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TOP2B DNA topoisomerase II beta [ Homo sapiens (human) ]

Gene ID: 7155, updated on 4-Jan-2025

Summary

Official Symbol
TOP2Bprovided by HGNC
Official Full Name
DNA topoisomerase II betaprovided by HGNC
Primary source
HGNC:HGNC:11990
See related
Ensembl:ENSG00000077097 MIM:126431; AllianceGenome:HGNC:11990
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Homo sapiens
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as
BILU; TOPIIB; top2beta
Summary
This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, beta, is localized to chromosome 3 and the alpha form is localized to chromosome 17. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2016]
Expression
Ubiquitous expression in urinary bladder (RPKM 32.3), brain (RPKM 31.7) and 25 other tissues See more
Orthologs
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Genomic context

See TOP2B in Genome Data Viewer
Location:
3p24.2
Exon count:
36
Annotation release Status Assembly Chr Location
RS_2024_08 current GRCh38.p14 (GCF_000001405.40) 3 NC_000003.12 (25597905..25664907, complement)
RS_2024_08 current T2T-CHM13v2.0 (GCF_009914755.1) 3 NC_060927.1 (25600752..25667754, complement)
RS_2024_09 previous assembly GRCh37.p13 (GCF_000001405.25) 3 NC_000003.11 (25639396..25706398, complement)

Chromosome 3 - NC_000003.12Genomic Context describing neighboring genes Neighboring gene retinoic acid receptor beta Neighboring gene H3K4me1 hESC enhancer GRCh37_chr3:25491906-25492406 Neighboring gene NANOG-H3K27ac hESC enhancer GRCh37_chr3:25495987-25496611 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr3:25496710-25497250 Neighboring gene uncharacterized LOC124909356 Neighboring gene H3 histone pseudogene 10 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr3:25609805-25610306 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr3:25614420-25614948 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr3:25614949-25615476 Neighboring gene NUP98-TOP2B recombination region Neighboring gene ATAC-STARR-seq lymphoblastoid active region 19608 Neighboring gene ReSE screen-validated silencer GRCh37_chr3:25705465-25705653 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 14146 Neighboring gene H3K27ac hESC enhancer GRCh37_chr3:25706135-25706698 Neighboring gene microRNA 4442 Neighboring gene cysteine rich protein 1 pseudogene 2 Neighboring gene ribosomal protein L32 pseudogene 11 Neighboring gene N-glycanase 1

Genomic regions, transcripts, and products

Expression

  • Project title: Tissue-specific circular RNA induction during human fetal development
  • Description: 35 human fetal samples from 6 tissues (3 - 7 replicates per tissue) collected between 10 and 20 weeks gestational time were sequenced using Illumina TruSeq Stranded Total RNA
  • BioProject: PRJNA270632
  • Publication: PMID 26076956
  • Analysis date: Mon Apr 2 22:54:59 2018

Bibliography

GeneRIFs: Gene References Into Functions

What's a GeneRIF?

HIV-1 interactions

Replication interactions

Interaction Pubs
HIV-1 replication is enhanced by over-expression of TOP2B (TopoisomeraseIIb) PubMed
HIV-1 replication requires TOP2B (TopoisomeraseIIb) as shown through inhibition by ICRF-193 or merbarone treatment or siRNA knockdown PubMed
Knockdown of TOP2A, TOP2B, or TOP2A/2B by siRNA significantly decreases HIV-1 replication in Sup T1 cells PubMed

Protein interactions

Protein Gene Interaction Pubs
Tat tat HIV-1 Tat binds TOP2B (topoisomeraseIIb), XRCC6 (Ku70), and PARP1 during transactivation and viral replication PubMed
capsid gag Knockdown of TOP2A, TOP2B, or TOP2A/2B by siRNA significantly decreases p24 levels in HIV-1 infected cell culture supernatants when compared to control PubMed
reverse transcriptase gag-pol HIV-1 RT co-localizes with TOP2A and TOP2B in cytosol of HIV-1 infected Sup T1 and PBMC cells PubMed

Go to the HIV-1, Human Interaction Database

Pathways from PubChem

Interactions

General gene information

Gene Ontology Provided by GOA

Component Evidence Code Pubs
located_in cytosol PubMed 
colocalizes_with heterochromatin PubMed 
NOT located_in nucleolus PubMed 
located_in nucleolus PubMed 
located_in nucleoplasm PubMed 
located_in nucleoplasm  
is_active_in nucleus  
located_in nucleus PubMed 
part_of ribonucleoprotein complex  

General protein information

Preferred Names
DNA topoisomerase 2-beta
Names
DNA topoisomerase II, 180 kD
DNA topoisomerase II, beta isozyme
U937 associated antigen
antigen MLAA-44
topo II beta
topoisomerase (DNA) II beta 180kDa
topoisomerase II beta
topoisomerase IIb
NP_001059.2
NP_001317629.1
XP_011532359.1
XP_047304777.1
XP_047304778.1
XP_054203683.1
XP_054203684.1
XP_054203685.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

Genomic

  1. NG_052961.1 RefSeqGene

    Range
    4466..71389
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_001068.3NP_001059.2  DNA topoisomerase 2-beta isoform 2

    See identical proteins and their annotated locations for NP_001059.2

    Status: REVIEWED

    Description
    Transcript Variant: This variant (2) contains an alternate in-frame splice site in the 5' coding region, compared to variant 1. It encodes isoform 2, which is shorter than isoform 1.
    Source sequence(s)
    AB208879, BF514624, BM150930, X68060
    Consensus CDS
    CCDS46776.1
    UniProtKB/TrEMBL
    Q71UH4
    Related
    ENSP00000396704.2, ENST00000435706.6
    Conserved Domains (7) summary
    cd03365
    Location:471591
    TOPRIM_TopoIIA; TOPRIM_TopoIIA: topoisomerase-primase (TOPRIM) nucleotidyl transferase/hydrolase domain of the type found in proteins of the type IIA family of DNA topoisomerases similar to Saccharomyces cerevisiae Topoisomerase II. TopoIIA enzymes cut both strands of ...
    cd00187
    Location:7271187
    TOP4c; DNA Topoisomerase, subtype IIA; domain A'; bacterial DNA topoisomerase IV (C subunit, ParC), bacterial DNA gyrases (A subunit, GyrA),mammalian DNA toposiomerases II. DNA topoisomerases are essential enzymes that regulate the conformational changes in DNA ...
    cd03481
    Location:281433
    TopoIIA_Trans_ScTopoIIA; TopoIIA_Trans_ScTopoIIA: Transducer domain, having a ribosomal S5 domain 2-like fold, of the type found in proteins of the type IIA family of DNA topoisomerases similar to Saccharomyces cerevisiae Topo IIA. S. cerevisiae Topo IIA is a homodimer encoded ...
    PTZ00108
    Location:411466
    PTZ00108; DNA topoisomerase 2-like protein; Provisional
    pfam02518
    Location:96193
    HATPase_c; Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase
    pfam08070
    Location:15031604
    DTHCT; DTHCT (NUC029) region
    pfam16898
    Location:589727
    TOPRIM_C; C-terminal associated domain of TOPRIM
  2. NM_001330700.2NP_001317629.1  DNA topoisomerase 2-beta isoform 1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).
    Source sequence(s)
    AC093416
    Consensus CDS
    CCDS82746.1
    UniProtKB/Swiss-Prot
    Q02880, Q13600, Q9UMG8, Q9UQP8
    UniProtKB/TrEMBL
    Q71UH4
    Related
    ENSP00000264331.4, ENST00000264331.9

RefSeqs of Annotated Genomes: GCF_000001405.40-RS_2024_08

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38.p14 Primary Assembly

Genomic

  1. NC_000003.12 Reference GRCh38.p14 Primary Assembly

    Range
    25597905..25664907 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_047448821.1XP_047304777.1  DNA topoisomerase 2-beta isoform X2

    UniProtKB/TrEMBL
    Q71UH4
    Related
    ENSP00000514098.1, ENST00000699043.1
  2. XM_011534057.4XP_011532359.1  DNA topoisomerase 2-beta isoform X1

    UniProtKB/TrEMBL
    Q71UH4
    Conserved Domains (7) summary
    cd03365
    Location:476596
    TOPRIM_TopoIIA; TOPRIM_TopoIIA: topoisomerase-primase (TOPRIM) nucleotidyl transferase/hydrolase domain of the type found in proteins of the type IIA family of DNA topoisomerases similar to Saccharomyces cerevisiae Topoisomerase II. TopoIIA enzymes cut both strands of ...
    cd00187
    Location:7321192
    TOP4c; DNA Topoisomerase, subtype IIA; domain A'; bacterial DNA topoisomerase IV (C subunit, ParC), bacterial DNA gyrases (A subunit, GyrA),mammalian DNA toposiomerases II. DNA topoisomerases are essential enzymes that regulate the conformational changes in DNA ...
    cd03481
    Location:286438
    TopoIIA_Trans_ScTopoIIA; TopoIIA_Trans_ScTopoIIA: Transducer domain, having a ribosomal S5 domain 2-like fold, of the type found in proteins of the type IIA family of DNA topoisomerases similar to Saccharomyces cerevisiae Topo IIA. S. cerevisiae Topo IIA is a homodimer encoded ...
    PTZ00108
    Location:461466
    PTZ00108; DNA topoisomerase 2-like protein; Provisional
    pfam02518
    Location:101198
    HATPase_c; Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase
    pfam08070
    Location:14711572
    DTHCT; DTHCT (NUC029) region
    pfam16898
    Location:594732
    TOPRIM_C; C-terminal associated domain of TOPRIM
  3. XM_047448822.1XP_047304778.1  DNA topoisomerase 2-beta isoform X3

    UniProtKB/TrEMBL
    E9PCY5
    Related
    ENSP00000391112.1, ENST00000424225.1

Alternate T2T-CHM13v2.0

Genomic

  1. NC_060927.1 Alternate T2T-CHM13v2.0

    Range
    25600752..25667754 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_054347709.1XP_054203684.1  DNA topoisomerase 2-beta isoform X2

    UniProtKB/TrEMBL
    Q71UH4
  2. XM_054347708.1XP_054203683.1  DNA topoisomerase 2-beta isoform X1

    UniProtKB/TrEMBL
    Q71UH4
  3. XM_054347710.1XP_054203685.1  DNA topoisomerase 2-beta isoform X3

    UniProtKB/TrEMBL
    E9PCY5
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