INO1 inositol-3-phosphate synthase INO1 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Gene symbol: INO1
Gene description: inositol-3-phosphate synthase INO1
Primary source: FungiDB:YJL153C
Locus tag: YJL153C
See related: SGD:S000003689; AllianceGenome:SGD:S000003689
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: APR1
Summary: Enables inositol-3-phosphate synthase activity. Involved in inositol biosynthetic process. Located in cytoplasm. Orthologous to human ISYNA1 (inositol-3-phosphate synthase 1). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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Location:: chromosome: X

Exon count:: 1

Sequence:: Chromosome: X; NC_001142.9 (134332..135933, complement)

Chromosome X - NC_001142.9 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001142.9

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Data suggest that a specific pool of phosphatidic acid, associated with lipid droplet formation in perinuclear ER, is responsible for initial rapid exit of Opi1p from nucleus to ER and is required for Ino1 expression in presence of choline; interaction between Opi1p and Scs2p in ER membrane facilitates transit of phosphatidic acid from ER to mitochondria.
Title: Interaction between repressor Opi1p and ER membrane protein Scs2p facilitates transit of phosphatidic acid from the ER to mitochondria and is essential for INO1 gene expression in the presence of choline.
Transcriptional memory of INO1 requires a remodeled form of the Set1/COMPASS methyltransferase lacking Spp1, which dimethylates histone H3 lysine 4 (H3K4me2).
Title: Set1/COMPASS and Mediator are repurposed to promote epigenetic transcriptional memory.
analysis of how myo-inositol-3-phosphate synthase is regulated by phosphorylation
Title: Phosphorylation regulates myo-inositol-3-phosphate synthase: a novel regulatory mechanism of inositol biosynthesis.
While the pattern of regulation of these two genes was similar, results showed that intermediate levels of inositol repressed INO1 and induced SNA3.
Title: Transcription regulation of a yeast gene from a downstream location.
Data show that the introns have a direct role in enhancing transcription of INO1 and ASC1 because there was a marked increase in nascent transcripts from these genes in the presence of an intron.
Title: Role for gene looping in intron-mediated enhancement of transcription.
these data provide a mechanism for cross talk within transcriptional co-activators Ino80p and Snf2p during INO1 activation.
Title: INO1 induction requires chromatin remodelers Ino80p and Snf2p but not the histone acetylases.
Cbf1p binding to the upstream sites was required for the binding of the ISW2 chromatin-remodeling complex to the Ino2p-Ino4p-binding sites on the INO1 promoter.
Title: Derepression of INO1 transcription requires cooperation between the Ino2p-Ino4p heterodimer and Cbf1p and recruitment of the ISW2 chromatin-remodeling complex.
Interaction of a DNA zip code with the nuclear pore complex promotes H2A.Z incorporation and INO1 transcriptional memory.
Title: Interaction of a DNA zip code with the nuclear pore complex promotes H2A.Z incorporation and INO1 transcriptional memory.
Induction of INO1 resulted in acetylation of both histones H3 and H4 at the INO1 promoter and sequences farther downstream in the coding region, suggesting a gene-wide acetylation in response to transcriptional activation.
Title: Gene-wide histone acetylation at the yeast INO1 requires the transcriptional activator Ino2p.
Full transcriptional activation of INO1 and another GRS-containing gene requires GRS I (gene recruitment sequences) mediated targeting of the promoter to the nuclear periphery.
Title: DNA zip codes control an ancient mechanism for gene targeting to the nuclear periphery.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables inositol-3-phosphate synthase activity	IBA Inferred from Biological aspect of Ancestor more info
enables inositol-3-phosphate synthase activity	IDA Inferred from Direct Assay more info	PubMed
enables inositol-3-phosphate synthase activity	IEA Inferred from Electronic Annotation more info
enables isomerase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in inositol biosynthetic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in inositol biosynthetic process	IEA Inferred from Electronic Annotation more info
involved_in inositol biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in phospholipid biosynthetic process	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in cytoplasm	HDA more info	PubMed
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: inositol-3-phosphate synthase INO1

NP_012382.2

EC 5.5.1.4

Inositol-3-phosphate synthase; involved in synthesis of inositol phosphates and inositol-containing phospholipids; transcription is coregulated with other phospholipid biosynthetic genes by Ino2p and Ino4p, which bind the UASINO DNA element

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001142.9 Reference assembly

Range

134332..135933 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001181586.1 → NP_012382.2 TPA: inositol-3-phosphate synthase INO1 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_012382.2

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VW34, P11986

UniProtKB/TrEMBL

A0A8H4C0U2, A6ZQH9, B3LPW8, C7GPY0, C8ZB67, N1P1Z0

Conserved Domains (1) summary

pfam07994
Location:66 → 515

NAD_binding_5; Myo-inositol-1-phosphate synthase