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Ncf4 neutrophil cytosolic factor 4 [ Rattus norvegicus (Norway rat) ]

Gene ID: 500904, updated on 5-Mar-2024

Summary

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Official Symbol
Ncf4provided by RGD
Official Full Name
neutrophil cytosolic factor 4provided by RGD
Primary source
RGD:1593157
See related
Ensembl:ENSRNOG00000006940 AllianceGenome:RGD:1593157
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Rattus norvegicus
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Summary
Predicted to enable phosphatidylinositol-3-phosphate binding activity and superoxide-generating NADPH oxidase activator activity. Predicted to be involved in superoxide anion generation. Predicted to be located in cytosol and endosome membrane. Predicted to be part of NADPH oxidase complex. Predicted to be active in cytoplasm. Human ortholog(s) of this gene implicated in autosomal recessive chronic granulomatous disease cytochrome b-positive type III and rheumatoid arthritis. Orthologous to human NCF4 (neutrophil cytosolic factor 4). [provided by Alliance of Genome Resources, Apr 2022]
Expression
Biased expression in Spleen (RPKM 99.4), Lung (RPKM 35.6) and 7 other tissues See more
Orthologs
human mouse all
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Genomic context

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Location:
7q34
Exon count:
11
Annotation release Status Assembly Chr Location
RS_2024_02 current GRCr8 (GCF_036323735.1) 7 NC_086025.1 (111705926..111723893)
RS_2023_06 previous assembly mRatBN7.2 (GCF_015227675.2) 7 NC_051342.1 (109825420..109843389)
106 previous assembly Rnor_6.0 (GCF_000001895.5) 7 NC_005106.4 (119481711..119499426)

Chromosome 7 - NC_086025.1Genomic Context describing neighboring genes Neighboring gene intraflagellar transport 27 Neighboring gene parvalbumin Neighboring gene colony stimulating factor 2 receptor subunit beta Neighboring gene ciliary microtubule inner protein 4

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
  • Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
  • BioProject: PRJNA238328
  • Publication: PMID 24510058
  • Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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Related articles in PubMed

  1. The crystal structure of the PX domain from p40(phox) bound to phosphatidylinositol 3-phosphate. Bravo J, et al. Mol Cell, 2001 Oct. PMID 11684018
  2. A case-control study of rheumatoid arthritis identifies an associated single nucleotide polymorphism in the NCF4 gene, supporting a role for the NADPH-oxidase complex in autoimmunity. Olsson LM, et al. Arthritis Res Ther, 2007. PMID 17897462, Free PMC Article
  3. A time course of NADPH-oxidase up-regulation and endothelial nitric oxide synthase activation in the hippocampus following neurotrauma. Ansari MA, et al. Free Radic Biol Med, 2014 Dec. PMID 25224032, Free PMC Article
  4. p40phox, a third cytosolic component of the activation complex of the NADPH oxidase to contain src homology 3 domains. Wientjes FB, et al. Biochem J, 1993 Dec 15. PMID 8280052, Free PMC Article
  5. Identification of non-mitochondrial NADPH oxidase and the spatio-temporal organization of its components in mouse spermatozoa. Shukla S, et al. Biochem Biophys Res Commun, 2005 Jun 3. PMID 15850784

See all (7) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. The levels of cytosolic p67Phox, p47Phox, and p40Phox were increased in membrane fractions following traumatic brain injury.
    Title: A time course of NADPH-oxidase up-regulation and endothelial nitric oxide synthase activation in the hippocampus following neurotrauma.
Submit: New GeneRIF Correction

General gene information

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Markers

Homology

Gene Ontology Provided by RGD

Function Evidence Code Pubs
enables phosphatidylinositol-3-phosphate binding ISO
Inferred from Sequence Orthology
more info
  
enables superoxide-generating NADPH oxidase activator activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables superoxide-generating NADPH oxidase activator activity ISO
Inferred from Sequence Orthology
more info
  
Process Evidence Code Pubs
involved_in phagocytosis IEA
Inferred from Electronic Annotation
more info
  
involved_in respiratory burst IEA
Inferred from Electronic Annotation
more info
  
involved_in superoxide anion generation IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in superoxide anion generation ISO
Inferred from Sequence Orthology
more info
  
Component Evidence Code Pubs
part_of NADPH oxidase complex IBA
Inferred from Biological aspect of Ancestor
more info
  
part_of NADPH oxidase complex ISO
Inferred from Sequence Orthology
more info
  
is_active_in cytoplasm IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in cytoplasm ISO
Inferred from Sequence Orthology
more info
  
located_in cytosol ISO
Inferred from Sequence Orthology
more info
  
located_in endosome membrane ISO
Inferred from Sequence Orthology
more info
  
located_in membrane ISO
Inferred from Sequence Orthology
more info
  

General protein information

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Preferred Names
neutrophil cytosol factor 4

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001127304.1 → NP_001120776.1  neutrophil cytosol factor 4

    See identical proteins and their annotated locations for NP_001120776.1

    Status: PROVISIONAL

    Source sequence(s)
    CH473950
    UniProtKB/TrEMBL
    A0A8I5ZNF3, A0A9K3Y7E3, B2RZ98, D3ZS40
    Related
    ENSRNOP00000009544.6, ENSRNOT00000009544.7
    Conserved Domains (3) summary
    cd06399
    Location:238 → 329
    PB1_P40; The PB1 domain is essential part of the p40 adaptor protein which plays an important role in activating phagocyte NADPH oxidase during phagocytosis. The PB1 domain is a modular domain mediating specific protein-protein interaction which play a role in ...
    cd06882
    Location:19 → 141
    PX_p40phox; The phosphoinositide binding Phox Homology domain of the p40phox subunit of NADPH oxidase
    cd11869
    Location:174 → 227
    SH3_p40phox; Src Homology 3 domain of the p40phox subunit of NADPH oxidase

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

  1. NC_086025.1 Reference GRCr8

    Range
    111705926..111723893
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_006241939.5 → XP_006242001.1  neutrophil cytosol factor 4 isoform X1

    UniProtKB/TrEMBL
    A0A8I5ZNF3
    Conserved Domains (3) summary
    cd06399
    Location:254 → 345
    PB1_P40; The PB1 domain is essential part of the p40 adaptor protein which plays an important role in activating phagocyte NADPH oxidase during phagocytosis. The PB1 domain is a modular domain mediating specific protein-protein interaction which play a role in ...
    cd06882
    Location:56 → 157
    PX_p40phox; The phosphoinositide binding Phox Homology domain of the p40phox subunit of NADPH oxidase
    cd11869
    Location:190 → 243
    SH3_p40phox; Src Homology 3 domain of the p40phox subunit of NADPH oxidase
Nucleotide Protein Strain
Heading Accession and Version

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