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Cyb5r4 cytochrome b5 reductase 4 [ Rattus norvegicus (Norway rat) ]

Gene ID: 171015, updated on 11-Apr-2024

Summary

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Official Symbol
Cyb5r4provided by RGD
Official Full Name
cytochrome b5 reductase 4provided by RGD
Primary source
RGD:621834
See related
Ensembl:ENSRNOG00000010024 AllianceGenome:RGD:621834
Gene type
protein coding
RefSeq status
VALIDATED
Organism
Rattus norvegicus
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as
Ncb5or; b5&b5R; b5+b5R; cb5/cb5R
Summary
Enables NADPH-hemoprotein reductase activity; flavin adenine dinucleotide binding activity; and heme binding activity. Involved in NADP metabolic process. Predicted to be located in perinuclear region of cytoplasm. Predicted to be active in endoplasmic reticulum. Human ortholog(s) of this gene implicated in type 2 diabetes mellitus. Orthologous to human CYB5R4 (cytochrome b5 reductase 4). [provided by Alliance of Genome Resources, Apr 2022]
Expression
Biased expression in Kidney (RPKM 180.1), Thymus (RPKM 149.1) and 9 other tissues See more
Orthologs
human all
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Genomic context

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See Cyb5r4 in Genome Data Viewer
Location:
8q31
Exon count:
16
Annotation release Status Assembly Chr Location
RS_2024_02 current GRCr8 (GCF_036323735.1) 8 NC_086026.1 (96860415..96925789)
RS_2023_06 previous assembly mRatBN7.2 (GCF_015227675.2) 8 NC_051343.1 (87975596..88048356)
106 previous assembly Rnor_6.0 (GCF_000001895.5) 8 NC_005107.4 (94693273..94757989)

Chromosome 8 - NC_086026.1Genomic Context describing neighboring genes Neighboring gene synaptosome associated protein 91 Neighboring gene ripply transcriptional repressor 2 Neighboring gene melanocortin 2 receptor accessory protein 2 Neighboring gene centrosomal protein 162

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
  • Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
  • BioProject: PRJNA238328
  • Publication: PMID 24510058
  • Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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Related articles in PubMed

  1. Mutagenesis of Glycine 179 modulates both catalytic efficiency and reduced pyridine nucleotide specificity in cytochrome b5 reductase. Roma GW, et al. Biochemistry, 2005 Oct 18. PMID 16216070
  2. The reductase NCB5OR is responsive to the redox status in beta-cells and is not involved in the ER stress response. Larade K, et al. Biochem J, 2007 Jun 15. PMID 17343567, Free PMC Article
  3. Variation in NCB5OR: studies of relationships to type 2 diabetes, maturity-onset diabetes of the young, and gestational diabetes mellitus. Andersen G, et al. Diabetes, 2004 Nov. PMID 15504981, Free PMC Article
  4. Absence of a reductase, NCB5OR, causes insulin-deficient diabetes. Xie J, et al. Proc Natl Acad Sci U S A, 2004 Jul 20. PMID 15247412, Free PMC Article
  5. Identification and characterization of a novel splice variant of mouse and rat cytochrome b5/cytochrome b5 reductase. Curry BJ, et al. Genomics, 2004 Mar. PMID 14962668

See all (10) citations in PubMed

GeneRIFs: Gene References Into Functions

General gene information

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Markers

Homology

Gene Ontology Provided by RGD

Function Evidence Code Pubs
enables NAD(P)H oxidase H2O2-forming activity IEA
Inferred from Electronic Annotation
more info
  
NOT enables NAD(P)H oxidase H2O2-forming activity ISO
Inferred from Sequence Orthology
more info
  
enables NAD(P)H oxidase H2O2-forming activity ISO
Inferred from Sequence Orthology
more info
  
enables NADPH-hemoprotein reductase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables cytochrome-b5 reductase activity, acting on NAD(P)H IBA
Inferred from Biological aspect of Ancestor
more info
  
enables cytochrome-b5 reductase activity, acting on NAD(P)H IEA
Inferred from Electronic Annotation
more info
  
enables cytochrome-b5 reductase activity, acting on NAD(P)H ISO
Inferred from Sequence Orthology
more info
  
enables flavin adenine dinucleotide binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables heme binding IBA
Inferred from Biological aspect of Ancestor
more info
  
enables heme binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables heme binding IEA
Inferred from Electronic Annotation
more info
  
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
enables oxidoreductase activity, acting on NAD(P)H, heme protein as acceptor IEA
Inferred from Electronic Annotation
more info
  
enables oxidoreductase activity, acting on NAD(P)H, heme protein as acceptor ISO
Inferred from Sequence Orthology
more info
  
Process Evidence Code Pubs
involved_in NADP metabolic process IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in cell development IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within cell development ISO
Inferred from Sequence Orthology
more info
  
involved_in glucose homeostasis IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within glucose homeostasis ISO
Inferred from Sequence Orthology
more info
  
involved_in insulin secretion IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within insulin secretion ISO
Inferred from Sequence Orthology
more info
  
involved_in reactive oxygen species metabolic process ISO
Inferred from Sequence Orthology
more info
  
involved_in response to antibiotic IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within response to antibiotic ISO
Inferred from Sequence Orthology
more info
  
involved_in superoxide metabolic process IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in superoxide metabolic process IEA
Inferred from Electronic Annotation
more info
  
involved_in superoxide metabolic process ISO
Inferred from Sequence Orthology
more info
  
Component Evidence Code Pubs
is_active_in cytoplasm IBA
Inferred from Biological aspect of Ancestor
more info
  
is_active_in endoplasmic reticulum IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in endoplasmic reticulum IEA
Inferred from Electronic Annotation
more info
  
located_in endoplasmic reticulum ISO
Inferred from Sequence Orthology
more info
  
located_in perinuclear region of cytoplasm IEA
Inferred from Electronic Annotation
more info
  
located_in perinuclear region of cytoplasm ISO
Inferred from Sequence Orthology
more info
  

General protein information

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Preferred Names
cytochrome b5 reductase 4
Names
N-terminal cytochrome b5 and cytochrome b5 oxidoreductase domain-containing protein
NADPH cytochrome B5 oxidoreductase
flavohemoprotein b5+b5R
flavohemoprotein b5/b5R
NP_596918.3
XP_006243534.1
XP_017450917.1
XP_038936668.1
XP_063120880.1
XP_063120881.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_133427.3 → NP_596918.3  cytochrome b5 reductase 4

    See identical proteins and their annotated locations for NP_596918.3

    Status: VALIDATED

    Source sequence(s)
    BC080240, JAXUCZ010000008
    UniProtKB/Swiss-Prot
    Q68EJ0, Q6VXY2, Q6VXY3, Q9EPZ4
    UniProtKB/TrEMBL
    A0A0G2JSK2, A6I1W7
    Related
    ENSRNOP00000013909.4, ENSRNOT00000013908.6
    Conserved Domains (3) summary
    cd06183
    Location:277 → 518
    cyt_b5_reduct_like; Cytochrome b5 reductase catalyzes the reduction of 2 molecules of cytochrome b5 using NADH as an electron donor. Like ferredoxin reductases, these proteins have an N-terminal FAD binding subdomain and a C-terminal NADH binding subdomain, separated by a ...
    cd06490
    Location:169 → 255
    p23_NCB5OR; p23_like domain found in NAD(P)H cytochrome b5 (NCB5) oxidoreductase (OR) and similar proteins. NCB5OR is widely expressed in human organs and tissues and is localized in the ER (endoplasmic reticulum). It appears to play a critical role in maintaining ...
    pfam00173
    Location:56 → 128
    Cyt-b5; Cytochrome b5-like Heme/Steroid binding domain

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

  1. NC_086026.1 Reference GRCr8

    Range
    96860415..96925789
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_039080740.2 → XP_038936668.1  cytochrome b5 reductase 4 isoform X2

    UniProtKB/TrEMBL
    A6I1W6
    Conserved Domains (3) summary
    cd06183
    Location:243 → 484
    cyt_b5_reduct_like; Cytochrome b5 reductase catalyzes the reduction of 2 molecules of cytochrome b5 using NADH as an electron donor. Like ferredoxin reductases, these proteins have an N-terminal FAD binding subdomain and a C-terminal NADH binding subdomain, separated by a ...
    cd06490
    Location:135 → 221
    p23_NCB5OR; p23_like domain found in NAD(P)H cytochrome b5 (NCB5) oxidoreductase (OR) and similar proteins. NCB5OR is widely expressed in human organs and tissues and is localized in the ER (endoplasmic reticulum). It appears to play a critical role in maintaining ...
    pfam00173
    Location:24 → 96
    Cyt-b5; Cytochrome b5-like Heme/Steroid binding domain

  2. XM_063264810.1 → XP_063120880.1  cytochrome b5 reductase 4 isoform X4

  3. XM_017595428.3 → XP_017450917.1  cytochrome b5 reductase 4 isoform X3

    UniProtKB/Swiss-Prot
    Q68EJ0, Q6VXY2, Q6VXY3, Q9EPZ4
    UniProtKB/TrEMBL
    A0A8I6GKQ4
    Related
    ENSRNOP00000093571.1

  4. XM_006243472.5 → XP_006243534.1  cytochrome b5 reductase 4 isoform X1

    UniProtKB/Swiss-Prot
    Q68EJ0, Q6VXY2, Q6VXY3, Q9EPZ4
    UniProtKB/TrEMBL
    A0A8I6A4B1
    Related
    ENSRNOP00000085891.1
    Conserved Domains (3) summary
    cd06183
    Location:267 → 508
    cyt_b5_reduct_like; Cytochrome b5 reductase catalyzes the reduction of 2 molecules of cytochrome b5 using NADH as an electron donor. Like ferredoxin reductases, these proteins have an N-terminal FAD binding subdomain and a C-terminal NADH binding subdomain, separated by a ...
    cd06490
    Location:159 → 245
    p23_NCB5OR; p23_like domain found in NAD(P)H cytochrome b5 (NCB5) oxidoreductase (OR) and similar proteins. NCB5OR is widely expressed in human organs and tissues and is localized in the ER (endoplasmic reticulum). It appears to play a critical role in maintaining ...
    pfam00173
    Location:56 → 128
    Cyt-b5; Cytochrome b5-like Heme/Steroid binding domain

  5. XM_063264811.1 → XP_063120881.1  cytochrome b5 reductase 4 isoform X5

Nucleotide Protein Strain
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q68EJ0.2 GenPept UniProtKB/Swiss-Prot:Q68EJ0

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