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lsrK autoinducer-2 kinase [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 946069, updated on 2-May-2024

Summary

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Gene symbol
lsrK
Gene description
autoinducer-2 kinase
Primary source
ASAP:ABE-0005041
Locus tag
b1511
See related
ECOCYC:G6798
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK1504; ydeV
Summary
LsrK phosphorylates AI-2; phospo-AI-2 is retained in the cytoplasm. lsr operon induction by exogenous AI-2 requires LsrK, indicating phospho-AI-2, or a derivative, is the LsrR inducer. [More information is available at EcoGene: EG13804]. Based on its similarity to LsrK from Salmonella typhimurium , LsrK is predicted to phosphorylate the quorum-sensing autoinducer molecule AI-2 (shown for S. t. in ). [More information is available at EcoCyc: G6798].
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Genomic context

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Sequence:
NC_000913.3 (1598617..1600209, complement)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene serine/threonine-protein kinase toxin HipA Neighboring gene antitoxin/DNA-binding transcriptional repressor HipB Neighboring gene pseudo Neighboring gene DNA-binding transcriptional repressor LsrR Neighboring gene Autoinducer-2 ABC transporter ATP binding subunit Neighboring gene Autoinducer-2 ABC transporter membrane subunit LsrC

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Incorporating LsrK AI-2 quorum quenching capability in a functionalized biopolymer capsule. Rhoads MK, et al. Biotechnol Bioeng, 2018 Feb. PMID 28782813
  2. Increasing the soluble expression and crystallization of the Escherichia coli quorum-sensing protein LsrK. Ha JH, et al. Acta Crystallogr F Struct Biol Commun, 2017 May 1. PMID 28471356, Free PMC Article
  3. Involvement of quorum sensing and heat-stable enterotoxin a in cell damage caused by a porcine enterotoxigenic Escherichia coli strain. Zhu J, et al. Infect Immun, 2011 Apr. PMID 21300771, Free PMC Article
  4. Cross species quorum quenching using a native AI-2 processing enzyme. Roy V, et al. ACS Chem Biol, 2010 Feb 19. PMID 20025244
  5. Temporal regulation of enterohemorrhagic Escherichia coli virulence mediated by autoinducer-2. Bansal T, et al. Appl Microbiol Biotechnol, 2008 Apr. PMID 18256823

See all (22) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. LsrK was expressed and purified from E. coli with linker tags containing tyrosine or glutamine residues, attached to a chitosan film and a capsule, and used in quorum sensing studies.
    Title: Incorporating LsrK AI-2 quorum quenching capability in a functionalized biopolymer capsule.
  2. The soluble expression and crystallization of the Escherichia coli quorum-sensing protein LsrK has been described.
    Title: Increasing the soluble expression and crystallization of the Escherichia coli quorum-sensing protein LsrK.
  3. These results suggest the involvement of AI-2-mediated quorum sensing in K88(+) Enterotoxigenic Escherichia coli
    Title: Involvement of quorum sensing and heat-stable enterotoxin a in cell damage caused by a porcine enterotoxigenic Escherichia coli strain.
  4. Culture supernatants of E. coli and Staphylococcus aureus luxS mutants were found not to contain detectable levels of AI-2 activity.
    Title: LuxS-independent formation of AI-2 from ribulose-5-phosphate.
  5. AI-2-mediated regulation of enterohemorrhagic Escherichia coli chemotaxis and colonization, as well as temporal regulation of EHEC transcriptome by AI-2.
    Title: Temporal regulation of enterohemorrhagic Escherichia coli virulence mediated by autoinducer-2.
Submit: New GeneRIF Correction

Pathways from PubChem

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General gene information

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Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables autoinducer-2 kinase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables autoinducer-2 kinase activity IEA
Inferred from Electronic Annotation
more info
  
enables autoinducer-2 kinase activity ISS
Inferred from Sequence or Structural Similarity
more info
 PubMed 
enables kinase activity IEA
Inferred from Electronic Annotation
more info
  
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
Process Evidence Code Pubs
involved_in carbohydrate metabolic process IEA
Inferred from Electronic Annotation
more info
  
involved_in quorum sensing IEA
Inferred from Electronic Annotation
more info
  
involved_in quorum sensing IMP
Inferred from Mutant Phenotype
more info
 PubMed 
acts_upstream_of_or_within single-species biofilm formation IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
located_in cytoplasm IEA
Inferred from Electronic Annotation
more info
  

General protein information

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Preferred Names
autoinducer-2 kinase
NP_416028.3

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    1598617..1600209 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_416028.3 autoinducer-2 kinase [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_416028.3

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A6N8QFP7
    Conserved Domains (1) summary
    PRK10939
    Location:10 → 530
    PRK10939; autoinducer-2 (AI-2) kinase; Provisional
Nucleotide Protein
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