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Spib Spi-B transcription factor (Spi-1/PU.1 related) [ Mus musculus (house mouse) ]

Gene ID: 272382, updated on 9-May-2024

Summary

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Official Symbol
Spibprovided by MGI
Official Full Name
Spi-B transcription factor (Spi-1/PU.1 related)provided by MGI
Primary source
MGI:MGI:892986
See related
Ensembl:ENSMUSG00000008193 AllianceGenome:MGI:892986
Gene type
protein coding
RefSeq status
VALIDATED
Organism
Mus musculus
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as
Spi-B
Summary
Enables DNA-binding transcription factor activity. Involved in immature B cell differentiation. Acts upstream of or within macrophage differentiation and regulation of transcription, DNA-templated. Predicted to be part of transcription regulator complex. Predicted to be active in nucleus. Is expressed in brain and liver. Orthologous to human SPIB (Spi-B transcription factor). [provided by Alliance of Genome Resources, Apr 2022]
Expression
Biased expression in spleen adult (RPKM 101.9), mammary gland adult (RPKM 21.1) and 2 other tissues See more
Orthologs
human all
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Genomic context

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See Spib in Genome Data Viewer
Location:
7 B3; 7 28.83 cM
Exon count:
6
Annotation release Status Assembly Chr Location
RS_2024_02 current GRCm39 (GCF_000001635.27) 7 NC_000073.7 (44175419..44181511, complement)
108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 7 NC_000073.6 (44525995..44532087, complement)

Chromosome 7 - NC_000073.7Genomic Context describing neighboring genes Neighboring gene STARR-seq mESC enhancer starr_18800 Neighboring gene ER membrane protein complex subunit 10 Neighboring gene golgi associated RAB2 interactor 5A Neighboring gene myosin binding protein C, fast-type Neighboring gene STARR-positive B cell enhancer ABC_E10437 Neighboring gene polymerase (DNA directed), delta 1, catalytic subunit Neighboring gene microRNA 7053 Neighboring gene STARR-positive B cell enhancer ABC_E1339 Neighboring gene STARR-positive B cell enhancer ABC_E8170 Neighboring gene STARR-positive B cell enhancer ABC_E2234 Neighboring gene nuclear receptor subfamily 1, group H, member 2

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: Mouse ENCODE transcriptome data
  • Description: RNA profiling data sets generated by the Mouse ENCODE project.
  • BioProject: PRJNA66167
  • Publication: PMID 25409824
  • Analysis date: n/a

Bibliography

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Related articles in PubMed

  1. Mast Cells Are Crucial for Induction of Group 2 Innate Lymphoid Cells and Clearance of Helminth Infections. Shimokawa C, et al. Immunity, 2017 May 16. PMID 28514691
  2. Microfold Cells Actively Translocate Mycobacterium tuberculosis to Initiate Infection. Nair VR, et al. Cell Rep, 2016 Aug 2. PMID 27452467, Free PMC Article
  3. Limitation of immune tolerance-inducing thymic epithelial cell development by Spi-B-mediated negative feedback regulation. Akiyama N, et al. J Exp Med, 2014 Nov 17. PMID 25385757, Free PMC Article
  4. Transcription factor Spi-B-dependent and -independent pathways for the development of Peyer's patch M cells. Sato S, et al. Mucosal Immunol, 2013 Jul. PMID 23212199
  5. Spi-B is critical for plasmacytoid dendritic cell function and development. Sasaki I, et al. Blood, 2012 Dec 6. PMID 23065153

See all (59) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. SpiB regulates the expression of B-cell-related genes and increases the longevity of memory B cells.
    Title: SpiB regulates the expression of B-cell-related genes and increases the longevity of memory B cells.
  2. Opposing Roles for the Related ETS-Family Transcription Factors Spi-B and Spi-C in Regulating B Cell Differentiation and Function.
    Title: Opposing Roles for the Related ETS-Family Transcription Factors Spi-B and Spi-C in Regulating B Cell Differentiation and Function.
  3. The mechanism of action of Spi-B in the transcriptional activation of the interferon-alpha4 gene.
    Title: The mechanism of action of Spi-B in the transcriptional activation of the interferon-α4 gene.
  4. Mice lacking both PU.1 and SpiB in mature B cells do not generate germinal centers and high-affinity antibody after protein immunization. PU.1 and SpiB double-deficient B cells have a survival defect after engagement of CD40 or Toll-like receptors (TLR), despite paradoxically enhanced plasma cell differentiation.
    Title: Environmental sensing by mature B cells is controlled by the transcription factors PU.1 and SpiB.
  5. Mechanistic analysis indicated that E47 activated expression of the transcription factor Spi-B and the suppressor of cytokine signaling 3 (SOCS3), which both downregulated Foxp3 expression. These findings demonstrate that the balance of Id3 and E47 controls the maintenance of Foxp3 expression in Treg cells and, thus, contributes to Treg cell plasticity.
    Title: Id3 Maintains Foxp3 Expression in Regulatory T Cells by Controlling a Transcriptional Network of E47, Spi-B, and SOCS3.
  6. Our results suggest that complementary biological functions of PU.1 and Spi-B may be explained by their interaction with a similar set of regions in the genome of B cells.
    Title: Genome-wide comparison of PU.1 and Spi-B binding sites in a mouse B lymphoma cell line.
  7. Nfkb1 transcriptional activation by PU.1 and Spi-B promotes toll-like receptor-mediated B cell proliferation.
    Title: Nfkb1 activation by the E26 transformation-specific transcription factors PU.1 and Spi-B promotes Toll-like receptor-mediated splenic B cell proliferation.
  8. Data show that transcription factor Spi-B-mediated negative feedback regulation limited medullary thymic epithelial cells (mTECs) development.
    Title: Limitation of immune tolerance-inducing thymic epithelial cell development by Spi-B-mediated negative feedback regulation.
  9. there is a small M-cell population with developmental regulation that is Spi-B independent; however, Spi-B is probably a candidate master regulator of M-cell functional maturation and development by another pathway.
    Title: Transcription factor Spi-B-dependent and -independent pathways for the development of Peyer's patch M cells.
  10. Data indicate that the developmental defects of plasmacytoid dendritic cells (pDC) in Spi-B-deficient mice were more prominent in the bone marrow.
    Title: Spi-B is critical for plasmacytoid dendritic cell function and development.
Submit: New GeneRIF Correction See all GeneRIFs (20)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics  (MGI)
  • Endonuclease-mediated (3) 
  • Targeted (4)  1 citation

General gene information

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Markers

Homology

Gene Ontology Provided by MGI

Function Evidence Code Pubs
enables DNA binding IEA
Inferred from Electronic Annotation
more info
  
enables DNA-binding transcription activator activity, RNA polymerase II-specific ISO
Inferred from Sequence Orthology
more info
  
enables DNA-binding transcription factor activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables DNA-binding transcription factor activity, RNA polymerase II-specific IBA
Inferred from Biological aspect of Ancestor
more info
  
enables RNA polymerase II cis-regulatory region sequence-specific DNA binding ISO
Inferred from Sequence Orthology
more info
  
enables sequence-specific DNA binding IEA
Inferred from Electronic Annotation
more info
  
enables sequence-specific double-stranded DNA binding ISO
Inferred from Sequence Orthology
more info
  
Process Evidence Code Pubs
involved_in cell differentiation IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in immature B cell differentiation IGI
Inferred from Genetic Interaction
more info
 PubMed 
acts_upstream_of_or_within macrophage differentiation IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in positive regulation of transcription by RNA polymerase II ISO
Inferred from Sequence Orthology
more info
  
acts_upstream_of_or_within regulation of DNA-templated transcription IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in regulation of transcription by RNA polymerase II IBA
Inferred from Biological aspect of Ancestor
more info
  
Component Evidence Code Pubs
is_active_in nucleus IBA
Inferred from Biological aspect of Ancestor
more info
  
part_of transcription regulator complex IC
Inferred by Curator
more info
 PubMed 

General protein information

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Preferred Names
transcription factor Spi-B
Names
Ets transcription factor Spi-B
Spi-1/PU.1 related

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_019866.1 → NP_063919.1  transcription factor Spi-B

    See identical proteins and their annotated locations for NP_063919.1

    Status: VALIDATED

    Source sequence(s)
    CAAA01070168
    Consensus CDS
    CCDS21209.1
    UniProtKB/Swiss-Prot
    O35906, O35907, O35909, O55199
    UniProtKB/TrEMBL
    B9EIW0
    Related
    ENSMUSP00000035539.5, ENSMUST00000035323.6
    Conserved Domains (1) summary
    smart00413
    Location:173 → 261
    ETS; erythroblast transformation specific domain

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

  1. NC_000073.7 Reference GRCm39 C57BL/6J

    Range
    44175419..44181511 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_030242628.1 → XP_030098488.1  transcription factor Spi-B isoform X7

    UniProtKB/TrEMBL
    B9EIW0
    Conserved Domains (1) summary
    smart00413
    Location:155 → 243
    ETS; erythroblast transformation specific domain

  2. XM_006540939.3 → XP_006541002.1  transcription factor Spi-B isoform X9

  3. XM_006540933.3 → XP_006540996.1  transcription factor Spi-B isoform X2

    UniProtKB/TrEMBL
    A0A0X1KG64
    Related
    ENSMUSP00000096084.4, ENSMUST00000098483.9
    Conserved Domains (1) summary
    smart00413
    Location:191 → 279
    ETS; erythroblast transformation specific domain

  4. XM_006540938.4 → XP_006541001.1  transcription factor Spi-B isoform X8

  5. XM_006540934.4 → XP_006540997.1  transcription factor Spi-B isoform X4

    UniProtKB/TrEMBL
    A0A0X1KG64
    Conserved Domains (1) summary
    smart00413
    Location:174 → 262
    ETS; erythroblast transformation specific domain

  6. XM_006540932.5 → XP_006540995.1  transcription factor Spi-B isoform X1

    UniProtKB/TrEMBL
    A0A0X1KG64
    Conserved Domains (1) summary
    smart00413
    Location:192 → 280
    ETS; erythroblast transformation specific domain

  7. XM_030242627.2 → XP_030098487.1  transcription factor Spi-B isoform X6

    UniProtKB/TrEMBL
    A0A0U1RPG0
    Conserved Domains (1) summary
    smart00413
    Location:156 → 244
    ETS; erythroblast transformation specific domain

  8. XM_006540935.5 → XP_006540998.1  transcription factor Spi-B isoform X3

    UniProtKB/TrEMBL
    A0A0U1RPG0
    Related
    ENSMUSP00000145968.2, ENSMUST00000205506.2
    Conserved Domains (1) summary
    smart00413
    Location:174 → 262
    ETS; erythroblast transformation specific domain

  9. XM_006540936.4 → XP_006540999.1  transcription factor Spi-B isoform X5

    UniProtKB/TrEMBL
    B9EIW0
    Conserved Domains (1) summary
    smart00413
    Location:164 → 252
    ETS; erythroblast transformation specific domain
Nucleotide Protein Strain
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
O35906.2 GenPept UniProtKB/Swiss-Prot:O35906

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