U.S. flag

An official website of the United States government

Format

Send to:

Choose Destination

metG methionine--tRNA ligase [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 946643, updated on 2-May-2024

Summary

Go to the top of the page Help
Gene symbol
metG
Gene description
methionine--tRNA ligase
Primary source
ASAP:ABE-0006991
Locus tag
b2114
See related
ECOCYC:EG10586
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK2107
Summary
metG is an essential gene. [More information is available at EcoGene: EG10586]. MetG is a member of the family of aminoacyl tRNA synthetases, which interpret the genetic code by covalently linking amino acids to their specific tRNA molecules. [More information is available at EcoCyc: EG10586].
NEW
Try the new Gene table
Try the new Transcript table

Genomic context

Go to the top of the page Help
Sequence:
NC_000913.3 (2194300..2196333)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene DUF2574 domain-containing protein YehE Neighboring gene P-loop NTPase family protein Mrp Neighboring gene PF05406 family protein YehF Neighboring gene pseudo

Genomic regions, transcripts, and products

Go to the top of the page Help
Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

Go to the top of the pageHelp

Related articles in PubMed

  1. A single genomic copy of an engineered methionyl-tRNA synthetase enables robust incorporation of azidonorleucine into recombinant proteins in E. coli. Abdeljabbar DM, et al. J Am Chem Soc, 2009 Dec 2. PMID 19894713
  2. Switching from an induced-fit to a lock-and-key mechanism in an aminoacyl-tRNA synthetase with modified specificity. Schmitt E, et al. J Mol Biol, 2009 Dec 18. PMID 19837083, Free PMC Article
  3. Effect of a domain-spanning disulfide on aminoacyl-tRNA synthetase activity. Banerjee P, et al. Biochemistry, 2009 Oct 27. PMID 19772352
  4. Discovery of Escherichia coli methionyl-tRNA synthetase mutants for efficient labeling of proteins with azidonorleucine in vivo. Tanrikulu IC, et al. Proc Natl Acad Sci U S A, 2009 Sep 8. PMID 19706454, Free PMC Article
  5. Variations in clique and community patterns in protein structures during allosteric communication: investigation of dynamically equilibrated structures of methionyl tRNA synthetase complexes. Ghosh A, et al. Biochemistry, 2008 Nov 4. PMID 18842003

See all (50) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. N-terminus verified by Edman degradation on mature peptide
    Title: Methionyl-tRNA synthetase from Escherichia coli. Primary structure of the active crystallised tryptic fragment.
  2. Apo-MetRS structure shows a preference for binding methionine compared with the 19 other natural amino acids.
    Title: Selectivity and specificity of substrate binding in methionyl-tRNA synthetase.
Submit: New GeneRIF Correction

Interactions

Go to the top of the page Help
Products Interactant Other Gene Complex Source Pubs Description

General gene information

Go to the top of the page Help

Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables ATP binding IEA
Inferred from Electronic Annotation
more info
  
enables aminoacyl-tRNA ligase activity IEA
Inferred from Electronic Annotation
more info
  
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
enables methionine-tRNA ligase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables methionine-tRNA ligase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables methionine-tRNA ligase activity IEA
Inferred from Electronic Annotation
more info
  
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables protein homodimerization activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables tRNA binding IEA
Inferred from Electronic Annotation
more info
  
enables zinc ion binding IDA
Inferred from Direct Assay
more info
 PubMed 
Process Evidence Code Pubs
involved_in methionyl-tRNA aminoacylation IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in methionyl-tRNA aminoacylation IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within methionyl-tRNA aminoacylation IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in tRNA aminoacylation for protein translation IEA
Inferred from Electronic Annotation
more info
  
involved_in translation IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
located_in cytoplasm IEA
Inferred from Electronic Annotation
more info
  
located_in cytosol HDA PubMed 
is_active_in cytosol IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in membrane HDA PubMed 

General protein information

Go to the top of the page Help
Preferred Names
methionine--tRNA ligase
NP_416617.1

NCBI Reference Sequences (RefSeq)

Go to the top of the page Help

NEW Try the new Transcript table

Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    2194300..2196333
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_416617.1 methionine--tRNA ligase [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_416617.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A1X3JDZ1, A0A3W4K111, F4TI82
    Conserved Domains (1) summary
    PRK00133
    Location:6 → 677
    metG; methionyl-tRNA synthetase; Reviewed
Nucleotide Protein
Heading Accession and Version

Gene LinkOut

The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

Other Literature Sources
Molecular Biology Databases
Research Materials