Mip major intrinsic protein of lens fiber [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Mipprovided by RGD
Official Full Name: major intrinsic protein of lens fiberprovided by RGD
Primary source: RGD:3090
See related: Ensembl:ENSRNOG00000003132 AllianceGenome:RGD:3090
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as: Aqp0
Summary: Enables water channel activity. Involved in canalicular bile acid transport and water transport. Located in intracellular canaliculus and intracellular membrane-bounded organelle. Human ortholog(s) of this gene implicated in cataract and cataract 15 multiple types. Orthologous to human MIP (major intrinsic protein of lens fiber). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Biased expression in Spleen (RPKM 10.2), Adrenal (RPKM 2.8) and 3 other tissues See more
Orthologs: human mouse all
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Genomic context

Location:: 7q11

Exon count:: 6

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	7	NC_086025.1 (1228089..1237707)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	7	NC_051342.1 (643502..653121)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	7	NC_005106.4 (2635743..2642995)

Chromosome 7 - NC_086025.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

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Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

the kfrs4 rat represents the first characterised rat model with a recessive mutation in the Mip gene
Title: A 5-bp insertion in Mip causes recessive congenital cataract in KFRS4/Kyo rats.
These findings suggest that AQP0 plays an important role in controlling lens ascorbic acid content.
Title: Quantitative analysis of ascorbic acid permeability of aquaporin 0 in the lens.
These results show that changes in the subcellular distribution and processing of AQP0 occur at discrete stages during fiber cell differentiation in the eye lens and suggest that these stages of differentiation.
Title: Differentiation-dependent modification and subcellular distribution of aquaporin-0 suggests multiple functional roles in the rat lens.
These results indicate that serine(235) is required for proper intracellular transport of MIP/AQP0 from the trans-Golgi network to the plasma membrane.
Title: PKC putative phosphorylation site Ser235 is required for MIP/AQP0 translocation to the plasma membrane.
In addition to the lens, aquaporin-0 was expressed in the neural retina, the nuclei and the synaptic terminals of protein kinase alpha- and beta-expressing bipolar and amacrine cells, and by the nuclei of neuronal cells in the ganglion cell layer.
Title: Localization of aquaporin-0 immunoreactivity in the rat retina.
In the testis, AQP-0 expression in the seminiferous epithelium was restricted to Sertoli cells and to Leydig cells
Title: Cell specificity of aquaporins 0, 3, and 10 expressed in the testis, efferent ducts, and epididymis of adult rats.
These results suggest that MIP has pH-dependent ion channel activity.
Title: pH-Dependent channel activity of heterologously-expressed main intrinsic protein (MIP) from rat lens.
Residues around the NPA1 box can influence the folding of the MIP in the membrane, and provide structural evidence for the poor water transport properties of MIP, as the NPA boxes lie outside the plane of the membrane.
Title: Heterologous expression and topography of the main intrinsic protein (MIP) from rat lens.
Expression and localization of aquaporin water channels in rat hepatocytes. Evidence for a role of Aqp0 in canalicular bile secretion.
Title: Expression and localization of aquaporin water channels in rat hepatocytes. Evidence for a role in canalicular bile secretion.

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General gene information

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Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables calmodulin binding	ISS Inferred from Sequence or Structural Similarity more info
enables structural constituent of eye lens	IEA Inferred from Electronic Annotation more info
enables structural constituent of eye lens	ISO Inferred from Sequence Orthology more info
enables water channel activity	IBA Inferred from Biological aspect of Ancestor more info
enables water channel activity	IDA Inferred from Direct Assay more info	PubMed
enables water channel activity	IEA Inferred from Electronic Annotation more info
enables water channel activity	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in canalicular bile acid transport	IDA Inferred from Direct Assay more info	PubMed
involved_in gap junction-mediated intercellular transport	IEA Inferred from Electronic Annotation more info
involved_in gap junction-mediated intercellular transport	ISO Inferred from Sequence Orthology more info
involved_in gap junction-mediated intercellular transport	ISS Inferred from Sequence or Structural Similarity more info
involved_in lens development in camera-type eye	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within lens development in camera-type eye	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of cell adhesion	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of cell adhesion	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of cell adhesion	ISS Inferred from Sequence or Structural Similarity more info
involved_in protein homotetramerization	IEA Inferred from Electronic Annotation more info
involved_in protein homotetramerization	ISO Inferred from Sequence Orthology more info
involved_in protein homotetramerization	ISS Inferred from Sequence or Structural Similarity more info
involved_in transmembrane transport	IEA Inferred from Electronic Annotation more info
involved_in visual perception	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within visual perception	ISO Inferred from Sequence Orthology more info
involved_in water transport	IBA Inferred from Biological aspect of Ancestor more info
involved_in water transport	IDA Inferred from Direct Assay more info	PubMed
involved_in water transport	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within water transport	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
is_active_in apical plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in endoplasmic reticulum	IEA Inferred from Electronic Annotation more info
located_in endoplasmic reticulum	ISO Inferred from Sequence Orthology more info
located_in endoplasmic reticulum	ISS Inferred from Sequence or Structural Similarity more info
located_in gap junction	IEA Inferred from Electronic Annotation more info
located_in intracellular canaliculus	IDA Inferred from Direct Assay more info	PubMed
located_in membrane	ISO Inferred from Sequence Orthology more info
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in plasma membrane	IEA Inferred from Electronic Annotation more info
located_in plasma membrane	ISO Inferred from Sequence Orthology more info
located_in plasma membrane	ISS Inferred from Sequence or Structural Similarity more info

General protein information

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Preferred Names: lens fiber major intrinsic protein

Names: MIP26; MP26; aquaporin-0; major intrinsic protein of eye lens fiber

NP_001099189.1

EC 1.11.1.6

XP_038934393.1

EC 1.11.1.6

XP_038934394.1

EC 1.11.1.6

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001105719.2 → NP_001099189.1 lens fiber major intrinsic protein

See identical proteins and their annotated locations for NP_001099189.1

Status: VALIDATED

Source sequence(s)

JAXUCZ010000007

UniProtKB/Swiss-Prot

P09011

UniProtKB/TrEMBL

A6KSB0, G3V6E0

Related

ENSRNOP00000004223.4, ENSRNOT00000004223.8

Conserved Domains (1) summary

pfam00230
Location:3 → 219

MIP; Major intrinsic protein

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

NC_086025.1 Reference GRCr8

Range

1228089..1237707

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_039078465.2 → XP_038934393.1 lens fiber major intrinsic protein isoform X1

Conserved Domains (1) summary

cl00200
Location:3 → 141

MIP; Major intrinsic protein (MIP) superfamily. Members of the MIP superfamily function as membrane channels that selectively transport water, small neutral molecules, and ions out of and between cells. The channel proteins share a common fold: the N-terminal ...
XM_039078466.2 → XP_038934394.1 lens fiber major intrinsic protein isoform X1

Conserved Domains (1) summary

cl00200
Location:3 → 141

MIP; Major intrinsic protein (MIP) superfamily. Members of the MIP superfamily function as membrane channels that selectively transport water, small neutral molecules, and ions out of and between cells. The channel proteins share a common fold: the N-terminal ...