phoA alkaline phosphatase [Escherichia coli str. K-12 substr. MG1655] - Gene

Summary

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Gene symbol: phoA
Gene description: alkaline phosphatase
Primary source: ASAP:ABE-0001328
Locus tag: b0383
See related: ECOCYC:EG10727
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage: Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as: ECK0378; psiA
Summary: Arg22 is removed from the mature enzyme by Iap to form isozyme 3. [More information is available at EcoGene: EG10727]. Alkaline phosphatase is a dimer that occurs in three forms, depending upon the growth conditions of the cell. [More information is available at EcoCyc: EG10727].
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Genomic context

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Sequence:: NC_000913.3 (401747..403162)

NC_000913.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Cotranslational folding of alkaline phosphatase in the periplasm of Escherichia coli.
Title: Cotranslational folding of alkaline phosphatase in the periplasm of Escherichia coli.
Results support a model in which the Arg166 contributes to enzymatic phosphoryl transfer through binding interactions and through additional transition state stabilization.
Title: Arginine coordination in enzymatic phosphoryl transfer: evaluation of the effect of Arg166 mutations in Escherichia coli alkaline phosphatase.
RpoS contributes to the repression of alkaline phosphatase under excess of Phosphorus.
Title: Alkaline phosphatase as a reporter of sigma(S) levels and rpoS polymorphisms in different E. coli strains.
Data report the identification of a unique slipknot feature in the fold of a well-known thermostable protein, alkaline phosphatase (phoA).
Title: Identification of rare slipknots in proteins and their implications for stability and folding.
N-terminus verified by Edman degradation on mature peptide
Title: Amino acid sequence of Escherichia coli alkaline phosphatase.
the S102T mutation resulted in a shift in the rate-determining step in the mechanism allowing us to trap the covalent intermediate of the reaction in the crystal
Title: Trapping the tetrahedral intermediate in the alkaline phosphatase reaction by substitution of the active site serine with threonine.
differences in metal binding between bovine and Escherichia coli alkaline phosphatases
Title: Distinct structure and activity recoveries reveal differences in metal binding between mammalian and Escherichia coli alkaline phosphatases.
The structural and functional data together suggest that some of these distinct functional groups provide specific substrate binding interactions, whereas others tune active site itself to discriminate between phosphate diester and monoester substrates.
Title: Structural and functional comparisons of nucleotide pyrophosphatase/phosphodiesterase and alkaline phosphatase: implications for mechanism and evolution.
unfolded PhoA is targeted to the TatBC translocon of Escherichia coli
Title: Targeting of unfolded PhoA to the TAT translocon of Escherichia coli.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Gene Ontology Provided by EcoCyc

Function	Evidence Code	Pubs
enables alkaline phosphatase activity	IBA Inferred from Biological aspect of Ancestor more info
enables alkaline phosphatase activity	IDA Inferred from Direct Assay more info	PubMed
enables alkaline phosphatase activity	IEA Inferred from Electronic Annotation more info
enables hydrogenase (acceptor) activity	IDA Inferred from Direct Assay more info	PubMed
enables hydrolase activity	IEA Inferred from Electronic Annotation more info
enables magnesium ion binding	IDA Inferred from Direct Assay more info	PubMed
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables oxidoreductase activity, acting on phosphorus or arsenic in donors	IDA Inferred from Direct Assay more info	PubMed
enables phosphatase activity	IEA Inferred from Electronic Annotation more info
enables phosphoprotein phosphatase activity	IDA Inferred from Direct Assay more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables zinc ion binding	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in dephosphorylation	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within dephosphorylation	IDA Inferred from Direct Assay more info	PubMed
involved_in protein dephosphorylation	IDA Inferred from Direct Assay more info	PubMed

Component	Evidence Code	Pubs
located_in outer membrane-bounded periplasmic space	IDA Inferred from Direct Assay more info	PubMed
is_active_in periplasmic space	IBA Inferred from Biological aspect of Ancestor more info
located_in periplasmic space	IDA Inferred from Direct Assay more info	PubMed
located_in periplasmic space	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: alkaline phosphatase

NP_414917.2

EC 3.1.3.1

EC 3.1.3.102

EC 3.1.3.108

EC 3.1.3.11

EC 3.1.3.15

EC 3.1.3.18

EC 3.1.3.19

EC 3.1.3.20

EC 3.1.3.23

EC 3.1.3.3

EC 3.1.3.38

EC 3.1.3.41

EC 3.1.3.60

EC 3.1.3.68

EC 3.1.3.74

EC 3.1.3.75

EC 3.1.3.91

EC 3.1.3.99

EC 3.6.1.1

EC 3.6.1.25

EC 3.9.1.1

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_000913.3 Reference assembly

Range

401747..403162

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NP_414917.2 alkaline phosphatase [Escherichia coli str. K-12 substr. MG1655]

See identical proteins and their annotated locations for NP_414917.2

Status: PROVISIONAL

UniProtKB/TrEMBL

A0A775THJ3, A0A8I0CHG6

Conserved Domains (1) summary

PRK10518
Location:1 → 471

PRK10518; alkaline phosphatase; Provisional