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speB agmatinase [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 947715, updated on 2-May-2024

Summary

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Gene symbol
speB
Gene description
agmatinase
Primary source
ASAP:ABE-0009632
Locus tag
b2937
See related
ECOCYC:EG10960
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK2932
Summary
HT_Cmplx35_Cyt: [More information is available at EcoGene: EG10960]. Agmatinase (SpeB) carries out the second step in putrescine biosynthesis, generating the pathway's putrescine product. [More information is available at EcoCyc: EG10960].
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Genomic context

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Sequence:
NC_000913.3 (3082877..3083797, complement)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene transketolase 1 Neighboring gene metalloprotease LoiP Neighboring gene biosynthetic arginine decarboxylase Neighboring gene acid stress response protein YqgB

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Structure of the E. coli agmatinase, SPEB. Chitrakar I, et al. PLoS One, 2021. PMID 33857156, Free PMC Article
  2. Kinetic studies and site-directed mutagenesis of Escherichia coli agmatinase. A role for Glu274 in binding and correct positioning of the substrate guanidinium group. Carvajal N, et al. Arch Biochem Biophys, 2004 Oct 15. PMID 15369817
  3. Studies on the interaction of Escherichia coli agmatinase with manganese ions: structural and kinetic studies of the H126N and H151N variants. Salas M, et al. J Inorg Biochem, 2004 Jun. PMID 15149812
  4. Insights into the reaction mechanism of Escherichia coli agmatinase by site-directed mutagenesis and molecular modelling. Salas M, et al. Eur J Biochem, 2002 Nov. PMID 12423350
  5. Evidence that histidine-163 is critical for catalytic activity, but not for substrate binding to Escherichia coli agmatinase. Carvajal N, et al. Biochem Biophys Res Commun, 1999 Oct 14. PMID 10527864

See all (32) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Structure of the E. coli agmatinase, SPEB.
    Title: Structure of the E. coli agmatinase, SPEB.
Submit: New GeneRIF Correction

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables agmatinase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables agmatinase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables agmatinase activity IEA
Inferred from Electronic Annotation
more info
  
enables hydrolase activity IEA
Inferred from Electronic Annotation
more info
  
enables hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amidines IEA
Inferred from Electronic Annotation
more info
  
enables identical protein binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables manganese ion binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables manganese ion binding IEA
Inferred from Electronic Annotation
more info
  
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in putrescine biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
involved_in putrescine biosynthetic process from arginine IEA
Inferred from Electronic Annotation
more info
  
involved_in putrescine biosynthetic process from arginine, using agmatinase IBA
Inferred from Biological aspect of Ancestor
more info
  
acts_upstream_of_or_within putrescine biosynthetic process from arginine, using agmatinase IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in spermidine biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
located_in cytosol IDA
Inferred from Direct Assay
more info
 PubMed 

General protein information

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Preferred Names
agmatinase
NP_417412.1

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    3082877..3083797 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_417412.1 agmatinase [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_417412.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A376UHZ9
    Conserved Domains (1) summary
    PRK02190
    Location:7 → 306
    PRK02190; agmatinase; Provisional
Nucleotide Protein
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