by RGD
Official Full Name
checkpoint kinase 2provided by RGD
Primary source
RGD:621543
See related
Ensembl:ENSRNOG00000037509 AllianceGenome:RGD:621543
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Rattus norvegicus
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as
Chk2; Rad53
Summary
Enables protein serine/threonine kinase activity. Involved in several processes, including cellular response to bisphenol A; positive regulation of anoikis; and regulation of cell cycle phase transition. Located in nucleus. Biomarker of urinary bladder cancer. Human ortholog(s) of this gene implicated in several diseases, including Li-Fraumeni syndrome 2; breast cancer (multiple); colorectal cancer; osteosarcoma; and prostate cancer. Orthologous to human CHEK2 (checkpoint kinase 2). [provided by Alliance of Genome Resources, Apr 2022]
Expression
Biased expression in Thymus (RPKM 60.6), Spleen (RPKM 55.8) and 9 other tissues See more
Orthologs
human mouse all
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Genomic context

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Location:
12q16
Exon count:
16
Annotation release Status Assembly Chr Location
RS_2024_02 current GRCr8 (GCF_036323735.1) 12 NC_086030.1 (51448838..51481159, complement)
RS_2023_06 previous assembly mRatBN7.2 (GCF_015227675.2) 12 NC_051347.1 (45788823..45821382, complement)
106 previous assembly Rnor_6.0 (GCF_000001895.5) 12 NC_005111.4 (51845574..51878098, complement)

Chromosome 12 - NC_086030.1Genomic Context describing neighboring genes Neighboring gene tetratricopeptide repeat domain 28 Neighboring gene uncharacterized LOC102553561 Neighboring gene uncharacterized LOC120096015 Neighboring gene HscB mitochondrial iron-sulfur cluster co-chaperone Neighboring gene unc-51 like autophagy activating kinase 1

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
  • Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
  • BioProject: PRJNA238328
  • Publication: PMID 24510058
  • Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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Related articles in PubMed

  1. Bisphenol A induces oxidative stress-associated DNA damage in INS-1 cells. Xin F, et al. Mutat Res Genet Toxicol Environ Mutagen, 2014 Jul 15. PMID 25344109
  2. Premature cardiac senescence in DahlS.Z-Lepr(fa)/Lepr(fa) rats as a new animal model of metabolic syndrome. Takahashi K, et al. Nagoya J Med Sci, 2014 Feb. PMID 25129990, Free PMC Article
  3. Cellular distribution of cell cycle-related molecules in the renal tubules of rats treated with renal carcinogens for 28 days: relationship between cell cycle aberration and carcinogenesis. Taniai E, et al. Arch Toxicol, 2012 Sep. PMID 22411272
  4. Tumor suppressor protein kinase Chk2 is a mediator of anoikis of intestinal epithelial cells. Yoo BH, et al. Int J Cancer, 2012 Jul 15. PMID 21834075
  5. Paeonia lactiflora Pall inhibits bladder cancer growth involving phosphorylation of Chk2 in vitro and in vivo. Ou TT, et al. J Ethnopharmacol, 2011 Apr 26. PMID 21396995

See all (46) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Chk-2 is an important novel component of anoikis-promoting machinery of intestinal epithelial cells
    Title: Tumor suppressor protein kinase Chk2 is a mediator of anoikis of intestinal epithelial cells.
  2. Data demonstrate that polyamines regulate c-Myc translation in IECs through HuR phosphorylation by Chk2 and provide new insight into the molecular functions of cellular polyamines.
    Title: Polyamines regulate c-Myc translation through Chk2-dependent HuR phosphorylation.
  3. an important role for the DNA damage response mediated by ATR-Chk2 in p53 activation and renal cell apoptosis during cisplatin nephrotoxicity.
    Title: ATR-Chk2 signaling in p53 activation and DNA damage response during cisplatin-induced apoptosis.
Submit: New GeneRIF Correction

Pathways from PubChem

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Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Markers

Homology

Gene Ontology Provided by RGD

Function Evidence Code Pubs
enables ATP binding IEA
Inferred from Electronic Annotation
more info
  
enables identical protein binding ISO
Inferred from Sequence Orthology
more info
  
enables kinase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables protein homodimerization activity ISO
Inferred from Sequence Orthology
more info
  
enables protein kinase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables protein kinase activity ISO
Inferred from Sequence Orthology
more info
  
enables protein kinase binding ISO
Inferred from Sequence Orthology
more info
  
enables protein serine/threonine kinase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables protein serine/threonine kinase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables protein serine/threonine kinase activity ISO
Inferred from Sequence Orthology
more info
  
enables ubiquitin protein ligase binding ISO
Inferred from Sequence Orthology
more info
  
Process Evidence Code Pubs
involved_in DNA damage checkpoint signaling IMP
Inferred from Mutant Phenotype
more info
 PubMed 
acts_upstream_of_or_within DNA damage response ISO
Inferred from Sequence Orthology
more info
  
involved_in DNA damage response ISO
Inferred from Sequence Orthology
more info
  
acts_upstream_of_or_within DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator ISO
Inferred from Sequence Orthology
more info
  
involved_in G2/M transition of mitotic cell cycle ISO
Inferred from Sequence Orthology
more info
  
involved_in cellular response to bisphenol A IEP
Inferred from Expression Pattern
more info
 PubMed 
acts_upstream_of_or_within cellular response to gamma radiation ISO
Inferred from Sequence Orthology
more info
  
involved_in cellular response to xenobiotic stimulus IEP
Inferred from Expression Pattern
more info
 PubMed 
involved_in double-strand break repair ISO
Inferred from Sequence Orthology
more info
  
involved_in intrinsic apoptotic signaling pathway in response to DNA damage ISO
Inferred from Sequence Orthology
more info
  
acts_upstream_of_or_within intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator ISO
Inferred from Sequence Orthology
more info
  
involved_in mitotic DNA damage checkpoint signaling IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in mitotic intra-S DNA damage checkpoint signaling ISO
Inferred from Sequence Orthology
more info
  
involved_in mitotic spindle assembly ISO
Inferred from Sequence Orthology
more info
  
involved_in negative regulation of DNA damage checkpoint IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in positive regulation of DNA-templated transcription ISO
Inferred from Sequence Orthology
more info
  
involved_in positive regulation of anoikis IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in positive regulation of protein phosphorylation IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in positive regulation of protein phosphorylation IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in protein catabolic process ISO
Inferred from Sequence Orthology
more info
  
involved_in protein stabilization ISO
Inferred from Sequence Orthology
more info
  
involved_in regulation of DNA-templated transcription ISO
Inferred from Sequence Orthology
more info
  
involved_in regulation of protein catabolic process ISO
Inferred from Sequence Orthology
more info
  
acts_upstream_of_or_within response to gamma radiation ISO
Inferred from Sequence Orthology
more info
  
involved_in response to glycoside IEP
Inferred from Expression Pattern
more info
 PubMed 
acts_upstream_of_or_within signal transduction in response to DNA damage ISO
Inferred from Sequence Orthology
more info
  
involved_in signal transduction in response to DNA damage ISO
Inferred from Sequence Orthology
more info
  
acts_upstream_of_or_within thymocyte apoptotic process ISO
Inferred from Sequence Orthology
more info
  
Component Evidence Code Pubs
located_in Golgi apparatus ISO
Inferred from Sequence Orthology
more info
  
located_in PML body ISO
Inferred from Sequence Orthology
more info
  
located_in chromosome, telomeric region ISO
Inferred from Sequence Orthology
more info
  
is_active_in cytoplasm IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in nucleoplasm ISO
Inferred from Sequence Orthology
more info
  
is_active_in nucleus IBA
Inferred from Biological aspect of Ancestor
more info
  

General protein information

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Preferred Names
serine/threonine-protein kinase Chk2
Names
CHK2 checkpoint homolog
protein kinase Chk2
NP_446129.1
XP_006249580.1
XP_006249581.1
XP_006249582.1
XP_006249583.1
XP_063127056.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_053677.1 → NP_446129.1  serine/threonine-protein kinase Chk2

    See identical proteins and their annotated locations for NP_446129.1

    Status: PROVISIONAL

    Source sequence(s)
    AF134054
    UniProtKB/TrEMBL
    A0A0G2K8L3, A6J285, F7F4E2, Q9R019
    Conserved Domains (2) summary
    cd00060
    Location:96 → 204
    FHA; Forkhead associated domain (FHA); found in eukaryotic and prokaryotic proteins. Putative nuclear signalling domain. FHA domains may bind phosphothreonine, phosphoserine and sometimes phosphotyrosine. In eukaryotes, many FHA domain-containing proteins ...
    cd14084
    Location:216 → 489
    STKc_Chk2; Catalytic domain of the Serine/Threonine kinase, Cell cycle Checkpoint Kinase 2

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

  1. NC_086030.1 Reference GRCr8

    Range
    51448838..51481159 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_006249519.4 → XP_006249581.1  serine/threonine-protein kinase Chk2 isoform X1

    See identical proteins and their annotated locations for XP_006249581.1

    UniProtKB/TrEMBL
    A0A0G2K8L3, A6J285, F7F4E2, Q9R019
    Related
    ENSRNOP00000074651.1
    Conserved Domains (2) summary
    cd00060
    Location:96 → 204
    FHA; Forkhead associated domain (FHA); found in eukaryotic and prokaryotic proteins. Putative nuclear signalling domain. FHA domains may bind phosphothreonine, phosphoserine and sometimes phosphotyrosine. In eukaryotes, many FHA domain-containing proteins ...
    cd14084
    Location:216 → 489
    STKc_Chk2; Catalytic domain of the Serine/Threonine kinase, Cell cycle Checkpoint Kinase 2

  2. XM_006249521.5 → XP_006249583.1  serine/threonine-protein kinase Chk2 isoform X1

    See identical proteins and their annotated locations for XP_006249583.1

    UniProtKB/TrEMBL
    A0A0G2K8L3, A6J285, F7F4E2, Q9R019
    Conserved Domains (2) summary
    cd00060
    Location:96 → 204
    FHA; Forkhead associated domain (FHA); found in eukaryotic and prokaryotic proteins. Putative nuclear signalling domain. FHA domains may bind phosphothreonine, phosphoserine and sometimes phosphotyrosine. In eukaryotes, many FHA domain-containing proteins ...
    cd14084
    Location:216 → 489
    STKc_Chk2; Catalytic domain of the Serine/Threonine kinase, Cell cycle Checkpoint Kinase 2

  3. XM_006249518.5 → XP_006249580.1  serine/threonine-protein kinase Chk2 isoform X1

    See identical proteins and their annotated locations for XP_006249580.1

    UniProtKB/TrEMBL
    A0A0G2K8L3, A6J285, F7F4E2, Q9R019
    Conserved Domains (2) summary
    cd00060
    Location:96 → 204
    FHA; Forkhead associated domain (FHA); found in eukaryotic and prokaryotic proteins. Putative nuclear signalling domain. FHA domains may bind phosphothreonine, phosphoserine and sometimes phosphotyrosine. In eukaryotes, many FHA domain-containing proteins ...
    cd14084
    Location:216 → 489
    STKc_Chk2; Catalytic domain of the Serine/Threonine kinase, Cell cycle Checkpoint Kinase 2

  4. XM_006249520.5 → XP_006249582.1  serine/threonine-protein kinase Chk2 isoform X1

    See identical proteins and their annotated locations for XP_006249582.1

    UniProtKB/TrEMBL
    A0A0G2K8L3, A6J285, F7F4E2, Q9R019
    Conserved Domains (2) summary
    cd00060
    Location:96 → 204
    FHA; Forkhead associated domain (FHA); found in eukaryotic and prokaryotic proteins. Putative nuclear signalling domain. FHA domains may bind phosphothreonine, phosphoserine and sometimes phosphotyrosine. In eukaryotes, many FHA domain-containing proteins ...
    cd14084
    Location:216 → 489
    STKc_Chk2; Catalytic domain of the Serine/Threonine kinase, Cell cycle Checkpoint Kinase 2

  5. XM_063270986.1 → XP_063127056.1  serine/threonine-protein kinase Chk2 isoform X2

Nucleotide Protein Strain
Heading Accession and Version

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