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Mylk3 myosin light chain kinase 3 [ Rattus norvegicus (Norway rat) ]

Gene ID: 291926, updated on 2-May-2024

Summary

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Official Symbol
Mylk3provided by RGD
Official Full Name
myosin light chain kinase 3provided by RGD
Primary source
RGD:1305801
See related
Ensembl:ENSRNOG00000017546 AllianceGenome:RGD:1305801
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Rattus norvegicus
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as
MLCK; RGD1305801
Summary
Enables calmodulin-dependent protein kinase activity and myosin light chain kinase activity. Involved in myofibril assembly; positive regulation of sarcomere organization; and protein phosphorylation. Located in cytosol. Orthologous to human MYLK3 (myosin light chain kinase 3). [provided by Alliance of Genome Resources, Apr 2022]
Expression
Restricted expression toward (RPKM 1854.5) See more
Orthologs
human mouse all
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Genomic context

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See Mylk3 in Genome Data Viewer
Location:
19q11
Exon count:
15
Annotation release Status Assembly Chr Location
RS_2024_02 current GRCr8 (GCF_036323735.1) 19 NC_086037.1 (37858298..37916805)
RS_2023_06 previous assembly mRatBN7.2 (GCF_015227675.2) 19 NC_051354.1 (21685085..21743587)
106 previous assembly Rnor_6.0 (GCF_000001895.5) 19 NC_005118.4 (27388163..27442451)

Chromosome 19 - NC_086037.1Genomic Context describing neighboring genes Neighboring gene similar to human chromosome 16 open reading frame 87 Neighboring gene ribosomal protein S11, pseudogene 2 Neighboring gene uncharacterized LOC134483311 Neighboring gene origin recognition complex, subunit 6 Neighboring gene U6 spliceosomal RNA Neighboring gene VPS35 retromer complex component

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
  • Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
  • BioProject: PRJNA238328
  • Publication: PMID 24510058
  • Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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Related articles in PubMed

  1. The shift in GH3 cell shape and cell motility is dependent on MLCK and ROCK. Ávila-Rodríguez D, et al. Exp Cell Res, 2017 May 1. PMID 28300565
  2. CaMKII in addition to MLCK contributes to phosphorylation of regulatory light chain in cardiomyocytes. Eikemo H, et al. Biochem Biophys Res Commun, 2016 Feb 26. PMID 26809094
  3. Degradation of cardiac myosin light chain kinase by matrix metalloproteinase-2 contributes to myocardial contractile dysfunction during ischemia/reperfusion. Gao L, et al. J Mol Cell Cardiol, 2014 Dec. PMID 25451385
  4. Mechanism of IL-1beta-induced increase in intestinal epithelial tight junction permeability. Al-Sadi R, et al. J Immunol, 2008 Apr 15. PMID 18390750, Free PMC Article
  5. Identification of cardiac-specific myosin light chain kinase. Chan JY, et al. Circ Res, 2008 Mar 14. PMID 18202317, Free PMC Article

See all (8) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. We found that a rounded shape with high cortical tension depends on MLCK and ROCK, whereas in cells with an elongated shape, MLCK is the primary protein responsible for cell spreading. Further, in cells with a slow and directionally persistent motility, MLCK predominates, while rapid and erratic movement is ROCK-dependent
    Title: The shift in GH3 cell shape and cell motility is dependent on MLCK and ROCK.
  2. In conclusion, in addition to MLCK, CaMKII phosphorylates RLC in cardiomyocytes.
    Title: CaMKII in addition to MLCK contributes to phosphorylation of regulatory light chain in cardiomyocytes.
  3. Ischemia/reperfusion-activated MMP-2 leads to the degradation of cardiac MLCK.
    Title: Degradation of cardiac myosin light chain kinase by matrix metalloproteinase-2 contributes to myocardial contractile dysfunction during ischemia/reperfusion.
Submit: New GeneRIF Correction

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Markers

Homology

Gene Ontology Provided by RGD

Function Evidence Code Pubs
enables ATP binding IEA
Inferred from Electronic Annotation
more info
  
enables calmodulin-dependent protein kinase activity IDA
Inferred from Direct Assay
more info
 PubMed 
NOT enables calmodulin-dependent protein kinase activity ISO
Inferred from Sequence Orthology
more info
  
enables calmodulin-dependent protein kinase activity ISO
Inferred from Sequence Orthology
more info
  
enables myosin light chain kinase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables myosin light chain kinase activity IMP
Inferred from Mutant Phenotype
more info
 PubMed 
enables myosin light chain kinase activity ISO
Inferred from Sequence Orthology
more info
  
enables protein kinase activity IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in cardiac myofibril assembly IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in cardiac myofibril assembly IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in cellular response to interleukin-1 ISO
Inferred from Sequence Orthology
more info
  
involved_in positive regulation of sarcomere organization IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in regulation of vascular permeability involved in acute inflammatory response ISO
Inferred from Sequence Orthology
more info
  
involved_in sarcomere organization IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in sarcomere organization IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in sarcomere organization ISO
Inferred from Sequence Orthology
more info
  
involved_in sarcomerogenesis IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
colocalizes_with actin cytoskeleton IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in actin cytoskeleton ISO
Inferred from Sequence Orthology
more info
  
is_active_in cytoplasm IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in cytoplasm IEA
Inferred from Electronic Annotation
more info
  
located_in cytoplasm ISO
Inferred from Sequence Orthology
more info
  
located_in cytosol IDA
Inferred from Direct Assay
more info
 PubMed 

General protein information

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Preferred Names
myosin light chain kinase 3
Names
cardiac-MLCK
cardiac-MyBP-C-associated Ca/CaM kinase
putative myosin light chain kinase 3
NP_001104280.1
XP_006255417.1
XP_038953507.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001110810.1 → NP_001104280.1  myosin light chain kinase 3

    See identical proteins and their annotated locations for NP_001104280.1

    Status: PROVISIONAL

    Source sequence(s)
    EU236721
    UniProtKB/Swiss-Prot
    A8WE96, E9PT87
    Related
    ENSRNOP00000023657.5, ENSRNOT00000023657.7
    Conserved Domains (2) summary
    smart00220
    Location:485 → 737
    S_TKc; Serine/Threonine protein kinases, catalytic domain
    cd14192
    Location:477 → 737
    STKc_MLCK3; Catalytic domain of the Serine/Threonine Kinase, Myosin Light Chain Kinase 3

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

  1. NC_086037.1 Reference GRCr8

    Range
    37858298..37916805
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_039097579.2 → XP_038953507.1  myosin light chain kinase 3 isoform X2

    Conserved Domains (1) summary
    cd14192
    Location:198 → 458
    STKc_MLCK3; Catalytic domain of the Serine/Threonine Kinase, Myosin Light Chain Kinase 3

  2. XM_006255355.4 → XP_006255417.1  myosin light chain kinase 3 isoform X1

    UniProtKB/TrEMBL
    A0A8I5ZYT9
    Related
    ENSRNOP00000083206.1
    Conserved Domains (2) summary
    smart00220
    Location:352 → 604
    S_TKc; Serine/Threonine protein kinases, catalytic domain
    cd14192
    Location:344 → 604
    STKc_MLCK3; Catalytic domain of the Serine/Threonine Kinase, Myosin Light Chain Kinase 3
Nucleotide Protein Strain
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
E9PT87.1 GenPept UniProtKB/Swiss-Prot:E9PT87

Gene LinkOut

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