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ansB L-asparaginase 2 [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 947454, updated on 4-Feb-2024

Summary

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Gene symbol
ansB
Gene description
L-asparaginase 2
Primary source
ASAP:ABE-0009700
Locus tag
b2957
See related
ECOCYC:EG10046
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK2952
Summary
There are two asparaginase enzymes in Escherichia coli, I and II. [More information is available at EcoCyc: EG10046].
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Genomic context

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Sequence:
NC_000913.3 (3099682..3100728, complement)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene dITP/XTP pyrophosphatase Neighboring gene heme chaperone HemW Neighboring gene DUF1202 domain-containing protein YggM Neighboring gene DUF2884 domain-containing protein YggN Neighboring gene putative ribosome assembly factor YggL Neighboring gene tRNA m(7)G46 methyltransferase

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Revealing Escherichia coli type II L-asparaginase active site flexible loop in its open, ligand-free conformation. Maggi M, et al. Sci Rep, 2021 Sep 23. PMID 34556749, Free PMC Article
  2. Biophysical characterization of two commercially available preparations of the drug containing Escherichia coli L-Asparaginase 2. de Araújo TS, et al. Biophys Chem, 2021 Apr. PMID 33607531
  3. Application of repeated aspartate tags to improving extracellular production of Escherichia coli L-asparaginase isozyme II. Kim SK, et al. Enzyme Microb Technol, 2015 Nov. PMID 26320714
  4. Refolding and purification of recombinant L-asparaginase from inclusion bodies of E. coli into active tetrameric protein. Upadhyay AK, et al. Front Microbiol, 2014. PMID 25309524, Free PMC Article
  5. Crystal structure of active site mutant of antileukemic L-asparaginase reveals conserved zinc-binding site. Borek D, et al. FEBS J, 2014 Sep. PMID 25040257

See all (40) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Revealing Escherichia coli type II L-asparaginase active site flexible loop in its open, ligand-free conformation.
    Title: Revealing Escherichia coli type II L-asparaginase active site flexible loop in its open, ligand-free conformation.
  2. Biophysical characterization of two commercially available preparations of the drug containing Escherichia coli L-Asparaginase 2.
    Title: Biophysical characterization of two commercially available preparations of the drug containing Escherichia coli L-Asparaginase 2.
  3. deletion of the gspDE gene reduced extracellular production of asparaginase isozyme II, indicating that secretion of recombinant asparaginase isozyme II was partially ascribed to the recognition by the general secretion machinery
    Title: Application of repeated aspartate tags to improving extracellular production of Escherichia coli L-asparaginase isozyme II.
  4. The crystal structure of active site variants of E coli antileukemic L-asparaginase reveals a conserved zinc-binding site.
    Title: Crystal structure of active site mutant of antileukemic L-asparaginase reveals conserved zinc-binding site.
  5. N-terminus verified by Edman degradation on mature peptide
    Title: The primary structure of L-asparaginase from Escherichia coli.
Submit: New GeneRIF Correction

Pathways from PubChem

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Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables asparaginase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables asparaginase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables asparaginase activity IEA
Inferred from Electronic Annotation
more info
  
enables hydrolase activity IEA
Inferred from Electronic Annotation
more info
  
enables identical protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
Process Evidence Code Pubs
involved_in amino acid metabolic process IEA
Inferred from Electronic Annotation
more info
  
involved_in asparagine catabolic process IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in asparagine metabolic process IEA
Inferred from Electronic Annotation
more info
  
involved_in protein homotetramerization IDA
Inferred from Direct Assay
more info
 PubMed 
Component Evidence Code Pubs
located_in outer membrane-bounded periplasmic space IDA
Inferred from Direct Assay
more info
 PubMed 
is_active_in periplasmic space IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in periplasmic space IEA
Inferred from Electronic Annotation
more info
  
part_of protein-containing complex IDA
Inferred from Direct Assay
more info
 PubMed 

General protein information

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Preferred Names
L-asparaginase 2
NP_417432.1

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    3099682..3100728 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_417432.1 L-asparaginase 2 [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_417432.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A5C0PZP7
    Conserved Domains (1) summary
    PRK11096
    Location:2 → 348
    ansB; L-asparaginase II; Provisional
Nucleotide Protein
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