ARO9 aromatic-amino-acid:2-oxoglutarate transaminase [Saccharomyces cerevisiae S288C] - Gene

Summary

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Gene symbol: ARO9
Gene description: aromatic-amino-acid:2-oxoglutarate transaminase
Primary source: SGD:S000001179
Locus tag: YHR137W
See related: AllianceGenome:SGD:S000001179
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables aromatic-amino-acid:2-oxoglutarate aminotransferase activity and kynurenine aminotransferase activity. Involved in L-phenylalanine biosynthetic process and tyrosine biosynthetic process. Located in cytoplasm and nucleus. Orthologous to human AADAT (aminoadipate aminotransferase). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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Location:: chromosome: VIII

Exon count:: 1

Sequence:: Chromosome: VIII; NC_001140.6 (375709..377250)

Chromosome VIII - NC_001140.6 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001140.6

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Study confirmed Aro8 and Aro9 as primary kynurenine aminotransferases (KATs) in S. cerevisiae and showed that the aro8Delta aro9Delta mutant cells lack kynurenic acid (KA) and showed that aro8Delta aro9Delta cells were sensitive to high concentrations of tryptophan (Trp). While expression of ARO9 rescued the sensitivity for Trp in the aro8Delta aro9Delta double mutant, addition of KA to the medium did not.
Title: Kynurenine aminotransferase activity of Aro8/Aro9 engage tryptophan degradation by producing kynurenic acid in Saccharomyces cerevisiae.
Known aromatic amino acid permeases play an insignificant role in the heat-induced expression of ARO9 and ARO10, suggesting that an increase in plasma membrane fluidity might be responsible for the influx of aromatic amino acids during heat shock stress.
Title: Activation of Aro80 transcription factor by heat-induced aromatic amino acid influx in Saccharomyces cerevisiae.
Crystals of ARO9 belong to the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 75.6 A, b = 117.5 A, c = 134.9 A.
Title: Crystallization and preliminary X-ray diffraction analysis of ARO9, an aromatic aminotransferase from Saccharomyces cerevisiae.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables 2-aminoadipate transaminase activity	IBA Inferred from Biological aspect of Ancestor more info
enables L-phenylalanine:2-oxoglutarate aminotransferase activity	IEA Inferred from Electronic Annotation more info
enables aromatic-amino-acid:2-oxoglutarate aminotransferase activity	IBA Inferred from Biological aspect of Ancestor more info
enables aromatic-amino-acid:2-oxoglutarate aminotransferase activity	IEA Inferred from Electronic Annotation more info
enables aromatic-amino-acid:2-oxoglutarate aminotransferase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables kynurenine aminotransferase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables kynurenine-oxoglutarate transaminase activity	IEA Inferred from Electronic Annotation more info
enables pyridoxal phosphate binding	IEA Inferred from Electronic Annotation more info
enables transaminase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in L-kynurenine catabolic process	IEA Inferred from Electronic Annotation more info
involved_in L-methionine salvage from methylthioadenosine	IEA Inferred from Electronic Annotation more info
involved_in L-phenylalanine biosynthetic process	IGI Inferred from Genetic Interaction more info	PubMed
involved_in aromatic amino acid family catabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in aromatic amino acid metabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in biosynthetic process	IEA Inferred from Electronic Annotation more info
involved_in lysine biosynthetic process via aminoadipic acid	IBA Inferred from Biological aspect of Ancestor more info
involved_in tyrosine biosynthetic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in tyrosine biosynthetic process	IGI Inferred from Genetic Interaction more info	PubMed

Component	Evidence Code	Pubs
located_in cytoplasm	HDA more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in nucleus	HDA more info	PubMed

General protein information

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Preferred Names: aromatic-amino-acid:2-oxoglutarate transaminase

NP_012005.1

EC 2.6.1.57

EC 2.6.1.7

Aromatic aminotransferase II; involved in tyrosine, phenylalanine, and methionine metabolism; involved with Aro8p in production of kynurenic acid to detoxify excess tryptophan

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001140.6 Reference assembly

Range

375709..377250

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001179267.1 → NP_012005.1 TPA: aromatic-amino-acid:2-oxoglutarate transaminase [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_012005.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D3DL86, P38840, P87291

UniProtKB/TrEMBL

A0A8H4BZU2, B3LSQ1, N1P167

Conserved Domains (1) summary

COG1167
Location:11 → 508

ARO8; DNA-binding transcriptional regulator, MocR family, contains an aminotransferase domain [Transcription, Amino acid transport and metabolism]