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hycE formate hydrogenlyase subunit HycE [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 947396, updated on 11-Apr-2024

Summary

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Gene symbol
hycE
Gene description
formate hydrogenlyase subunit HycE
Primary source
ASAP:ABE-0008944
Locus tag
b2721
See related
ECOCYC:EG10478
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK2716; hevE
Summary
FhlA regulon. [More information is available at EcoGene: EG10478]. HycE is the large subunit of hydrogenase 3 . [More information is available at EcoCyc: EG10478].
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Genomic context

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Sequence:
NC_000913.3 (2844762..2846471, complement)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene hydrogenase 3 maturation protease Neighboring gene formate hydrogenlyase assembly protein Neighboring gene formate hydrogenlyase subunit HycG Neighboring gene formate hydrogenlyase subunit HycF Neighboring gene formate hydrogenlyase subunit HycD Neighboring gene formate hydrogenlyase subunit HycC Neighboring gene formate hydrogenlyase subunit HycB

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Protein engineering of hydrogenase 3 to enhance hydrogen production. Maeda T, et al. Appl Microbiol Biotechnol, 2008 May. PMID 18335216
  2. Redox sensing by Escherichia coli: effects of copper ions as oxidizers on proton-coupled membrane transport. Kirakosyan G, et al. Bioelectrochemistry, 2007 Jan. PMID 16713752
  3. Nickel incorporation into hydrogenase 3 from Escherichia coli requires the precursor form of the large subunit. Binder U, et al. Arch Microbiol, 1996 Jan. PMID 8639025
  4. Copper (II) ions affect Escherichia coli membrane vesicles' SH-groups and a disulfide-dithiol interchange between membrane proteins. Kirakosyan G, et al. Cell Biochem Biophys, 2008. PMID 18458828
  5. Analysis of the cleavage site specificity of the endopeptidase involved in the maturation of the large subunit of hydrogenase 3 from Escherichia coli. Theodoratou E, et al. Arch Microbiol, 2000 Feb. PMID 10795682

See all (37) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Hydrogen-dependent reduction of benzyl viologen in hydrogenases HyaB, HybC and HybE is dependent on the [Fe-S]-cluster-containing small subunit.
    Title: Efficient electron transfer from hydrogen to benzyl viologen by the [NiFe]-hydrogenases of Escherichia coli is dependent on the coexpression of the iron-sulfur cluster-containing small subunit.
  2. N-terminus verified by Edman degradation on complete protein
    Title: Maturation of the large subunit (HYCE) of Escherichia coli hydrogenase 3 requires nickel incorporation followed by C-terminal processing at Arg537.
Submit: New GeneRIF Correction

Pathways from PubChem

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Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables 4 iron, 4 sulfur cluster binding IEA
Inferred from Electronic Annotation
more info
  
enables NAD binding IEA
Inferred from Electronic Annotation
more info
  
enables NADH dehydrogenase (ubiquinone) activity IEA
Inferred from Electronic Annotation
more info
  
enables iron-sulfur cluster binding IEA
Inferred from Electronic Annotation
more info
  
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
enables nickel cation binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables nickel cation binding IEA
Inferred from Electronic Annotation
more info
  
enables nickel cation binding RCA
inferred from Reviewed Computational Analysis
more info
 PubMed 
enables oxidoreductase activity IEA
Inferred from Electronic Annotation
more info
  
enables oxidoreductase activity, acting on NAD(P)H IEA
Inferred from Electronic Annotation
more info
  
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables quinone binding IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in anaerobic electron transport chain IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in anaerobic respiration IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in formate oxidation IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in glucose catabolic process IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in transmembrane transport IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
part_of formate dehydrogenase complex IPI
Inferred from Physical Interaction
more info
 PubMed 

General protein information

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Preferred Names
formate hydrogenlyase subunit HycE
NP_417201.1

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    2844762..2846471 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_417201.1 formate hydrogenlyase subunit HycE [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_417201.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A6D0IAP2
    Conserved Domains (2) summary
    COG3261
    Location:172 → 552
    HycE2; Ni,Fe-hydrogenase III large subunit [Energy production and conversion]
    COG3262
    Location:2 → 166
    HycE1; Ni,Fe-hydrogenase III component G [Energy production and conversion]
Nucleotide Protein
Heading Accession and Version

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