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asnS asparagine--tRNA ligase [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 945555, updated on 2-May-2024

Summary

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Gene symbol
asnS
Gene description
asparagine--tRNA ligase
Primary source
ASAP:ABE-0003162
Locus tag
b0930
See related
ECOCYC:EG10094
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK0921; lcs; tss
Summary
Asparaginyl-tRNA synthetase (AsnRS) is a member of the family of aminoacyl-tRNA synthetases, which interpret the genetic code by covalently linking amino acids to their specific tRNA molecules. [More information is available at EcoCyc: EG10094].
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Genomic context

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Sequence:
NC_000913.3 (987585..988985, complement)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene aspartate aminotransferase Neighboring gene outer membrane porin F Neighboring gene nicotinate phosphoribosyltransferase Neighboring gene aminopeptidase N

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Asparaginyl-tRNA synthetase from Escherichia coli has significant sequence homologies with yeast aspartyl-tRNA synthetase. Anselme J, et al. Gene, 1989 Dec 14. PMID 2693216
  2. Regulation of gene expression at the translational level. The rescue factor reverses thermosensitive protein synthesis in N4316, a conditionally-lethal mutant of Escherichia coli defective in translation. Ganoza MC, et al. Eur J Biochem, 1989 Jan 2. PMID 2643515
  3. Tyr-426 of the Escherichia coli asparaginyl-tRNA synthetase, an amino acid in a C-terminal conserved motif, is involved in ATP binding. Anselme J, et al. FEBS Lett, 1991 Mar 11. PMID 2009959
  4. Asparaginyl-tRNA synthetase from the Escherichia coli temperature-sensitive strain HO202. A proline replacement in motif 2 is responsible for a large increase in Km for asparagine and ATP. Madern D, et al. FEBS Lett, 1992 Mar 24. PMID 1544480
  5. The asparaginyl-tRNA synthetase gene encodes one of the complementing factors for thermosensitive translation in the Escherichia coli mutant strain, N4316. Aoki H, et al. Eur J Biochem, 1992 Oct 15. PMID 1425658

See all (19) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. N-terminus verified by Edman degradation on mature peptide
    Title: Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12.
Submit: New GeneRIF Correction

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables ATP binding IEA
Inferred from Electronic Annotation
more info
  
enables ATP binding IMP
Inferred from Mutant Phenotype
more info
 PubMed 
enables aminoacyl-tRNA ligase activity IEA
Inferred from Electronic Annotation
more info
  
enables asparagine-tRNA ligase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables asparagine-tRNA ligase activity IEA
Inferred from Electronic Annotation
more info
  
enables asparagine-tRNA ligase activity IMP
Inferred from Mutant Phenotype
more info
 PubMed 
enables nucleic acid binding IEA
Inferred from Electronic Annotation
more info
  
enables protein homodimerization activity IDA
Inferred from Direct Assay
more info
 PubMed 
Process Evidence Code Pubs
involved_in asparaginyl-tRNA aminoacylation IBA
Inferred from Biological aspect of Ancestor
more info
  
acts_upstream_of_or_within asparaginyl-tRNA aminoacylation IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in asparaginyl-tRNA aminoacylation IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within asparaginyl-tRNA aminoacylation IMP
Inferred from Mutant Phenotype
more info
 PubMed 
acts_upstream_of_or_within tRNA aminoacylation for protein translation IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in translation IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
located_in cytoplasm IEA
Inferred from Electronic Annotation
more info
  

General protein information

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Preferred Names
asparagine--tRNA ligase
NP_415450.1

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    987585..988985 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_415450.1 asparagine--tRNA ligase [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_415450.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A6L6ZUR1, A0AA87JYJ6
    Conserved Domains (1) summary
    PRK03932
    Location:1 → 466
    asnC; asparaginyl-tRNA synthetase; Validated
Nucleotide Protein
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