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gloA lactoylglutathione lyase [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 946161, updated on 4-Apr-2024

Summary

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Gene symbol
gloA
Gene description
lactoylglutathione lyase
Primary source
ASAP:ABE-0005520
Locus tag
b1651
See related
ECOCYC:G6891
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK1647
Summary
Glyoxalase I catalyzes the first of two sequential steps in the conversion of methylgloxal to D-lactate. [More information is available at EcoCyc: G6891].
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Genomic context

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Sequence:
NC_000913.3 (1727837..1728244)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene DNA-binding transcriptional repressor NemR Neighboring gene N-ethylmaleimide reductase Neighboring gene ribonuclease T Neighboring gene ATP-dependent helicase Lhr

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. 15N-1H HSQC NMR evidence for distinct specificity of two active sites in Escherichia coli glyoxalase I. Su Z, et al. Biochemistry, 2008 Dec 16. PMID 19053281
  2. Microbial glyoxalase enzymes: metalloenzymes controlling cellular levels of methylglyoxal. Sukdeo N, et al. Drug Metabol Drug Interact, 2008. PMID 18533363
  3. Glyoxalase I--structure, function and a critical role in the enzymatic defence against glycation. Thornalley PJ. Biochem Soc Trans, 2003 Dec. PMID 14641060
  4. Investigation of metal binding and activation of Escherichia coli glyoxalase I: kinetic, thermodynamic and mutagenesis studies. Clugston SL, et al. Biochem J, 2004 Jan 15. PMID 14556652, Free PMC Article
  5. An XAS investigation of product and inhibitor complexes of Ni-containing GlxI from Escherichia coli: mechanistic implications. Davidson G, et al. Biochemistry, 2001 Apr 17. PMID 11294624

See all (31) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Glyoxalase I alone among the core methylglyoxal protective systems is induced in response to methylglyoxal exposure.
    Title: Integrated stress response of Escherichia coli to methylglyoxal: transcriptional readthrough from the nemRA operon enhances protection through increased expression of glyoxalase I.
  2. We propose that cells have a cytosolic system consisting of the nemRA-gloA operon for the reduction of electrophiles, especially quinones and glyoxals, to maintain an appropriate intracellular redox balance.
    Title: Novel regulatory system nemRA-gloA for electrophile reduction in Escherichia coli K-12.
  3. N-terminus verified by Edman degradation on complete protein
    Title: Overproduction and characterization of a dimeric non-zinc glyoxalase I from Escherichia coli: evidence for optimal activation by nickel ions.
Submit: New GeneRIF Correction

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables lactoylglutathione lyase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables lactoylglutathione lyase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables lactoylglutathione lyase activity IEA
Inferred from Electronic Annotation
more info
  
enables lyase activity IEA
Inferred from Electronic Annotation
more info
  
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
enables nickel cation binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables protein homodimerization activity IDA
Inferred from Direct Assay
more info
 PubMed 
Process Evidence Code Pubs
involved_in methylglyoxal catabolic process to D-lactate via S-lactoyl-glutathione IBA
Inferred from Biological aspect of Ancestor
more info
  
acts_upstream_of_or_within methylglyoxal catabolic process to D-lactate via S-lactoyl-glutathione IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in response to toxic substance IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
is_active_in cytoplasm IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in cytosol IDA
Inferred from Direct Assay
more info
 PubMed 

General protein information

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Preferred Names
lactoylglutathione lyase
NP_416168.1

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    1727837..1728244
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_416168.1 lactoylglutathione lyase [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_416168.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A3L5H119, A0A3P5DQY7
    Conserved Domains (1) summary
    TIGR00068
    Location:2 → 135
    glyox_I; lactoylglutathione lyase
Nucleotide Protein
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