Chaf1a chromatin assembly factor 1, subunit A [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Chaf1aprovided by MGI
Official Full Name: chromatin assembly factor 1, subunit Aprovided by MGI
Primary source: MGI:MGI:1351331
See related: Ensembl:ENSMUSG00000002835 AllianceGenome:MGI:1351331
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: p150; CAF-1; Cac1p
Summary: Predicted to enable chromo shadow domain binding activity and identical protein binding activity. Predicted to be involved in DNA replication-dependent chromatin assembly and nucleosome assembly. Predicted to act upstream of or within DNA repair and DNA replication. Predicted to be located in chromatin. Predicted to be part of CAF-1 complex. Predicted to be active in nucleus. Human ortholog(s) of this gene implicated in colon cancer; high grade glioma; neuroblastoma; and systemic lupus erythematosus. Orthologous to human CHAF1A (chromatin assembly factor 1 subunit A). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Biased expression in testis adult (RPKM 51.8), CNS E11.5 (RPKM 23.9) and 12 other tissues See more
Orthologs: human all
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Genomic context

Location:: 17 29.13 cM; 17 D

Exon count:: 14

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	17	NC_000083.7 (56347404..56375027)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	17	NC_000083.6 (56040416..56068026)

Chromosome 17 - NC_000083.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

CHAF1A Blocks Neuronal Differentiation and Promotes Neuroblastoma Oncogenesis via Metabolic Reprogramming.
Title: CHAF1A Blocks Neuronal Differentiation and Promotes Neuroblastoma Oncogenesis via Metabolic Reprogramming.
CAF-1 is an essential guardian of the genome in preimplantation mouse embryos.
Title: Histone chaperone CAF-1 mediates repressive histone modifications to protect preimplantation mouse embryos from endogenous retrotransposons.
the histone chaperone CAF-1 to be a novel regulator of somatic cell identity during transcription-factor-induced cell-fate transitions and provide a potential strategy to modulate cellular plasticity in a regenerative setting
Title: The histone chaperone CAF-1 safeguards somatic cell identity.
Findings suggest that p150 may link the DNA-bound Gfi1 repressor complex to histones enabling modifications required for transcriptional silencing.
Title: The p150 subunit of the histone chaperone Caf-1 interacts with the transcriptional repressor Gfi1.
This recruitment is strictly dependent on p150CAF-1, the largest subunit of chromatin assembly factor 1 (CAF-1), and its ability to interact with HP1alpha.
Title: HP1alpha recruitment to DNA damage by p150CAF-1 promotes homologous recombination repair.
Data show that human CAF-1 (p150) contains two PCNA interaction peptides, and suggest that these peptides are part of the mechanism that enables CAF-1 to function behind replication forks without interfering with other PCNA-mediated processes.
Title: Two fundamentally distinct PCNA interaction peptides contribute to chromatin assembly factor 1 function.
findings suggest that CAF-1 is required for the maturation of heterochromatin during preimplantation development and that CAF-1 is specifically required for heterochromatin organization in pluripotent embryonic cells
Title: CAF-1 is essential for heterochromatin organization in pluripotent embryonic cells.
during DNA replication, the interaction of HP1 with p150CAF-1 is essential to promote delivery of HP1 molecules to heterochromatic sites
Title: A CAF-1 dependent pool of HP1 during heterochromatin duplication.

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Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (1)
Targeted (1) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

NoName (e-PCR)
- UniSTS:237670

AU040909 (e-PCR)
- UniSTS:160679

RH125399 (e-PCR)
- UniSTS:162221

RH134524 (e-PCR)
- UniSTS:217804

D5Mit392.1 (e-PCR), detects polymorphism
- UniSTS:130544

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables chromo shadow domain binding	ISO Inferred from Sequence Orthology more info
enables identical protein binding	ISO Inferred from Sequence Orthology more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
acts_upstream_of_or_within DNA damage response	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within DNA repair	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within DNA replication	IEA Inferred from Electronic Annotation more info
involved_in DNA replication-dependent chromatin assembly	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within cell cycle	IEA Inferred from Electronic Annotation more info
involved_in nucleosome assembly	IBA Inferred from Biological aspect of Ancestor more info
involved_in nucleosome assembly	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
part_of CAF-1 complex	IBA Inferred from Biological aspect of Ancestor more info
part_of CAF-1 complex	ISO Inferred from Sequence Orthology more info
part_of chromatin	ISO Inferred from Sequence Orthology more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
part_of protein-containing complex	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: chromatin assembly factor 1 subunit A

Names: CAF-1 subunit A; CAF-I 150 kDa subunit; CAF-I p150; CAF-Ip150; chromatin assembly factor I p150 subunit

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_013733.4 → NP_038761.1 chromatin assembly factor 1 subunit A

See identical proteins and their annotated locations for NP_038761.1

Status: VALIDATED

Source sequence(s)

CT009719

Consensus CDS

CCDS28892.1

UniProtKB/Swiss-Prot

Q3TU22, Q544M2, Q9QWF0

Related

ENSMUSP00000002914.9, ENSMUST00000002914.10

Conserved Domains (4) summary

pfam11600
Location:373 → 458

CAF-1_p150; Chromatin assembly factor 1 complex p150 subunit, N-terminal

pfam12253
Location:537 → 575

CAF1A; Chromatin assembly factor 1 subunit A

pfam15539
Location:644 → 909

CAF1-p150_C2; CAF1 complex subunit p150, region binding to CAF1-p60 at C-term

pfam15557
Location:1 → 210

CAF1-p150_N; CAF1 complex subunit p150, region binding to PCNA