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sufS L-cysteine desulfurase [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 946185, updated on 6-Oct-2023

Summary

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Gene symbol
sufS
Gene description
L-cysteine desulfurase
Primary source
ASAP:ABE-0005610
Locus tag
b1680
See related
ECOCYC:G6906
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
csdB; ECK1676; ynhB
Summary
Fur regulon. [More information is available at EcoGene: EG13962]. SufS exhibits activity with respect to assembly of the ferredoxin iron-sulfur cluster in an in vitro assay . [More information is available at EcoCyc: G6906].
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Genomic context

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Sequence:
NC_000913.3 (1759303..1760523, complement)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene L,D-transpeptidase LdtE Neighboring gene sulfur carrier protein SufE Neighboring gene Fe-S cluster scaffold complex subunit SufD Neighboring gene Fe-S cluster scaffold complex subunit SufC Neighboring gene Fe-S cluster scaffold complex subunit SufB

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Structural evidence for a latch mechanism regulating access to the active site of SufS-family cysteine desulfurases. Dunkle JA, et al. Acta Crystallogr D Struct Biol, 2020 Mar 1. PMID 32133993, Free PMC Article
  2. Direct observation of intermediates in the SufS cysteine desulfurase reaction reveals functional roles of conserved active-site residues. Blahut M, et al. J Biol Chem, 2019 Aug 16. PMID 31248989, Free PMC Article
  3. Structural Evidence for Dimer-Interface-Driven Regulation of the Type II Cysteine Desulfurase, SufS. Dunkle JA, et al. Biochemistry, 2019 Feb 12. PMID 30571100, Free PMC Article
  4. The Role of SufS Is Restricted to Fe-S Cluster Biosynthesis in Escherichia coli. Bühning M, et al. Biochemistry, 2017 Apr 11. PMID 28323419
  5. The E. coli SufS-SufE sulfur transfer system is more resistant to oxidative stress than IscS-IscU. Dai Y, et al. FEBS Lett, 2012 Nov 16. PMID 23068614, Free PMC Article

See all (43) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. The beta-latch structural element of the SufS cysteine desulfurase mediates active site accessibility and SufE transpersulfurase positioning.
    Title: The β-latch structural element of the SufS cysteine desulfurase mediates active site accessibility and SufE transpersulfurase positioning.
  2. a detailed model of the SufS-catalyzed reaction from Cys binding to C-S bond cleavage and indicate that Arg-56, His-123, and Cys-364 are critical SufS residues in this C-S bond cleavage pathway.
    Title: Direct observation of intermediates in the SufS cysteine desulfurase reaction reveals functional roles of conserved active-site residues.
  3. SufS possesses a conserved beta-hairpin structure plays a role in mediating interactions with SufE protein. This influences communication between active sites of the SufS dimer coordinating the shift between desulfurase and transpersulfurase activities.
    Title: Structural Evidence for Dimer-Interface-Driven Regulation of the Type II Cysteine Desulfurase, SufS.
  4. The role of SufS is restricted to Fe-S cluster biosynthesis in Escherichia coli.
    Title: The Role of SufS Is Restricted to Fe-S Cluster Biosynthesis in Escherichia coli.
  5. These results show that dynamic changes may shift SufE to a sulfur-acceptor state that interacts more strongly with SufS.
    Title: SufE D74R Substitution Alters Active Site Loop Dynamics To Further Enhance SufE Interaction with the SufS Cysteine Desulfurase.
  6. Studies identified SufE-SufS and SufE-SufB interactions.
    Title: An in vivo method for characterization of protein interactions within sulfur trafficking systems of E. coli.
  7. N-terminus verified by Edman degradation on complete protein
    Title: A nifS-like gene, csdB, encodes an Escherichia coli counterpart of mammalian selenocysteine lyase. Gene cloning, purification, characterization and preliminary x-ray crystallographic studies.
Submit: New GeneRIF Correction

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables catalytic activity IEA
Inferred from Electronic Annotation
more info
  
enables cysteine desulfurase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables cysteine desulfurase activity IEA
Inferred from Electronic Annotation
more info
  
enables cysteine sulfinate desulfinase activity IEA
Inferred from Electronic Annotation
more info
  
enables hydrolase activity IEA
Inferred from Electronic Annotation
more info
  
enables lyase activity IEA
Inferred from Electronic Annotation
more info
  
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables protein homodimerization activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables pyridoxal phosphate binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables pyridoxal phosphate binding IEA
Inferred from Electronic Annotation
more info
  
enables selenocysteine lyase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables selenocysteine lyase activity IEA
Inferred from Electronic Annotation
more info
  
enables selenocysteine lyase activity NAS
Non-traceable Author Statement
more info
 PubMed 
enables transferase activity IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in cysteine metabolic process IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within iron-sulfur cluster assembly IDA
Inferred from Direct Assay
more info
 PubMed 
acts_upstream_of_or_within selenium compound metabolic process IGI
Inferred from Genetic Interaction
more info
 PubMed 
acts_upstream_of_or_within sulfur compound metabolic process IDA
Inferred from Direct Assay
more info
 PubMed 
Component Evidence Code Pubs
located_in cytoplasm IEA
Inferred from Electronic Annotation
more info
  

General protein information

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Preferred Names
L-cysteine desulfurase
NP_416195.1

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    1759303..1760523 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_416195.1 L-cysteine desulfurase [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_416195.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A8T6PK01
    Conserved Domains (1) summary
    PRK09295
    Location:1 → 406
    PRK09295; cysteine desulfurase SufS
Nucleotide Protein
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