SUA7 transcription factor TFIIB [Saccharomyces cerevisiae S288C] - Gene

Summary

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Gene symbol: SUA7
Gene description: transcription factor TFIIB
Primary source: SGD:S000006290
Locus tag: YPR086W
See related: AllianceGenome:SGD:S000006290
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: SOH4
Summary: Enables RNA polymerase II complex recruiting activity and TBP-class protein binding activity. Involved in positive regulation of transcription regulatory region DNA binding activity and transcription initiation from RNA polymerase II promoter. Located in nucleus. Part of transcription preinitiation complex. Orthologous to human GTF2B (general transcription factor IIB). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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Location:: chromosome: XVI

Exon count:: 1

Sequence:: Chromosome: XVI; NC_001148.4 (710101..711138)

Chromosome XVI - NC_001148.4 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001148.4

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Studies indicate that the covalent, flexible linker ensured that transcription factor TFIIB (TFIIB) was recruited stoichiometrically near its binding site on the RNA polymerase (Pol) II (Pol II) surface.
Title: Structure determination of transient transcription complexes.
TFIIB interaction with the CF1 complex and Pap1 is crucial for gene looping and transcriptional regulation.
Title: Evidence for a complex of transcription factor IIB with poly(A) polymerase and cleavage factor 1 subunits required for gene looping.
crystal structure of RNA polymerase II-TFIIB complex at 3.8 A resolution revealing carboxyl-terminal region of TFIIB, located above polymerase active center cleft but showing none of B finger; linker between amino- & carboxyl-terminal regions also seen
Title: Structure of an RNA polymerase II-TFIIB complex and the transcription initiation mechanism.
the crystal structure of the complete RNA polymerase II - transcription factor IIB complex at 4.3 A resolution
Title: RNA polymerase II-TFIIB structure and mechanism of transcription initiation.
TFIIB binds RNA Polymerase II at the terminator, which in turn associates with the promoter scaffold giving TFIIB a role in gene looping.
Title: A transcription-independent role for TFIIB in gene looping.
TBP1(E186D) temperature-sensitivity and slow-growth phenotypes might have been caused by insufficient amounts of Tbp1p for efficient transcription of the rRNA genes by RNA polymerase I
Title: TFIIB/SUA7(E202G) is an allele-specific suppressor of TBP1(E186D).
Results describe the architecture of transcription factor IIB and the the RNA polymerase II transcription preinitiation complex (PIC).
Title: Mapping the location of TFIIB within the RNA polymerase II transcription preinitiation complex: a model for the structure of the PIC.
TFIIB nuclear import is mediated by Kap114p
Title: Nuclear import of TFIIB is mediated by Kap114p, a karyopherin with multiple cargo-binding domains.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables RNA polymerase II complex binding	IDA Inferred from Direct Assay more info	PubMed
enables RNA polymerase II complex binding	IPI Inferred from Physical Interaction more info	PubMed
enables RNA polymerase II complex recruiting activity	IDA Inferred from Direct Assay more info	PubMed
enables RNA polymerase II complex recruiting activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables RNA polymerase II general transcription initiation factor activity	IBA Inferred from Biological aspect of Ancestor more info
enables TBP-class protein binding	IBA Inferred from Biological aspect of Ancestor more info
enables TBP-class protein binding	IDA Inferred from Direct Assay more info	PubMed
enables TBP-class protein binding	IEA Inferred from Electronic Annotation more info
enables TBP-class protein binding	IMP Inferred from Mutant Phenotype more info	PubMed
enables TBP-class protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables zinc ion binding	RCA inferred from Reviewed Computational Analysis more info	PubMed

Process	Evidence Code	Pubs
involved_in DNA-templated transcription initiation	IEA Inferred from Electronic Annotation more info
involved_in RNA polymerase II preinitiation complex assembly	IDA Inferred from Direct Assay more info	PubMed
involved_in RNA polymerase II preinitiation complex assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in gene expression	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of transcription regulatory region DNA binding	IDA Inferred from Direct Assay more info	PubMed
involved_in transcription initiation at RNA polymerase II promoter	IBA Inferred from Biological aspect of Ancestor more info
involved_in transcription open complex formation at RNA polymerase II promoter	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in transcription preinitiation complex assembly	IEA Inferred from Electronic Annotation more info
involved_in transcriptional start site selection at RNA polymerase II promoter	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info
part_of transcription preinitiation complex	IBA Inferred from Biological aspect of Ancestor more info
part_of transcription preinitiation complex	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: transcription factor TFIIB

NP_015411.1

Transcription factor TFIIB; a general transcription factor required for transcription initiation and start site selection by RNA polymerase II

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001148.4 Reference assembly

Range

710101..711138

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001184183.1 → NP_015411.1 TPA: transcription factor TFIIB [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_015411.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6W488, E9P939, P29055

UniProtKB/TrEMBL

A0A8H4BUZ0, B5VTN3, N1NXN0

Conserved Domains (1) summary

COG1405
Location:21 → 333

SUA7; Transcription initiation factor TFIIIB, Brf1 subunit/Transcription initiation factor TFIIB [Transcription]