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Mgmt O-6-methylguanine-DNA methyltransferase [ Rattus norvegicus (Norway rat) ]

Gene ID: 25332, updated on 11-Apr-2024

Summary

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Official Symbol
Mgmtprovided by RGD
Official Full Name
O-6-methylguanine-DNA methyltransferaseprovided by RGD
Primary source
RGD:3087
See related
Ensembl:ENSRNOG00000016038 AllianceGenome:RGD:3087
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Rattus norvegicus
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Summary
Enables calcium ion binding activity and methylated-DNA-[protein]-cysteine S-methyltransferase activity. Involved in several processes, including DNA dealkylation involved in DNA repair; cellular response to ionizing radiation; and response to folic acid. Located in nucleus. Biomarker of colon adenocarcinoma and melanoma. Human ortholog(s) of this gene implicated in several diseases, including carcinoma (multiple); endocrine gland cancer (multiple); granulosa cell tumor; nervous system cancer (multiple); and urinary bladder cancer. Orthologous to human MGMT (O-6-methylguanine-DNA methyltransferase). [provided by Alliance of Genome Resources, Apr 2022]
Expression
Biased expression in Liver (RPKM 160.5), Spleen (RPKM 119.4) and 9 other tissues See more
Orthologs
human mouse all
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Genomic context

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See Mgmt in Genome Data Viewer
Location:
1q41
Exon count:
6
Annotation release Status Assembly Chr Location
RS_2024_02 current GRCr8 (GCF_036323735.1) 1 NC_086019.1 (201140832..201367481)
RS_2023_06 previous assembly mRatBN7.2 (GCF_015227675.2) 1 NC_051336.1 (191710980..191937760)
106 previous assembly Rnor_6.0 (GCF_000001895.5) 1 NC_005100.4 (209237255..209464189)

Chromosome 1 - NC_086019.1Genomic Context describing neighboring genes Neighboring gene uncharacterized LOC134485188 Neighboring gene cilia- and flagella-associated protein 251-like Neighboring gene uncharacterized LOC102552565 Neighboring gene EBF transcription factor 3

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
  • Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
  • BioProject: PRJNA238328
  • Publication: PMID 24510058
  • Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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Related articles in PubMed

  1. MGMT-Mediated neuron Apoptosis in Injured Rat Spinal Cord. Ni Y, et al. Tissue Cell, 2020 Feb. PMID 32433023
  2. Inhibition of poly(ADP-ribose) polymerase enhances the effect of chemotherapy in an animal model of regional therapy for the treatment of advanced extremity malignant melanoma. Toshimitsu H, et al. Ann Surg Oncol, 2010 Aug. PMID 20182810
  3. Discrimination of carcinogens by hepatic transcript profiling in rats following 28-day administration. Matsumoto H, et al. Cancer Inform, 2009 Nov 13. PMID 20011461, Free PMC Article
  4. O6-methylguanine DNA methyltransferase deficiency and response to temozolomide-based therapy in patients with neuroendocrine tumors. Kulke MH, et al. Clin Cancer Res, 2009 Jan 1. PMID 19118063, Free PMC Article
  5. Methylation profiles of multiple CpG island loci in extrahepatic cholangiocarcinoma versus those of intrahepatic cholangiocarcinomas. Kim BH, et al. Arch Pathol Lab Med, 2007 Jun. PMID 17550320

See all (48) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. MGMT-Mediated neuron Apoptosis in Injured Rat Spinal Cord.
    Title: MGMT-Mediated neuron Apoptosis in Injured Rat Spinal Cord.
  2. Folate deficiency resulted in significant increases in levels of the DNA repair proteins OGG-1 and MGMT in the liver
    Title: Folate deficiency alters hepatic and colon MGMT and OGG-1 DNA repair protein expression in rats but has no effect on genome-wide DNA methylation.
  3. age-related differences in MGMT activity may play a significant role in age-differential susceptibility to rat mammary gland and kidney carcinogenesis
    Title: Methylguanine methyltransferase activity deficiency in immature rat mammary epithelial cells parallels increased carcinogenic susceptibility.
  4. Divalent cations stabilized rat recombinant O6-methylguanine-DNA methyltransferase (rMGMT) protein against heat treatment
    Title: Induction of thermal and chemical stability of O6-methylguanine-DNA methyltransferase by Ca2+.
Submit: New GeneRIF Correction

General gene information

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Markers

Homology

Gene Ontology Provided by RGD

Function Evidence Code Pubs
enables DNA binding IEA
Inferred from Electronic Annotation
more info
  
enables calcium ion binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
enables methylated-DNA-[protein]-cysteine S-methyltransferase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables methylated-DNA-[protein]-cysteine S-methyltransferase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables methylated-DNA-[protein]-cysteine S-methyltransferase activity IEA
Inferred from Electronic Annotation
more info
  
enables methyltransferase activity ISO
Inferred from Sequence Orthology
more info
  
Process Evidence Code Pubs
involved_in DNA alkylation repair IDA
Inferred from Direct Assay
more info
 PubMed 
acts_upstream_of_or_within DNA alkylation repair ISO
Inferred from Sequence Orthology
more info
  
involved_in DNA damage response IEP
Inferred from Expression Pattern
more info
 PubMed 
acts_upstream_of_or_within DNA damage response ISO
Inferred from Sequence Orthology
more info
  
involved_in DNA modification IEA
Inferred from Electronic Annotation
more info
  
involved_in DNA repair IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in DNA repair IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within DNA repair ISO
Inferred from Sequence Orthology
more info
  
involved_in cellular response to ionizing radiation IEP
Inferred from Expression Pattern
more info
 PubMed 
involved_in cellular response to organic cyclic compound IEP
Inferred from Expression Pattern
more info
 PubMed 
involved_in cellular response to oxidative stress IEP
Inferred from Expression Pattern
more info
 PubMed 
involved_in mammary gland epithelial cell differentiation IEP
Inferred from Expression Pattern
more info
 PubMed 
involved_in methylation IEA
Inferred from Electronic Annotation
more info
  
involved_in negative regulation of apoptotic process IEA
Inferred from Electronic Annotation
more info
  
involved_in negative regulation of apoptotic process ISO
Inferred from Sequence Orthology
more info
  
involved_in positive regulation of DNA repair IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in positive regulation of double-strand break repair IEA
Inferred from Electronic Annotation
more info
  
involved_in positive regulation of double-strand break repair ISO
Inferred from Sequence Orthology
more info
  
involved_in response to ethanol IEP
Inferred from Expression Pattern
more info
 PubMed 
involved_in response to folic acid IEP
Inferred from Expression Pattern
more info
 PubMed 
involved_in response to organic cyclic compound IEP
Inferred from Expression Pattern
more info
 PubMed 
involved_in response to toxic substance IEP
Inferred from Expression Pattern
more info
 PubMed 
involved_in response to xenobiotic stimulus IEP
Inferred from Expression Pattern
more info
 PubMed 
Component Evidence Code Pubs
located_in membrane IEA
Inferred from Electronic Annotation
more info
  
located_in nucleoplasm IEA
Inferred from Electronic Annotation
more info
  
located_in nucleoplasm ISO
Inferred from Sequence Orthology
more info
  
is_active_in nucleus IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in nucleus IEA
Inferred from Electronic Annotation
more info
  

General protein information

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Preferred Names
methylated-DNA--protein-cysteine methyltransferase
Names
0-6-methylguanine-DNA methyltransferase
6-O-methylguanine-DNA methyltransferase
O(6)-alkylguanine-DNA alkyltransferase
O-6-methylguanine-DNA-alkyltransferase
O6-alkylguanine-DNA alkyltransferase
O6-methylguanine-DNA methyltranferase
NP_036993.1
XP_038957518.1
XP_063137786.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_012861.1 → NP_036993.1  methylated-DNA--protein-cysteine methyltransferase

    See identical proteins and their annotated locations for NP_036993.1

    Status: PROVISIONAL

    Source sequence(s)
    S61804
    UniProtKB/Swiss-Prot
    P24528
    UniProtKB/TrEMBL
    A0A8L2QAS3, A6HX75
    Related
    ENSRNOP00000079311.1, ENSRNOT00000119456.1
    Conserved Domains (3) summary
    COG0350
    Location:6 → 178
    AdaB; O6-methylguanine-DNA--protein-cysteine methyltransferase [Replication, recombination and repair]
    TIGR00589
    Location:97 → 177
    ogt; O-6-methylguanine DNA methyltransferase
    pfam02870
    Location:6 → 94
    Methyltransf_1N; 6-O-methylguanine DNA methyltransferase, ribonuclease-like domain

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

  1. NC_086019.1 Reference GRCr8

    Range
    201140832..201367481
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_063281716.1 → XP_063137786.1  methylated-DNA--protein-cysteine methyltransferase isoform X2

  2. XM_039101590.2 → XP_038957518.1  methylated-DNA--protein-cysteine methyltransferase isoform X1

    Conserved Domains (2) summary
    TIGR00589
    Location:58 → 138
    ogt; O-6-methylguanine DNA methyltransferase
    pfam02870
    Location:5 → 55
    Methyltransf_1N; 6-O-methylguanine DNA methyltransferase, ribonuclease-like domain
Nucleotide Protein Strain
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
P24528.3 GenPept UniProtKB/Swiss-Prot:P24528

Gene LinkOut

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