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arnT lipid IVA 4-amino-4-deoxy-L-arabinosyltransferase [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 947297, updated on 23-Nov-2023

Summary

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Gene symbol
arnT
Gene description
lipid IVA 4-amino-4-deoxy-L-arabinosyltransferase
Primary source
ASAP:ABE-0007464
Locus tag
b2257
See related
ECOCYC:G7170
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK2250; pmrK; yfbI
Summary
Inner membane protein. basRS regulon. [More information is available at EcoGene: EG14093]. ArnT is the 4-amino-4-deoxy-L-arabinose (L-Ara4N) transferase that catalyzes addition of L-Ara4N to lipid A under some conditions (polymyxin resistant mutants) . [More information is available at EcoCyc: G7170].
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Genomic context

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Sequence:
NC_000913.3 (2370908..2372560)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene undecaprenyl-phosphate 4-deoxy-4-formamido-L-arabinose transferase Neighboring gene fused UDP-4-amino-4-deoxy-L-arabinose formyltransferase/UDP-glucuronate dehydrogenase Neighboring gene putative 4-deoxy-4-formamido-L-arabinose-phosphoundecaprenol deformylase ArnD Neighboring gene undecaprenyl-phosphate-alpha-L-Ara4N flippase - ArnE subunit Neighboring gene undecaprenyl-phosphate-alpha-L-Ara4N flippase - ArnF subunit Neighboring gene signal transduction protein PmrD Neighboring gene o-succinylbenzoate--CoA ligase Neighboring gene o-succinylbenzoate synthase

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Purification and characterization of the L-Ara4N transferase protein ArnT from Salmonella typhimurium. Bretscher LE, et al. Protein Expr Purif, 2006 Mar. PMID 16226890
  2. An inner membrane enzyme in Salmonella and Escherichia coli that transfers 4-amino-4-deoxy-L-arabinose to lipid A: induction on polymyxin-resistant mutants and role of a novel lipid-linked donor. Trent MS, et al. J Biol Chem, 2001 Nov 16. PMID 11535604
  3. Resistance to the antimicrobial peptide polymyxin requires myristoylation of Escherichia coli and Salmonella typhimurium lipid A. Tran AX, et al. J Biol Chem, 2005 Aug 5. PMID 15951433
  4. An undecaprenyl phosphate-aminoarabinose flippase required for polymyxin resistance in Escherichia coli. Yan A, et al. J Biol Chem, 2007 Dec 7. PMID 17928292, Free PMC Article
  5. A formyltransferase required for polymyxin resistance in Escherichia coli and the modification of lipid A with 4-Amino-4-deoxy-L-arabinose. Identification and function oF UDP-4-deoxy-4-formamido-L-arabinose. Breazeale SD, et al. J Biol Chem, 2005 Apr 8. PMID 15695810

See all (27) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Purified recombinant ArnT protein was shown to be highly alpha-helical as determined by circular dichroism analysis.
    Title: Purification and characterization of the L-Ara4N transferase protein ArnT from Salmonella typhimurium.
Submit: New GeneRIF Correction

Pathways from PubChem

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General gene information

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Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables 4-amino-4-deoxy-L-arabinose transferase activity IEA
Inferred from Electronic Annotation
more info
  
enables glycosyltransferase activity IEA
Inferred from Electronic Annotation
more info
  
enables mannosyltransferase activity IEA
Inferred from Electronic Annotation
more info
  
enables pentosyltransferase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables pentosyltransferase activity IEA
Inferred from Electronic Annotation
more info
  
enables pentosyltransferase activity IMP
Inferred from Mutant Phenotype
more info
 PubMed 
enables transferase activity IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in lipid A biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
involved_in lipid metabolic process IEA
Inferred from Electronic Annotation
more info
  
involved_in lipopolysaccharide biosynthetic process IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in lipopolysaccharide biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within lipopolysaccharide biosynthetic process IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in protein O-linked glycosylation IEA
Inferred from Electronic Annotation
more info
  
involved_in response to iron(III) ion IBA
Inferred from Biological aspect of Ancestor
more info
  
acts_upstream_of_or_within response to iron(III) ion IEP
Inferred from Expression Pattern
more info
 PubMed 
acts_upstream_of_or_within response to iron(III) ion IGI
Inferred from Genetic Interaction
more info
 PubMed 
Component Evidence Code Pubs
located_in membrane IEA
Inferred from Electronic Annotation
more info
  
is_active_in plasma membrane IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in plasma membrane IDA
Inferred from Direct Assay
more info
 PubMed 
located_in plasma membrane IEA
Inferred from Electronic Annotation
more info
  

General protein information

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Preferred Names
lipid IVA 4-amino-4-deoxy-L-arabinosyltransferase
NP_416760.1

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    2370908..2372560
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_416760.1 lipid IVA 4-amino-4-deoxy-L-arabinosyltransferase [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_416760.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A8S7R9Z6
    Conserved Domains (1) summary
    PRK13279
    Location:1 → 549
    arnT; lipid IV(A) 4-amino-4-deoxy-L-arabinosyltransferase
Nucleotide Protein
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