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ptsI PTS enzyme I [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 946879, updated on 11-Apr-2024

Summary

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Gene symbol
ptsI
Gene description
PTS enzyme I
Primary source
ASAP:ABE-0007967
Locus tag
b2416
See related
ECOCYC:EG10789
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ctr; ECK2411
Summary
Enzyme I and HPr are the two sugar-non-specific protein constituents of the PTS. [More information is available at EcoCyc: EG10789].
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Genomic context

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Sequence:
NC_000913.3 (2534066..2535793)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene cysteine synthase A Neighboring gene phosphocarrier protein HPr Neighboring gene Enzyme IIA(Glc) Neighboring gene pyridoxine/pyridoxal/pyridoxamine kinase

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. The oligomerization state of bacterial enzyme I (EI) determines EI's allosteric stimulation or competitive inhibition by α-ketoglutarate. Nguyen TT, et al. J Biol Chem, 2018 Feb 16. PMID 29317499, Free PMC Article
  2. Calorimetric and spectroscopic investigation of the interaction between the C-terminal domain of Enzyme I and its ligands. Yun YJ, et al. Protein Sci, 2012 Nov. PMID 22936614, Free PMC Article
  3. The monomer/dimer transition of enzyme I of the Escherichia coli phosphotransferase system. Patel HV, et al. J Biol Chem, 2006 Jun 30. PMID 16547355
  4. Properties of the C-terminal domain of enzyme I of the Escherichia coli phosphotransferase system. Patel HV, et al. J Biol Chem, 2006 Jun 30. PMID 16547354
  5. Subcellular distribution of enzyme I of the Escherichia coli phosphoenolpyruvate:glycose phosphotransferase system depends on growth conditions. Patel HV, et al. Proc Natl Acad Sci U S A, 2004 Dec 14. PMID 15557553, Free PMC Article

See all (221) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. When the oligomerization state of EI is shifted toward the dimeric species, alpha-ketoglutarate functions as a competitive inhibitor of EI.
    Title: The oligomerization state of bacterial enzyme I (EI) determines EI's allosteric stimulation or competitive inhibition by α-ketoglutarate.
  2. Study elucidated the structure of the NPr:EIN Ntr complex using a hybrid approach. The structure has a similar fold to the homologous HPr:EIN sugar complex, but differs in surface potential, which explains the difference in specificity between the two evolutionarily related enzyme pairs.
    Title: Structure of the NPr:EIN(Ntr) Complex: Mechanism for Specificity in Paralogous Phosphotransferase Systems.
  3. Isolated a stable recombinant C-terminal domain of Enzyme I (EIC) of Escherichia coli and characterized its interaction with the N-terminal domain of Enzyme I (EIN) and also with various ligands.
    Title: Calorimetric and spectroscopic investigation of the interaction between the C-terminal domain of Enzyme I and its ligands.
  4. N-terminus verified by Edman degradation on complete protein
    Title: Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12.
  5. crystal structure reveals a dimeric protein where each subunit comprises three domains
    Title: Structure of phosphorylated enzyme I, the phosphoenolpyruvate:sugar phosphotransferase system sugar translocation signal protein.
  6. EI and EI-H189Q each comprise a multiplicity of conformers and progressively fewer conformers as they dimerize and bind Mg(2+) and finally PEP
    Title: The monomer/dimer transition of enzyme I of the Escherichia coli phosphotransferase system.
  7. the phosphotransfer rate increases approximately 25-fold when HPr is added to a mixture of EI-N, EI-C, and PEP(Mg(2+))
    Title: Properties of the C-terminal domain of enzyme I of the Escherichia coli phosphotransferase system.
  8. Subcellular distribution depends on growth conditions
    Title: Subcellular distribution of enzyme I of the Escherichia coli phosphoenolpyruvate:glycose phosphotransferase system depends on growth conditions.
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Pathways from PubChem

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Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables catalytic activity IEA
Inferred from Electronic Annotation
more info
  
enables identical protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables kinase activity IEA
Inferred from Electronic Annotation
more info
  
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
enables phosphoenolpyruvate-protein phosphotransferase activity IEA
Inferred from Electronic Annotation
more info
  
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables transferase activity IEA
Inferred from Electronic Annotation
more info
  
enables transferase activity, transferring phosphorus-containing groups IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in organic substance transport IEA
Inferred from Electronic Annotation
more info
  
involved_in phosphoenolpyruvate-dependent sugar phosphotransferase system IEA
Inferred from Electronic Annotation
more info
  
involved_in phosphorylation IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
located_in cytoplasm IEA
Inferred from Electronic Annotation
more info
  
located_in cytosol HDA PubMed 

General protein information

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Preferred Names
PTS enzyme I

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    2534066..2535793
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_416911.1 PTS enzyme I [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_416911.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A376J2V9, A0A8S7J1Q8, A0A8S7L030, A0AA87KBA3
    Conserved Domains (1) summary
    PRK11177
    Location:1 → 575
    PRK11177; phosphoenolpyruvate-protein phosphotransferase PtsI
Nucleotide Protein
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