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RIB2 bifunctional DRAP deaminase/tRNA pseudouridine synthase RIB2 [ Saccharomyces cerevisiae S288C ]

Gene ID: 854088, updated on 13-Apr-2024

Summary

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Gene symbol
RIB2
Gene description
bifunctional DRAP deaminase/tRNA pseudouridine synthase RIB2
Primary source
SGD:S000005427
Locus tag
YOL066C
See related
AllianceGenome:SGD:S000005427
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Saccharomyces cerevisiae S288C (strain: S288C)
Lineage
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as
PUS8
Summary
Enables 2,5-diamino-6-ribitylamino-4(3H)-pyrimidinone 5'-phosphate deaminase activity and pseudouridine synthase activity. Involved in riboflavin biosynthetic process and tRNA pseudouridine synthesis. Located in cytoplasm. Orthologous to human RPUSD2 (RNA pseudouridine synthase domain containing 2). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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Location:
chromosome: XV
Exon count:
1
Sequence:
Chromosome: XV; NC_001147.6 (202696..204471, complement)

Chromosome XV - NC_001147.6Genomic Context describing neighboring genes Neighboring gene histone deacetylase HST1 Neighboring gene Rtg1p Neighboring gene phosphoinositide 5-phosphatase INP54 Neighboring gene 3'(2'),5'-bisphosphate nucleotidase

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_001147.6

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Optimisation of expression and purification of the recombinant Yol066 (Rib2) protein from Saccharomyces cerevisiae. Urban A, et al. J Chromatogr B Analyt Technol Biomed Life Sci, 2003 Mar 25. PMID 12651014
  2. Biosynthesis of riboflavin. Formation of 2,5-diamino-6-hydroxy-4-(1'-D-ribitylamino)pyrimidine in a riboflavin auxotroph. Bacher A, et al. J Biol Chem, 1970 Sep 25. PMID 5456141
  3. Evolution of vitamin B2 biosynthesis: eubacterial RibG and fungal Rib2 deaminases. Chen SC, et al. Acta Crystallogr D Biol Crystallogr, 2013 Feb. PMID 23385458
  4. Pseudouridylation at position 32 of mitochondrial and cytoplasmic tRNAs requires two distinct enzymes in Saccharomyces cerevisiae. Behm-Ansmant I, et al. J Biol Chem, 2004 Dec 17. PMID 15466869
  5. Biosynthesis of riboflavine in Saccharomyces cerevisiae: the role of genes rib 1 and rib 7 . Oltmanns O, et al. J Bacteriol, 1972 Jun. PMID 4555411, Free PMC Article

See all (28) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. two enzymes, Rib2/Pus8p and Pus9p, are required for Psi32 formation in cytoplasmic and mitochondrial tRNAs, respectively
    Title: Pseudouridylation at position 32 of mitochondrial and cytoplasmic tRNAs requires two distinct enzymes in Saccharomyces cerevisiae.
Submit: New GeneRIF Correction

Pathways from PubChem

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Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Homology

Gene Ontology Provided by SGD

Function Evidence Code Pubs
enables RNA binding IEA
Inferred from Electronic Annotation
more info
  
enables diaminohydroxyphosphoribosylaminopyrimidine deaminase activity IEA
Inferred from Electronic Annotation
more info
  
enables diaminohydroxyphosphoribosylaminopyrimidine deaminase activity IMP
Inferred from Mutant Phenotype
more info
 PubMed 
enables hydrolase activity IEA
Inferred from Electronic Annotation
more info
  
enables isomerase activity IEA
Inferred from Electronic Annotation
more info
  
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
enables pseudouridine synthase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables pseudouridine synthase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables pseudouridine synthase activity IEA
Inferred from Electronic Annotation
more info
  
enables pseudouridine synthase activity IMP
Inferred from Mutant Phenotype
more info
 PubMed 
enables tRNA pseudouridine(32) synthase activity IEA
Inferred from Electronic Annotation
more info
  
enables zinc ion binding RCA
inferred from Reviewed Computational Analysis
more info
 PubMed 
Process Evidence Code Pubs
involved_in enzyme-directed rRNA pseudouridine synthesis IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in nucleobase-containing compound metabolic process IEA
Inferred from Electronic Annotation
more info
  
involved_in pseudouridine synthesis IEA
Inferred from Electronic Annotation
more info
  
involved_in riboflavin biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
involved_in riboflavin biosynthetic process IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in tRNA processing IEA
Inferred from Electronic Annotation
more info
  
involved_in tRNA pseudouridine synthesis IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in tRNA pseudouridine synthesis IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
located_in cytoplasm HDA PubMed 
located_in cytoplasm IEA
Inferred from Electronic Annotation
more info
  

General protein information

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Preferred Names
bifunctional DRAP deaminase/tRNA pseudouridine synthase RIB2
NP_014575.1
  • Bifunctional DRAP deaminase tRNA:pseudouridine synthase; the deaminase catalyzes the third step in riboflavin biosynthesis and the synthase catalyzes formation of pseudouridine at position 32 in cytoplasmic tRNAs; RIB2 has a paralog, PUS9, that arose from the whole genome duplication

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_001147.6 Reference assembly

    Range
    202696..204471 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_001183321.1 → NP_014575.1  TPA: bifunctional DRAP deaminase/tRNA pseudouridine synthase RIB2 [Saccharomyces cerevisiae S288C]

    See identical proteins and their annotated locations for NP_014575.1

    Status: REVIEWED

    UniProtKB/Swiss-Prot
    D6W201, Q12362
    UniProtKB/TrEMBL
    A0A8H4BWM6, A6ZNE4, B3LIZ4, C7GK33, N1NZ56
    Conserved Domains (2) summary
    TIGR00005
    Location:94 → 406
    rluA_subfam; pseudouridine synthase, RluA family
    pfam18785
    Location:459 → 580
    Inv-AAD; Invertebrate-AID/APOBEC-deaminase
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q12362.1

Gene LinkOut

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