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NHA1 Nha1p [ Saccharomyces cerevisiae S288C ]

Gene ID: 850829, updated on 13-Apr-2024

Summary

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Gene symbol
NHA1
Gene description
Nha1p
Primary source
SGD:S000004128
Locus tag
YLR138W
See related
AllianceGenome:SGD:S000004128
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Saccharomyces cerevisiae S288C (strain: S288C)
Lineage
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary
Enables sodium:proton antiporter activity. Involved in cellular potassium ion homeostasis; metal ion export; and response to osmotic stress. Located in membrane raft and plasma membrane. [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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Location:
chromosome: XII
Exon count:
1
Sequence:
Chromosome: XII; NC_001144.5 (418437..421394)

Chromosome XII - NC_001144.5Genomic Context describing neighboring genes Neighboring gene Tis11p Neighboring gene S-adenosylmethionine-dependent methyltransferase Neighboring gene Sls1p Neighboring gene Rrn5p

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_001144.5

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Saccharomyces cerevisiae BY4741 and W303-1A laboratory strains differ in salt tolerance. Petrezselyova S, et al. Fungal Biol, 2010 Feb-Mar. PMID 20960970
  2. Functional comparison of plasma-membrane Na+/H+ antiporters from two pathogenic Candida species. Krauke Y, et al. BMC Microbiol, 2008 May 20. PMID 18492255, Free PMC Article
  3. Heterologous expression of Na+/H+ antiporter gene (CvNHA1) from salt-tolerant yeast Candida versatilis in Saccharomyces cerevisiae Na+-transporter deficient mutants. Watanabe Y, et al. Biosci Biotechnol Biochem, 2008 Apr. PMID 18391463
  4. Oligomerization of the Saccharomyces cerevisiae Na+/H+ antiporter Nha1p: implications for its antiporter activity. Mitsui K, et al. Biochim Biophys Acta, 2005 Dec 30. PMID 16360116
  5. Identification of conserved prolyl residue important for transport activity and the substrate specificity range of yeast plasma membrane Na+/H+ antiporters. Kinclova-Zimmermannova O, et al. J Biol Chem, 2005 Aug 26. PMID 15994323

See all (81) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. C5 conserved region of hydrophilic C-terminal part of Saccharomyces cerevisiae Nha1 antiporter determines its requirement of Erv14 COPII cargo receptor for plasma-membrane targeting.
    Title: C5 conserved region of hydrophilic C-terminal part of Saccharomyces cerevisiae Nha1 antiporter determines its requirement of Erv14 COPII cargo receptor for plasma-membrane targeting.
  2. The 14-3-3 binding induces a disorder-to-order transition of the C-terminus of Nha1, and in vivo experiments showed that the mutation of Ser481 to Ala significantly increases cation efflux activity via Nha1, which renders cells sensitive to low K(+) concentrations.
    Title: The activity of Saccharomyces cerevisiae Na(+), K(+)/H(+) antiporter Nha1 is negatively regulated by 14-3-3 protein binding at serine 481.
  3. Erv14 cargo receptor participates in yeast salt tolerance via its interaction with the plasma-membrane Nha1 cation/proton antiporter.
    Title: Erv14 cargo receptor participates in yeast salt tolerance via its interaction with the plasma-membrane Nha1 cation/proton antiporter.
  4. All three potassium-exporting systems the Nha1 antiporter, Ena ATPase and Tok1 channel contribute to potassium homeostasis and are active upon potassium limitation in wild-type cells.
    Title: Plasma-membrane hyperpolarization diminishes the cation efflux via Nha1 antiporter and Ena ATPase under potassium-limiting conditions.
  5. sphingolipids are required for the stable localization of Nha1p to the plasma membrane
    Title: Saccharomyces cerevisiae Na+/H+ antiporter Nha1p associates with lipid rafts and requires sphingolipid for stable localization to the plasma membrane.
  6. results support the notion that Nha1p exists in membranes as a dimer and that the interaction of its monomers is important for its antiporter activity
    Title: Oligomerization of the Saccharomyces cerevisiae Na+/H+ antiporter Nha1p: implications for its antiporter activity.
  7. A detailed molecular mechanism for the Na+/H+ antiporter NHA1 is demonstrated, and the functional importance of domains proximal to the membrane region is discussed.
    Title: A conserved domain in the tail region of the Saccharomyces cerevisiae Na+/H+ antiporter (Nha1p) plays important roles in localization and salinity-resistant cell-growth.
  8. The Na+,K+/H+ -antiporter Nha1 influences the plasma membrane potential of Saccharomyces cerevisiae.
    Title: The Na+,K+/H+ -antiporter Nha1 influences the plasma membrane potential of Saccharomyces cerevisiae.
  9. Nha1p and its C-terminus in the immediate response to hyperosmotic shock as part of the rapid rescue mechanism.
    Title: Functional study of the Nha1p C-terminus: involvement in cell response to changes in external osmolarity.
  10. analysis of Nha1p transport activity using isolated secretory vesicles
    Title: Characterization of the ion transport activity of the budding yeast Na+/H+ antiporter, Nha1p, using isolated secretory vesicles.
Submit: New GeneRIF Correction See all GeneRIFs (11)

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Homology

Gene Ontology Provided by SGD

Function Evidence Code Pubs
enables antiporter activity IEA
Inferred from Electronic Annotation
more info
  
enables potassium ion transmembrane transporter activity IEA
Inferred from Electronic Annotation
more info
  
enables sodium:proton antiporter activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables sodium:proton antiporter activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables sodium:proton antiporter activity IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in intracellular monoatomic cation homeostasis IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in intracellular potassium ion homeostasis IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in intracellular potassium ion homeostasis IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in potassium ion export across plasma membrane IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in proton export across plasma membrane IEA
Inferred from Electronic Annotation
more info
  
involved_in regulation of membrane potential IEA
Inferred from Electronic Annotation
more info
  
involved_in response to osmotic stress IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in sodium ion export across plasma membrane IEA
Inferred from Electronic Annotation
more info
  
involved_in sodium ion export across plasma membrane IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in sodium ion transmembrane transport IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in sodium ion transport IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
located_in endoplasmic reticulum HDA PubMed 
located_in membrane raft IDA
Inferred from Direct Assay
more info
 PubMed 
is_active_in plasma membrane IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in plasma membrane IDA
Inferred from Direct Assay
more info
 PubMed 
located_in plasma membrane IEA
Inferred from Electronic Annotation
more info
  

General protein information

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Preferred Names
Nha1p
NP_013239.1
  • Na+/H+ antiporter; involved in sodium and potassium efflux through the plasma membrane; required for alkali cation tolerance at acidic pH

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_001144.5 Reference assembly

    Range
    418437..421394
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_001182025.1 → NP_013239.1  TPA: Nha1p [Saccharomyces cerevisiae S288C]

    See identical proteins and their annotated locations for NP_013239.1

    Status: REVIEWED

    UniProtKB/Swiss-Prot
    D6VYD3, Q99271
    UniProtKB/TrEMBL
    A0A6A5PR75, A7A137, B3LT99, C7GMJ0, C8ZDA7, N1NZB6
    Conserved Domains (1) summary
    TIGR00844
    Location:2 → 923
    c_cpa1; na(+)/h(+) antiporter
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q99271.1

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